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The goal of this study was the assessment of the influence on both insulin and growth hormone levels in lambs blood of blood meal and Wroclaw product - dry brown livex (modified by whey). Experiments were carried out on 30 young Polish "wielkopolska" lowland - sheep - wool type, aged 3-4 months. Livex and blood meal constituted 2% of forage. Blood samples for radioimmunologic examinations (GH and insulin level determination) were taken 1 hour before feeding, directly before feeding and 1.0, 1.5, 2.0, 3.0, 5.0, 6.0 and 8.0 hours afterwards. During the experiment higher levels of insulin and GH were observed in animals fed with livex and forage mixture than in animals fed with blood meal and forage mixture. Livex caused a bigger body mass increase (mean increase 27.3%) in comparison with animals fed with control forage. Although neither livex nor blood meal are hormone preparations, changes in insulin and growth hormone levels were observed.
The aim of the research was to determine whether short-time heart muscle ischemia caused by fast ventricular pacing rate (ventricular tachycardia) provokes insulin and glucose blood level alterations and if so, how long those changes last after return to the physiological heart rate and restoration of proper heart muscle perfusion as well. It was stated that short-lasting tachycardia provoked by fast, forced ventricle rate, with no damage on the cardiac muscle resulted in significant fall of the glucose and insulin blood levels. The glucose returned to its initial level several minutes after heart rate normalisation; however, the insulin drop maintained for more than 10 min.
Male rats of Wistar strain (n = 48) were fed a vitamin A deficient diet for 3 days of adaptation period and then a 10 day experimental period to reduce slightly the body stores of this vitamin. Half of the animals were subjected to physical training and/or oral vitamin A supplementation. Four different doses of supplementation were used – 0, 7.5, 15 and 60 μg/d/rat, which is equivalent to 0, 25, 50 and 200 IU of vitamin A, respectively. Animals from the defined groups ran on a treadmill with a rate of 2.0 m/s for 15 minutes per day for 10 days. After overnight fasting, the rats were sacrificed, and insulin in blood serum and hepatic retinol concentrations were estimated. Daily feed intake and daily body gains were similar in groups of sedentary and physically trained rats. A moderate level of oral vitamin A supplementation (the highest supplemented dose was about 6 x the above recommended NRC level) did not cause any changes in these zootechnical parameters. Oral Vitamin A supplementation resulted in an increase in retinol concentration in the liver (F = 15.2, p < 0.001), but without significant difference between trained and untrained animals. Physical training of rats caused a statistically significant decrease of insulin concentration in blood serum (1.53 ± 0.18 vs. 1.73 ± 0.20). This difference was highly significant (F = 11.1, p < 0.001). Vitamin A supplementation was found not to influence the concentration of this hormone, which is responsible for energy metabolism regulation in the body. Based on estimated parameters, the necessity of vitamin A excessive use in physically trained subjects was not proven.
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