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Embryo-callus regenerated hybrids and amphidiploids of barley cultivars x rye inbred lines

100%

Wojciechowska B., Pudelska H.

Journal of Applied Genetics

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1995

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tom 36

|

nr 4

In the presented hybridization programme of barley cultivars and rye inbred lines including 48 cross combinations the seed set ranged from 3.13 to 92.98%, while embryos were formed in 0.74 to 36.36% in successful pollinations. Sixty five plants were generated by embryo callus culture and one - by embryo culture without callus formation. The hybrids had somatic chromosome numbers 2n=14 (60 plants) and 2n=28 (6 plants). Plants obtained via embryo callus culture showed good vegetative vigour and well-developed root system. Spike morphology of all plants resembled that of rye. Meiosis in 17 diploids showed 0.13-0.63 barley-barley and rye-rye bivalents with a chiasma frequency of 0.14-0.69 per cell. The heteromorphic bivalent-like configurations occurred in five plants in 0.01-0.02 per cell. The amphidiploids had 7.79-10.71 barley-barley and rye-rye bivalents with a chiasma frequency of 9.36-17.75 per cell. All plants, with 14 and 28 chromosomes, were completely sterile both in backcrosses and when selfed.

2

Interspecific and intergeneric hybrids of the Lolium - Festuca complex obtained in Poland in the years 1964-1994 and maintained in the collection at the Institute of Plant Genetics in Poznan

86%

Zwierzykowski Z.

Journal of Applied Genetics

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1996

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tom 37

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nr 1

In the years 1964-1994 an extensive programme of wide hybridization within Ihe. Lolium-Festuca complex was carried out in Poland. Six Lolium (ryegrass) and five Festuca (fescue) species at different ploidy level were used for crosses. Hybrids were obtained from 72 cross combinations: 19 interspecific (five Lolium × Lolium and 14 Festuca × Festuca), 51 intergeneric (39 Lolium × Festuca and 12 Festuca × Lolium) and two trispecific (Lolium × Festuca × Festuca), most of them being derived from crosses of four important forage grass species: Lolium multiflorum, L. perenne, Festuca pratensis and F. arundinacea. Interspecific and intergeneric Lolium-F estuca hybrids from 50 cross combinations are maintained at present in the collection of the Institute of Plant Genetics in Poznań. This article presents a complete list of Lolium-Festuca hybrids obtained in Poland in the years 1964-1994 and maintained in the collection. The available literature concerning these hybrids is cited.

3

Isozymes in Aegilops kotschyi and Ae.biuncialis x Secale cereale hybrids and Ae.kostchyi x S.cereale amphiploids in relation to their parents

72%

Kalinowski A., Wojciechowska B., Borzyszkowska E.

Journal of Applied Genetics

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2003

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tom 44

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nr 1

4

Performance of interspecific and intergeneric hybrids of Triticum aestivum L. for wheat improvement. Part I. Performance of winter generations F3-F5 of T.aestivum L. with Triticum [2x,4x], Aegilops [2x] and Elymus [4x] species in respect of some characters of spike

72%

Pilch J.

Plant Breeding and Seed Science

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1996

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tom 40

|

nr 3-4

5

Pollen morphology and two-dimensional patterns of pollen coat and protoplast proteins in Aegilops kotschyi x Secale cereale amphiploids

72%

Kalinowski A., Klimko M., Wojciechowska B.

Acta Biologica Cracoviensia. Series Botanica

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2005

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tom 47

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nr 2

Amphiploid pollen of Aegilops kotschyi x Secale cereale was compared to the parental form by SEM, and comparatively measured using light microscopy. Pollen grains of amphiploids were larger and more variable in total and pore diameter than the parents. Amphiploid pollen was prolate, subprolate and prolate-spheroidal in shape. The exine of Ae. kotschyi AK-2 and AK-3 had a delicate verrucate surface, whereas Secale cereale S14 exine had a verrucate surface. Amphiploid pollen grain surfaces were more or less similar to those of the parents: delicate verrucate, verrucate and well verrucate. The sculpture of parental and amphiploid pollen grains showed conspicuous granulation. All amphiploids produced pollen with one pore with an operculum, surrounded by a well-defined annulus. Sporophytically produced peptides from the pollen coat and gametophytically produced peptides from the protoplast were analyzed separately by two-dimensional gel electrophoresis. Two accessions ofAe. kotschyi (AK-2, AK-3) showed differences in the 2-D patterns of peptides from both the pollen coat and the protoplast. The majority of pollen coat and protoplast peptides of the parents were detected in the amphiploids, but a number of parental peptides were absent. All the amphiploids possessed peptides in their pollen coat and protoplast not detected in the corresponding pollen fractions of the parents. No relation between colchicine and in vitro amphiploid production and its 2-D patterns was observed. The results of pollen morphology and pollen protein analysis are convergent.

