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Toxicity of four types of industrial wastewater, treated by Fenton’s reagent, was analyzed. Toxicity was measured using bioluminescent bacteria Vibrio fischeri NRRL B-11177. Application of Fenton’s reagent was a efficient method for treatment of this wastewater. Nevertheless, high efficiency of organic components degradation was not always followed by reduction of toxicity to a very low level. In all cases, in order to achieve total reduction of toxicity, it was necessary to increase both the H2 O2 dose and reaction time. It was concluded that optimization of Fenton’s reaction should be performed assuming toxicity changes in the examined wastewater. Reduction in toxicity should be a critical measure of the success of this method. Such an approach should be general practice in relation to all hazardous industrial wastewater.
The process of pesticide (gamma -HCH and inactive isomers alpha- and beta -HCH, DDT, DMDT, fenitrothion and chlorfenvinphos) removal from industrial wastewater using Fenton's reaction has been analysed. Most of the pesticides were completely degraded at H2O2 Concentration of 2.5 g/dm(3); however, only at 5 g/dm(3) were all the examined pesticides completely removed and the results repeatable. The most effective process was for fenitrothion (from 98.5 to 100%) and chlorfenvinphos (from 97.1 to 100%), whereas organochlorine pesticides were removed with efficiency of more than 90%. The optimum ratio of [Fe2+] to [H2O2] was from 1:3 to 1:2 while the optimum pH was from 3.0 to 3.5. Tests based upon a bioluminescent bacteria Vibrio fischeri NRRL B-11177, at optimized Fenton's reaction parameters, show the reduction of toxicity to non-toxic levels.
In order to upgrade the quality of anaerobically treated effluent to a level recommended for irrigation, integration of a UASB reactor with UV and AOPs (advanced oxidation processes) (Ozone, H2O2/UV, Fenton, and photo-Fenton) could be a better option for almost complete colour, COD removal, and disinfection of pathogens. High efficiency of the UASB can be maintained by proper process conditions, including temperature, sludge age, pH, hydraulic retention time, and gas-liquid-solid separator (GLSS) design. A fraction of the COD and colour is usually non-biodegradable and renders difficulty for anaerobic digestion. AOPs degrade the organic molecules and converting completely the organic compounds to non-toxic components such as CO2 and/or water. As far as disinfection is concerned, advanced oxidation processes are proved to be extremely effective in killing pathogens (total coliform, fecal coliform, fecal streptococci, salmonella, and E. coli) due to their strong oxidative characters. Although AOPs effectively accomplish pathogen elimination, re-growth of pathogenic microorganisms can take place in the treated effluent. Re-growth potential of pathogens provides helpful information about the quality of the treated water, which is very important in all possible reuse options. The combined application of AOPs with anaerobic treatment minimizes the chances of regrowth due to irreparable damage to nucleic acid. This review paper focuses primarily on the process conditions and treatment efficiency for UASB treatment systems, and to evaluate the advanced oxidation processes (AOPs) as an option for post treatment.
The bacterial degradation of phenol by Alcaligenes xylosoxidans and Pseudomonas putida was studied in a laboratory-scale stirred tank bioreactor. Several kinetic experiments were employed to obtain values of kinetic parameters by applying nonlinear regression methods to differential kinetic models and integrated experimental data. The extra sum of squares for statistical tests of reliability of proposed kinetic models was attached. The values of kinetic parameters based on the Haldane substrate inhibition model for both strains were: μmax=0.347 h-1, Ks=21.5 mg/1 and K1=707 mg/1 for Alcaligenes and μmax=1.988 h-1, Ks=15.92 mg/1 and K1=197.7 mg/1 for Pseudomonas. The measured yield coefficients YX/s were in agreement with values commonly published in literature (0.3-0.8). Degradation rates up to 0.5 g/(l- h) were obtained.
