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The present work aimed at investigating diversified organic treatment including sewage sludge on the insecticide activity of enthomopathogenic fungi and nematodes. Crude sludge from biological tannery sewage treatment plant and vermicomposts based on the sludge with added straw and fruit tree leaves were used for the experiment. Organic treatment significantly affected pathogenic properties of enthomopathogenic nematodes naturally present in the soil of the investigated plots. The highest death rate among trap insects was detected in the soil from untreated plots or fertilised by farmyard manure and vermicomposts. Nematodes from examined plots reproduced in varying numbers depending on the treatment applied on individual plots. The lowest number of invasive larvae was obtained for nematodes from plots fertilised by straw vermicompost, (crude)sewage sludge and hair. The applied organic treatment influenced also insecticide activity of the applied nematodes towards G. mellonella larvae. The highest death rate of G. mellonella larvae was observed in the soils treated with organic fertilisers and farmyard manure. Clear decrease in B. bassiana pathogenic properties was detected in soil fertilised with sewage sludge and hair. Chemical sludge and fertilisation with hair caused also a clear decrease in S. carpocapsae pathogenic properties towards G. mellonella larvae. The obtained results reveal unfavourable effects of crude sewage sludge on the pathogenic properties of enthomopathogenic fungi and insects. Vermicomposts obtained from this sludge revealed a positive influence on the microorganisms. This fact may indirectly improve health conditions of plants.
Extensive studies have been carried out to identify plant proteins with insecticidal properties towards insects. The paper describes a test for screening toxicity and growth inhibition of five commercially available proteins such as: bromelain, honey bee venom, two lectins-wheat germ agglutinin (WGA), concanavalin A (Con A) and lysozyme in artificial diets on Sitobion avenae /F./. Grain aphid proved to be sensitive to all tested proteins. Among the tested proteins concanavalin A and wheat germ agglutinin had the highest negative influence on feeding, reduced weight and increased mortality of tested aphids independently from the dose. It suggested that this group of the proteins have an insecticidal activity and is a good candidate for control of the insect pests.
Attempts to obtain a micro-dispersion-based formulation insecticide were made. An aqueous micro-suspension of polystyrene had been used to trap pyrethroid lambda-cyhalothrin. A method of introducing the active substance into the polymer was developed. The biological efficiency of micro-emulsion of lambda-cyhalothrin with polystyrene was investigated.
The Mediterranean flour moth, Ephestia kuehniella Zeller, is a major pest of stored grain products, particularly flour. There have been major concerns over the application of conventional insecticides in stored products, which have strongly demonstrated the need for applying such alternative safe compounds as essential oils. The aim of the present study is to investigate the chemical composition and fumigant toxicity of essential oil from Ziziphora clinopodioides Lam. against the eggs, larvae, and adults of E. kuehniella. All toxicity tests were carried out under laboratory conditions set at 26±1°C and 70% relative humidity (RH). The results of gas chromatography- -mass spectrometry (GC/MS) analysis indicated that the essential oil extracted from the leaves of Z. clinopodioides, is rich in pulegone (49.4%), piperitenone (10.7%), menthone (8.9%), and 1,8-cineol (6.9%). Based on the bioassay results, the LC50 value of the tested oil was estimated to be 54.61 μl · l–1 air for larvae and 1.39 μl · l–1 air for adults. Also, it is shown that increasing the oil concentration resulted in a significant increase in oviposition deterrency as well as a considerable reduction in the egg hatching percentage. These results suggest that Z. clinopodioides oil could be a potential candidate as a fumigant for managing E. kuehniella in stored products.
The objective of current study was to determine the chemical constituents and fumigant toxicity of essential oil isolated by hydro-distillation from dry fruit of bitter fennel (Foeniculum vulgare Miller). The chemical composition of the essential oil was assessed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Constituents of the oil were determined as α-pinene (1.6%) and limonene (3.3%), fenchone (27.3%), estragol (3.9%), and (E)-anethole (61.1%). The fumigant toxicity of the essential oil was tested on larvae of the stored product insect Trogoderma granarium Everts. The mortality of larvae was tested at different concentrations ranging from 31.2 to 531.2 μl/l air and at different exposure times (24 and 48 h). Probit analysis showed that LC50 and LC90 following a 48 h-exposure period for essential oil were 38.4 and 84.6 μl/l, respectively. These results showed that the essential oil from F. vulgare may be applicable to the management of populations of stored-product insects.
Highly active antagonistic actinomycète Streptomyces griseoviridis and entomopathogenic fungus Beauveria bassiana were applied to the soil separately and together (in association) in the laboratory experiments. We assessed survival rate, insecticidal and fungistatic activity of these strains. We also tested the influence of synthetic insecticide Regent 25® (fipronil 25g/l) on investigated parameters. Additionally, insecticidal activity of both strains was compared with insecticidal activity of Regent. It was shown that both strains, especially S. griseoviridis, good survived in soil. Population density of S. griseoviridis in 1he association with B. bassiana increased 2-3 times compared to initial density. Regent considerably reduced population density of S. griseoviridis and B. bassiana. Insecticidal efficiency of S. griseoviridis was comparable with the effect of synthetic incecticide Regent and reached 89.2% and 86.8% respectively. Fungistatic activity towards Fusarium oxysporum showed only S. griseoviridis and it was observed that this activity decreased in time course.