6

Chromosome pairing in triploid intergeneric hybrids of Festuca pratensis with Lolium multiflorum, revealed by GISH

72%

Kosmala A., Zwierzykowska E., Zwierzykowski Z.

Journal of Applied Genetics

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2006

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tom 47

|

nr 3

Genomic in situ hybridisation (GISH) was used to reveal chromosome pairing in two partly fertile, triploid (2n = 3x = 21) hybrids obtained by crossing the diploid (2n = 2x = 14) Festuca pratensis Huds. (designated FpFp), used as a female parent, with the autotetraploid (2n = 4x = 28) Lolium multiflorum Lam. (designated LmLmLmLm), used as a male parent. The pattern of chromosome pairing calculated on the basis of the mean values of chromosome configurations identified in all 100 PMCs analysed, was: 0.71I Lm + 2.24I Fp + 2.18II Lm/Lm + 0.54II Lm/Fp + 4.18III Lm/Lm/Fp. A relatively high number of Lm/Lm bivalents and Fp univalents, and a low number of Lm/Fp bivalents and Lm univalents indicated that the pairing was preferential between L. multiflorum chromosomes. Other observations regarding chromosome pairing within the Lm/Lm/Fp trivalents also confirmed this preferential pairing in the analysed triploids, as the Fp chromosome was not randomly located in the chain- and frying-pan-shaped trivalents. The similarities and differences in chromosome pairing at metaphase I and the level of preferential pairing between Lolium chromosomes in the different triploid Lolium-Festuca hybrids are discussed.

7

Lista mieszańców storczykowatych (Orchidaceae) we florze Polski

58%

Bernacki L.

Acta Biologica Silesiana

|

1989

|

tom 11

8

Mozliwosci wykorzystania mieszancow miedzygatunkowych i miedzyrodzajowych w hodowli pszenicy jarej

58%

Pilch J., Walag H., Karkoszka U.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

|

1992

|

nr 181-182

47-51

9

Zarodnikowanie szczepow z rodzaju Saccharomyces i Schwanniomyces oraz produktow ich fuzji

58%

Kawa-Rygielska J., Dziuba E.

Acta Scientiarum Polonorum. Biotechnologia

|

2003

|

tom 02

|

nr 1-2

59-65

10

Charakterystyka zbiorów kolekcyjnych pszenżyta jako mieszańca międzyrodzajowego

58%

Kociuba W.

Zeszyty Problemowe Postępów Nauk Rolniczych

|

2010

|

tom 555

Systematyczne gromadzenie materiałów kolekcyjnych pszenżyta rozpoczęto w 1982 roku. Początkowo gromadzono głównie rody hodowlane otrzymane w krajowych ośrodkach hodowli pszenżyta. Następnie sprowadzono materiały ze światowych banków genów: Beltsville, Gatersleben, VIR. Do kolekcji włączono także interesujące materiały mieszańcowe otrzymywane w placówkach badawczych. Obecnie kolekcja liczy 2333 obiektów, w tym 1339 pszenżyta ozimego i 994 pszenżyta jarego. Waloryzacja prowadzona jest w 4-letnim cyklu doświadczeń potowych według jednolitej metodyki. Zgromadzone materiały kolekcyjne reprezentują duże spektrum zmienności zarówno cech morfologicznych, jak i użytkowych. Duże zróżnicowanie badanych obiektów kolekcyjnych dotyczy zwłaszcza takich cech, jak: wysokość roślin, liczba i masa ziaren z kłosa, zawartość białka w ziarnie oraz potowej odporności na mączniaka właściwego i rdzę brunatną.

11

Variability of ribosomal DNA sites in Festuca pratensis, Lolium perenne, and their intergeneric hybrids, revealed by FISH and GISH

58%

Ksiazczyk T., Taciak M., Zwierzykowski Z.

Journal of Applied Genetics

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2010

|

tom 51

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nr 4

This study focuses on the variability of chromosomal location and number of ribosomal DNA (rDNA) sites in some diploid and autotetraploid Festuca pratensis and Lolium perenne cultivars, as well as on identification of rDNA-bearing chromosomes in their triploid and tetraploid F. pratensis × L. perenne hybrids. The rDNA loci were mapped using fluorescence in situ hybridization (FISH) with 5S and 25S rDNA probes, and the origin of parental genomes was verified by genomic in situ hybridization (GISH) with L. perenne genomic DNA as a probe, and F. pratensis genomic DNA as a block. FISH detected variation in the number and chromosomal location of both 5S and 45S rDNA sites. In F. pratensis mostly additional signals of 5S rDNA loci occurred, as compared with standard F. pratensis karyotypes. Losses of 45S rDNA loci were more frequent in L. perenne cultivars and intergeneric hybrids. Comparison of the F. pratensis and L. perenne genomes approved a higher number of rDNA sites as well as variation in chromosomal rDNA location in L. perenne. A greater instability of F. pratensis-genome-like and L. perenne-genome-like chromosomes in tetraploid hybrids was revealed, indicating gains and losses of rDNA loci, respectively. Our data indicate that the rDNA loci physically mapped on chromosomes 2 and 3 in F. pratensis and on chromosome 3 in L. perenne are useful markers for these chromosomes in intergeneric Festuca × Lolium hybrids.