We assessed the bioflocculant production potentials of a consortium of two marine bacterial species belonging to the Oceanobacillus and Halobacillus genera, isolated from sediment samples of Algoa Bay in the Eastern Cape Province of South Africa. Cell free culture broth of the consortium had a flocculating activity of 98.3%, which is higher than those of pure cultures of the individual species. The purified bioflocculant was more efficient (optimum dose 0.2 mg·ml⁻¹) in the flocculation of kaolin suspension (4 g·l⁻¹) compared to polyelectrolyte (optimum dose 0.3 mg·ml⁻¹) and alum (optimum dose 1 mg·ml⁻¹), which are both commercially available coagulants. A neutral pH condition and the presence of Ca²⁺ as cation resulted in optimum activity of the bioflocculant. Also, the purified bioflocculant removed chemical oxygen demand (COD) in brewery wastewater, dairy wastewater, and river water at efficiencies of 99.7, 99.9, and 63.5%, respectively, and also reduced their turbidity by 93.9, 88.3, and 98.6%, respectively. Composition analysis revealed the bioflocculant to be mainly polysaccharide with an amorphous-crystal-like structure. FTIR spectra revealed the presence of carboxyl, hydroxyl, and amino groups in its thermo-stability test, suggesting a thermostable bioflocculant.
The removal of toxic cadmium(II ) and lead(II ) from aqueous solutions was investigated using calcite, which is inexpensive and widespread over the globe, as the effective inorganic adsorbent. The experimental data of the removal equilibrium were correlated by either the Langmuir or Freundlich equations. Results indicate that the Langmuir model gave a better fit to the experimental data than the Freundlich equation. Maximum adsorption capacities were determined as 18.52 mg/g Cd and 19.92 mg/g Pb for natural calcite at 25°C, respectively.
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Wastewater treatment is becoming ever more critical due to diminishing water resources, increasing wastewater disposal costs, and stricter discharge regulations that have lowered permissible contaminant levels in waste streams. The ultimate goal of wastewater management is the protection of the environment in a manner commensurate with public health and socio-economic concerns. The aim of our study is to use natural occurring plant (Pistia stratiotes) to reduce the chemical oxygen demand and color from the industrial waste water. It was found that 120 mg/l of Chemical oxygen demand and 85 mg/l of color reduction was observed with Pistia stratiotes.
Activated Sludge Model No. 1 (ASM1) was used in the modeling of an activated sludge system treating effluents from an oil refinery. The measurements of the diurnal variation in wastewater flow and composition at the wastewater treatment plant inlet and outlet were carried out. The calibrated model predicting the influence of changes in the wastewater composition and the operational parameters on the effluent wastewater quality and the related operational costs is available. A calibration technique based on the heuristic method was applied. The model was used to analyze the influence of changes in wastewater flow and composition as well as different aeration systems on the process performance. The results of dynamic simulations indicate that the verified dynamic mathematical model is a useful supporting tool for optimizing operational parameter values and operational costs. The presented results are for illustration purposes only and are not intended as instructions for the operation of a wastewater treatment plant.
Wastewater treatment is becoming ever more critical due to diminishing water resources, increasing wastewater disposal costs, and stricter discharge regulations that have lowered permissible contaminant levels in waste streams. The ultimate goal of wastewater management is the protection of the environment in a manner commensurate with public health and socio-economic concerns. The aim of our study is to use natural occurring polymeric coagulant to reduce the chemical oxygen demand and color from the industrial waste water. It was found that 83 % of Chemical oxygen demand and 90 % of color reduction was observed with chitosan.