Our research provides novel information concerning the insecticidal activity of Brassica alba mustard oil applied to the intestinal tract via insects’ diet against pests from the order Lepidoptera: Cydia pomonella, Dendrolimus pini, and Spodoptera exigua. The LC50 value of the oil against C. pomonella was 0.422 mg ⋅ ml–1. The LC50 of the plant oil against D. pini was 11.74 mg ⋅ ml–1. The LC50 of the botanical product against S. exigua was 11.66 mg ⋅ ml–1. The plant substance was the most active against C. pomonella in comparison with D. pini and S. exigua. The LC50 values of the oil against D. pini and S. exigua were similar. The plant oil exhibited high insecticidal activity against pests from the order Lepidoptera and may prove to be an effective biopesticide.
Cyanobacterial biofilms serve as food and shelter for benthic invertebrates, such as juvenile insects. Chironomids are often the most widely distributed and abundant insect larvae in freshwater ecosystems. As a consequence of high grazing pressure, effective defence mechanisms can be expected in biofilm-forming organisms. The presence of chemical defence was studied in 12 axenic and monoxenic cyanobacterial species. Flakes of cyanobacterial biofilms were offered to Chironomus riparius (Meigen) over a period of 8 days. Mortality and body-length of the surviving animals were used as indicators for the toxicity of the cyanobacteria and their suitability as food. Toxicity and inhibition of larval growth were found for several cyanobacteria tested. Fischerella sp. (ATCC 43239) was the most active and caused 100% mortality in Chironomus larvae within 24 h. Mortality was also high (87%) for larvae fed with Aphanothece sp. Moderate toxicity (40–60% mortality) was found for Calothrix sp. (PCC 7507), C. braunii Bornet et Flahault, C. thermalis (Schwabe) Hansgirg and a cyanobacterium of the LPP group designated JU 5. Mortality of 7–33% was observed for Calothrix parietina (Nägeli) Thuret, Oscillatoria brevis (Kützing) Gomont, Cylindrospermum sp., Nostoc sp., Calothrix anomala Mitra and a cyanobacterium of the LPP group designated 5 KB. Differences depending on the cyanobacterial food offered were also seen in the lengths of surviving larvae. Fischerella sp. (ATCC 43239) was studied in more detail to chemically characterise the observed insecticidal activity. The insecticidal activity could be extracted with 60% aq. methanol from the fresh biomass and caused 100% mortality in Chironomus. A literature survey was performed on the bioactive compounds so far isolated and characterised from Fischerella and related Stigonematales. It is noticeable that no insecticidal activity has been shown for any of these compounds yet. The newly found insecticidal property of Fischerella may lead to the identification of bioactive compounds which may be important as chemical defence against insect grazers.
Badania nad efektywnością ekonomiczną zastosowanych pestycydów w pszenicy ozimej w latach 2007-2008 wykonano w Boguchwale. Średnie porażenie powierzchni liści przez choroby pszenicy wyniosło odpowiednio 85,2 i 75,8%, a uszkodzenie przez szkodniki - 34,3%. Skuteczność zastosowanych środków grzybobójczych wyniosła od 17,4 do 89,9%, a insektycydów od 73,4 do 90,4%. Nadwyżka produkcji wahała się od 217 do 1393 PLN·ha-1. Wskaźnik pokrycia kosztów wyniósł od 1,4 do 5,1, a wskaźnik opłacalności zabiegów od 1,0 do 7.4. Procentowy wskaźnik kosztów wahał się od 1,1 do 7,4.
The research on results of chemical control of thrips (Thysanoptera) took place in Krzeczowice near Przeworsk in 2008–2009. The seed dressings used were: Gaucho 600 FS (imidacloprid), Mesurol 500 FS (methiocarb) and Poncho 600 FS (clothianidin). They effectively protected maize plants against thrips feeding for a period of about 10 weeks from sowing. Chemical control of European corn borer (Ostrinia nubilalis Hbn.), performed in the first and second decade of July using formulations Karate Zeon 050 CS (lambda-cyhalothrin) and Proteus 110 OD (thiacloprid + deltamethrin), also effectively limited the number of thrips in the period of their mass occurrence. The highest effectiveness was observed following two applications of tested insecticides.
Responses (knock-down and mortality) of large pine weevil (Hylobius abietis L.) beetles to insecticides of different mechanism of action such as neonicotinoids (clotianidin, acetamiprid) acting on nicotinic receptors of acetylcholine (ACh), pyrethroids (deltamethrin, α-cypermethrin) blocking sodium channels in nerve cell membranes, organophosphates (chlorpiryfos) and carbamates (carbosulfan) both inhibiting acetylcholinesterase (AChE) activity and fenylpirazoles (fipronil) interfering with γ-butyric acid (GABA) receptors were studied. Taking into account speed of insect decay the order of examined groups of substances may be established as follows: first place is occupied by insecticides interferring with GABA receptors, second place by insecticides blocking AChE activity, and third place in parallel insecticides blocking sodium channels and those acting on nicotinic receptors of ACh.
The studies on a population and economic effectiveness of insecticides in control of aphids in spring barley were conducted in Boguchwała in the years 2010–2012. The aphid population reached its maximum in the third decade of May or the first half of June, at the flag leaf stage (BBCH 37–41) in the spring barley. In 2012, the aphids were very numerous, reaching an average number of 10.5 individuals per stalk in spring barley. In 2011–2012, all formulations used in the experiments were characterised by a high effectiveness in aphids control in the spring barley. The increase in the barley yield ranged from 4.0 to 9.6 dt/ha. The value of saved barley yield ranged from 304 to 799 PLN per ha. The contribution ratio ranged from 3.4 to 9.1, 6.6 on average. The profitability ratio for barley treatment was 1.1. The percentage cost ratio ranged from 1.5 to 1.6.
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