Molecular probes were generated to study the expression of tyrosine hydroxylase (TH), choline acetyltrans- ferase (ChAT), galanin (GAL), substance P (SP) and calcitonin gene-related peptide (CGRP) in the peripheral nervous system of the pig with in situ hybridization. Application of the probes to the sections from the inferior mesenteric ganglion (IMG) in control animals revealed numerous neurons displaying a hybridization signal for TH, while less numerous nerve cells exhibited the hybridization signal for GAL and ChAT. In the spinal ganglia L1-L3 the hybridization signal for SP and CGRP was detected in the vast majority of neurons, while the hybridization signal for ChAT was found in much smaller population of ganglionic nerve cells. After injection of retrograde tracer Fast Blue (FB) into the porcine ovary and subsequent ovariectomy, FB-positive neurons were detected in the ipsilateral IMG and spinal ganglia L1-L3. Application of a molecular probe for GAL to sections from IMG in ovariectomized animals revealed that the majority of FB-positive neurons displayed the hybridization signal what may suggest increased de-novo synthesis of the peptide in the injured neurons.
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