Our aim was to investigate the biosorption of Pb²⁺, Hg²⁺, Cd²⁺ from aqueous solution by Aspergillus terreus (both free and immobilized on loofa sponge discs). Our results show that the adsorption capacity of fungal biomass on loofa sponge (FBLS) is superior to free fungal biomass (FFB). The adsorption selectivity by FBLS was in the order Pb²⁺>Hg²⁺>Cd²⁺. The maximum metal ions adsorbed was 247.2,37.7, 23.8 mg/g FBLS for Pb²⁺, Hg²⁺ and Cd²⁺, respectively. Metal uptake by FBLS was affected by the pH of the metal solution, but independent of temperature (10-50°C). The Langmuir model was more suitable than the Freundlich model to describe the biosorption process of FBLS. The regenerated FBLS was found to be effective for repeated use for five cycles without significant loss in adsorption capacity. This research demonstrates that FBLS possesses excellent capacity for Pb²⁺ biosorption from aqueous solution and industrial wastewaters.
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Removing lead from Iranian industrial wastewater

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Metals and chemicals have been increased in industrial processes which they contain a high level of toxic heavy metals and cause a lot of disadvantages for the environment and human health .Biosorption of Pb (П) ions has been studied from aqueous solutions in a batch system by using a bacterial strain isolated from petrochemical wastewaters. Strain 8-I was selected to study the impact of different factors on removal rate. According to morphological, physiological and biochemical characterizations of the strain and in comparison with other studies the strain was tentatively identified as Bacillus sp strain 8-I. The maximum Lead biosorption capacity of 8-I isolate was determined to be 41.58 % at pH 4.0 with 80 mg/l concentration in 48 hours equilibrium time. The comparison between the biosorption capacity of live (45.50 mg/g), heat inactivated (30.23 mg/g) and NaN3 pretreated biomass (26.86 mg/g) were indicated that the ability of live biomass for both of active and passive uptake of lead.
The results of comparative experiments on aerobic digestion of excessive activated sludge using hydrogen,peroxide and Fenton’s reagent are described. Fenton’s reagent was found to have a higher oxidation potential and, as a consequence, higher efficiency in a digestion process in comparison to hydrogen peroxide. Moreover, Fenton’s reagent was shown to improve sedimentation properties of sludge and to decrease soluble COD more efficiently than H2O2. The main advantage of the Fenton’s reagent application was the fact that oxidation processes took place even though Fenton’s reagent was no longer added. On the other hand, the apparent disadvantage of its application is the formation of additional chemical sediments and possible decomposition of sludge flocs as a result of overdosage of reagents and, consequently, an increase in turbidity of supernatant liquid and some difficulties with sludge dewatering.
Denitriiication using real wastewaters from chemical production was studied in lab-scale reactors. The wastewater streams contained as main compounds vinyl acetate, acetaldehyde and methanol. The highest denitrification rates were estimated in the wastewater with vinyl acetate. The effect of the tested substrates on endogenous metabolism and nitrification inhibition is discussed.
The effect of intensive irrigation with wastewater on the zinc (Zn) and cadmium (Cd) concentration in soil solution was investigated. The experiment was parallel conducted on two soil profiles. The soil microcosms were watered with purified wastewater and purified wastewater with an the addition of zinc and cadmium. The results indicate clearly that neither intensive overhead irrigation of the soil nor its flooding with these wastewater and exceeded sorptive capacity. The application of treated wastewater and wastewater with heavy metals addition did not appear to pose a threat to the natural environment. In all the cases under analysis, zinc and cadmium concentrations in the soil solution were several-fold lower than the permissible levels.
Ozonation was carried out for decolorization and COD removal of raw and anaerobically treated UASB (upflow anaerobic sludge blanket) combined industrial (mainly textile) effluent in a lab-scale bubble column reactor. Ozonation of anaerobic bioprocess effluent at a dose of 300 mg/h for 10 min resulted in 81% color and 75% COD removal (100 mgO3/80 mg COD) while for raw wastewater 25 min ozonation furnished 51% color and 67% COD removal (250 mgO₃/345 mg COD). Optimal process conditions for biotreated effluent (pH = 8 and temperature = 25°C) resulted in 100% color and 96% COD removal for 10 min ozonation (100 mgO₃/104 mg COD). Electrical energy comparison demonstrated that post ozonation required less electrical energy, 16 and 2.9 times as compared to pre ozonation.
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