12

Taxonomy, cytogenetics and phylogenetic relationships in the Lolium-Festuca complex [Poaceae]: I. Lolium - a review

58%

Zwierzykowski Z., Naganowska B.

Fragmenta Floristica et Geobotanica

|

1996

|

tom 41

|

nr 2

13

Intergeneric hybrids of Hordeum vulgare L.x Secale cereale L. regenerated from embryo callus culture

58%

Wojciechowska B., Pudelska H.

Genetica Polonica

|

1992

|

tom 33

|

nr 2

14

Meiosis and fertility in autoallotriploid hybrids between Lolium multiflorum and Festuca pratensis

58%

Naganowska B., Zwierzykowski Z., Zwierzykowska E.

Prace Naukowe Uniwersytetu Śląskiego w Katowicach

|

1998

|

tom 1696

15

Charakterystyka wybranych cech biotechnologicznych hybrydow drozdzy Saccharomyces i Schwanniomyces occidentalis

51%

Dziuba E., Kawa-Rygielska J.

Acta Scientiarum Polonorum. Biotechnologia

|

2003

|

tom 02

|

nr 1-2

67-72

16

Rapid assessment of early embryo development in some wide crosses of cereals

51%

Slusarkiewicz-Jarzina A., Ponitka A., Wojciechowska B., Wozna J.

Journal of Applied Genetics

|

2000

|

tom 41

|

nr 2

17

Mieszance oddalone Triticum aestivum L. jako zrodla odpornosci na choroby klosa

44%

Pilch J., Glowacz E., Kubara-Szpunar L., Gajda Z.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

|

1995

|

tom 194

159-167

18

Charakterystyka niektorych cech mieszancow xTriticosecale Wittmack z Aegilops sp. generacji B2-F1 i F2

37%

Gruszecka D.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

|

1998

|

nr 205-206

163-173

19

Usefulness of some interspecific and intergeneric hybrids T.aestivum L. in breeding for resistance of hexaploid winter wheat

37%

Pilch J., Kubara-Szpunar L., Glowacz E.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

|

1993

|

tom 187

7-12

Ograniczanie wyników

Embryo-callus regenerated hybrids and amphidiploids of barley cultivars x rye inbred lines

Interspecific and intergeneric hybrids of the Lolium - Festuca complex obtained in Poland in the years 1964-1994 and maintained in the collection at the Institute of Plant Genetics in Poznan

Isozymes in Aegilops kotschyi and Ae.biuncialis x Secale cereale hybrids and Ae.kostchyi x S.cereale amphiploids in relation to their parents

Performance of interspecific and intergeneric hybrids of Triticum aestivum L. for wheat improvement. Part I. Performance of winter generations F3-F5 of T.aestivum L. with Triticum [2x,4x], Aegilops [2x] and Elymus [4x] species in respect of some characters of spike

Pollen morphology and two-dimensional patterns of pollen coat and protoplast proteins in Aegilops kotschyi x Secale cereale amphiploids

Chromosome pairing in triploid intergeneric hybrids of Festuca pratensis with Lolium multiflorum, revealed by GISH

Lista mieszańców storczykowatych (Orchidaceae) we florze Polski

Mozliwosci wykorzystania mieszancow miedzygatunkowych i miedzyrodzajowych w hodowli pszenicy jarej

Zarodnikowanie szczepow z rodzaju Saccharomyces i Schwanniomyces oraz produktow ich fuzji

Charakterystyka zbiorów kolekcyjnych pszenżyta jako mieszańca międzyrodzajowego

Variability of ribosomal DNA sites in Festuca pratensis, Lolium perenne, and their intergeneric hybrids, revealed by FISH and GISH

Taxonomy, cytogenetics and phylogenetic relationships in the Lolium-Festuca complex [Poaceae]: I. Lolium - a review

Intergeneric hybrids of Hordeum vulgare L.x Secale cereale L. regenerated from embryo callus culture

Meiosis and fertility in autoallotriploid hybrids between Lolium multiflorum and Festuca pratensis

Charakterystyka wybranych cech biotechnologicznych hybrydow drozdzy Saccharomyces i Schwanniomyces occidentalis

Rapid assessment of early embryo development in some wide crosses of cereals

Mieszance oddalone Triticum aestivum L. jako zrodla odpornosci na choroby klosa

Charakterystyka niektorych cech mieszancow xTriticosecale Wittmack z Aegilops sp. generacji B2-F1 i F2

Usefulness of some interspecific and intergeneric hybrids T.aestivum L. in breeding for resistance of hexaploid winter wheat