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Studies concerning the ultrastructure of the periendothelial zone integumentary cells of Asteraceae species are scarce. The aim was to check whether and/or what kinds of integument modifications occur in Onopordum acanthium. Ovule structure was investigated using light microscopy, scanning electron microscopy, transmission electron microscopy and histochemistry. For visualization of calcium oxalate crystals, the polarizing microscopy was used. The periendothelial zone of integument in O. acanthium is well developed and composed of mucilage cells near the integumentary tapetum and large, highly vacuolated cells at the chalaza and therefore they differ from other integumentary cells. The cells of this zone lack starch and protein bodies. Periendothelial zone cells do not have calcium oxalate crystals, in contrast to other integument cells. The disintegration of periendothelial zone cells was observed in a mature ovule. The general ovule structure of O. acanthium is similar to other members of the subfamily Carduoideae, although it is different to “Taraxacum”, “Galinsoga” and “Ratibida” ovule types.
The combination of SEM and autoclave methods gave a clear three-dimensional demonstration of integumental elastic fibres in small densely-haired mammals. The specific organisation of a fine and spongy elastic network was characterised by uniformly thin elastic fibres which were homogeneously distributed between both hair follicle types throughout the whole dermis. All the hair follicles were connected with each other by elastic fibres along their complete intradermal length. The advantage of such a specific elastic dermis construction is that all hair follicles can be moved together and simultaneously along the entire body, so that a better and rapid insulation is achieved after erection of the hair follicles during very low temperatures.
This study deals with the initiation and development of the integument in flowering plants. A classification including types and variations is proposed. The types (dermal, subdermal and dermal-subdermal) have different origins, that is, the integument may develop either from the dermal or subdermal or from both sets of initials. The characters of the variations comprise a complex of the following features: (1) the number of initials, (2) the sequence of their divisions and the mode of cell wall initiation at the first developmental stages, and (3) the participation of the initials in the formation of the various layers. The main evolutionary trends of the integument and ovule as a whole are considered.
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The integument of Cambrian chancelloriids

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Details of the body surface of the chancelloriid Allonnia from the Lower Cambrian Chengjiang biota in southwestern China elucidate the nature of these enigmatic organisms. Rhombically arranged elements, about 30 x 60 ym, are interpreted as representing imbricating platelets, the distal ends of which projected as spinules from the body surface. Comparisons with other chancelloriids suggest that the flexible integument was continuous with the aragonitic sclerites that sit on the surface like cactus spines, and that both were formed by an epidermal epithelium secreting a continuous exo- and endocuticle. In the sclerites, the exocuticle was mineralized; the unmineralized endocuticle and cellular extensions from the epithelium filled the interior of the sclerites. In the flexible integument the epithelium was overlain by endocuticle and unmineralized exocuticle. This structure of soft integument and sclerites is at variance with proposals of poriferan or ascidian affinity of chancelloriids but in accord with a coeloscleritophoran model.
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