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The aim of this study was to determine the impact of diet on the fatty acids profile in European catfish meat. The study material was comprised of fish reared in earthen ponds and fed with natural food and fish fed intensively with artificial feed in a recirculating system. The quality of the fatty acids profile in the meat of the two groups differed. A total of twenty-eight acids were identified in the catfish reared on natural food. The fish fed artificial feed contained an additional two acids – 22:1n-9 and 16:4, which were also detected in the artificial feed. The combined total share of saturated acids, MUFA, and PUFA was similar. However, the share of most of the fatty acids from these groups, including total PUFA n-6 and n-3 and HUFA n-3 and n-6, differed significantly. The catfish fed on artificial feed contained more n-3 acids and fewer n-6 acids. Above all, this was caused by the greater share of 20:5n-3 (eicosapentaenoic, EPA), 22:6n-3 (docosahexaenoic, DHA), and 20:3n-3 and 20:4n-3 acids and the lesser share of 18:3n-3 (a-linolenic, ALA) acid. The meat of the fish from this group also contained fewer 20:4n-6 (arachidonic, AA), 20:3n-6, and 22:5n-6 acids. Additionally, the n-3/n-6 acid ratio was significantly different at 2.31 (pond culture) and 3.93 (intensive culture on artificial feed).
The aim of this study was to describe the development of the swim bladder of pikeperch Sander lucioperca (L.) under conditions of intensive culture and to determine when the pneumatic duct regresses. The development of the swim bladder was observed over a 30-day period of larvae culture in a recirculation system. The swim bladder and the pneumatic duct were clearly visible in histological cross sections of 4-day-old larvae. On day 6 after hatching significant changes occurred in the microscopic structure of the swim bladder, e.g. the gas gland and oval primordia appeared. The pneumatic duct gradually atrophied in the larvae with inflated swim bladders between days 11 and 13, while it remained open until day 24-26 after hatching in fish with noninflated swim bladders. The swim bladders of the latter group were irregular in shape and hyperplasia of the epithelium and connective tissues and the presence of macrophags were noted. Pikeperch larvae with noninflated swim bladders also suffered from lordosis and slower growth rates.
Carp were cultivated in polyculture with grass carp, silver carp and European catfish. The chemical parameters (pH, oxygen, ammonia nitrogen, nitrate nitrogen, nitrite nitrogen, total nitrogen, phosphates, total suspensions, chemical oxygen demand CODMn) of discharged water and four pond layers were measured during the autumn harvest. The study indicated that when the water was discharged the amounts of nitrogen compounds, phosphates and especially total suspension increased from 40 mg dm⁻³ in the first layer to 152 mg dm⁻³ in the last layer and that chemical oxygen demand CODMn increased from 10 mg dm⁻³ in the first layer to 176 mg dm⁻³ in the last layer. The results of both physical and chemical tests on the water discharged from ponds during two periods of the autumn harvest were used to calculate the magnitudes of the loads of organic matter, total suspension and nutrients. The loads of organic matter (expressed as CODMn in an acidic medium), nitrogen, phosphorus and suspensions in the discharged water were determined per ha of area. The highest values were observed in T-type ponds (1,500 specimens ha⁻¹) at an average of 1,214 kg ha⁻¹ and in G3-type ponds (4,000 specimens ha⁻¹ ) with an average of 1,198 kg ha⁻¹ . This data allowed the chemical parameters (kg ha⁻¹) of waters discharged from the ponds in autumn to be compared with the level of CODMn and the amount of nutrients which are introduced to the ponds in spring, as well as to determine the degree of nutrient and organic matter retention in the pond. In the majority of cases nitrogen is fully retained, phosphates are either fully or partially retained and there is an increase in water chemical oxygen demand CODMn especially during the harvest in September.
Monitoring the condition of fish and protecting them from diseases are two of the most important aspects of prevention in intensive fish culture. Nonspecific cellular and humoral defense mechanisms were studied for the first time in pikeperch (Sander lucioperca) grown in an intensive system of culture using two sizes of fish weighing approximately 50 g and 500 g. The respiratory burst activity and potential killing activity of blood phagocytes and pronephric macrophages were measured in each group. The proliferative response of lymphocytes stimulated by mitogen ConA and LPS was also examined in the two groups of fish. The lysozyme and ceruloplasmine activities in the plasma and the total protein with Ig levels in the serum were also determined. These results will be used in controlling the influence of diets and xenobiotics on nonspecific cellular and humoral defense mechanisms and for infectious disease prevention in pikeperch raised in different systems of culture.
Pikeperch culture is a new direction in European aquaculture. There is scant knowledge of pikeperch physiology, especially regarding hematological and biochemical parameters in the blood. This study examines selected hematological parameters in the blood and the biochemical parameters in the plasma of pikeperch from intensive culture. To determine the hematological parameters, blood was drawn from 20 healthy fish, weighing 40-50 g. The red blood cell count (RBC), hematocrit (Ht), hemoglobin (Hb), various leukocyte counts, the total leukocyte level and MCV, MHC and MCHC counts were examined. The AST, ALT, ALP, plasma bilirubin, cholesterol, glucose, urea and total protein level were determined in the blood plasma. The analyses of the results suggest that pikeperch have similar hematological and biochemical parameters to rainbow trout. The results of this preliminary study indicate that fish from this new intensive culture were in good condition without any symptoms of diseases.
The experiment was run on carp individuals in their second year of life. The fish were randomly divided into two groups fed two different isoprotein diets, one with increased fat level (Group I) and the other with increased carbohydrate level (Group II). Cortisol, total protein and protein fraction contents, AspAT and AlAT activities, and body composition and weight increments were monitored. Statistically significant differences between the two experimental groups were found in cortisol content, albumin level, blood AspAT and AlAT activities as well as in dry weight, total protein, and fat contents of the fish body.
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