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1
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Eosinophilic airway inflammation in chronic obstructive pulmonary disease and asthma

100%
Gorska K., Krenke R., Korczynski P., Kosciuch J., Domagala-Kulawik J., Chazan R.
Journal of Physiology and Pharmacology. Supplement
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2008
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tom 59
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nr 6
261-270
2
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Effects of homogenization of induced sputum by dithiothreitol on polymorphonuclear cells

84%
Van Overveld F. J., Demkow U., Gorecka D., Skopinska-Rozewska E., De Backer W. A., Zielinski J.
Journal of Physiology and Pharmacology. Supplement
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2005
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tom 56
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nr 4
143-154
Induced sputum represents a useful and non-invasive tool to isolate different cells from the airways. Complete homogenization of sputum is important for dispersion of cells and is usually achieved by use of dithiothreitol (DTT). However, it is not known if DTT will influence the viability and functionality of cells obtained by induced sputum. In the present study, induced sputum was processed by DTT or by PBS treatment. The obtained neutrophils were compared with neutrophils obtained from peripheral blood and from bronchoalveolar lavage fluid (BAL). These isolated neutrophils were treated in a similar way as the sputum neutrophils with DTT or PBS. All isolated cells were used for chemiluminescence tests and for the measurement of elastase and myeloperoxidase release after stimulation with fMLP. The results showed that the maximum chemiluminescence response was always significantly lower after DTT treatment: blood, 16.68 ±1.89 vs. 2.62 ±0.43 mV, P<0.0001; sputum, 2.96 ±0.30 vs. 1.09 ±0.01 mV, P<0.01; BAL, 25.47 ±0.88 vs. 8.22±0.20 mV, P<0.0001. Both spontaneous and fMLP-induced release of elastase and myeloperoxidase (MPO) was in most cases enhanced after DTT-treatment (P-values range from 0.24 to <0.01). We conclude that the use of DTT to homogenize sputum for dispersion of cells is harmful to cell functions and these cells are hampered for the evaluation of their normal functional characteristics.
3
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Induced sputum metalloproteinases and their inhibitors in relation to exhaled nitrogen oxide and sputum nitric oxides and other inflammatory cytokines in patients with chronic obstructive pulmonary disease

84%
Ziora D., Dworniczak S., Kozielski J.
Journal of Physiology and Pharmacology. Supplement
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2008
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tom 59
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nr 6
809-817
4
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Induced sputum in patients with interstitial lung disease: a non-invasive surrogate for certain parameters in bronchoalveolar lavage fluid

84%
Sobiecka M., Kus J., Demkow U., Filewska M., Jozwik A., Radwan-Rohrenschef P., Chorostowska-Wynimko J.
Journal of Physiology and Pharmacology. Supplement
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2008
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tom 59
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nr 6
645-657
5
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Induced sputum eosinophils, bronchial reactivity, and cough sensitivity in subjects with allergic rhinitis

67%
Tatar M., Petriskova J., Zucha J., Pecova R., Hutka Z., Raffajova J., Brozmanova M.
Journal of Physiology and Pharmacology. Supplement
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2005
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tom 56
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nr 4
227-236
Some patients with allergic rhinitis and no clinical evidence of asthma exhibit bronchial hyperresponsiveness. In the present study, induced sputum and acetylcholine and capsaicin challenges were assessed in four groups of adult subjects: allergic rhinitis (AR), allergic rhinitis with lower airway symptoms (ARLA), mild stable asthma (BA), and healthy volunteers (C) to correlate lower airway inflammatory markers with bronchial and cough reactivity. Patients with AR (n = 13) and ARLA (n = 11) did not take any anti-inflammatory drugs. Those with BA (n = 9) used inhaled corticosteroids and C (n = 10) were respiratory symptoms free. The patients underwent capsaicin cough challenge and sputum induction with hypertonic saline during the first visit, and acetylcholine bronchial challenge on a separate day. We found that the percentage of eosinophils in induced sputum was significantly higher in patients with AR, ARLA, and BA than in C 14.5 ±1.8(SE) vs. 13.5 ±2.9 vs. 13.9 ±4.0 vs. 3.6 ±0.8 %, respectively (P=0.012). In contrast, acetylcholine PD20 in patients with AR, ARLA, and BA was significantly lower than in C 5.6 ±0.9 vs. 4.1 ±0.4 vs. 2.8 ±0.4 vs. 12.9 ±2.7 mg, respectively (P=0.0001). Neither the eosinophil percentage nor PD20, nor cough sensitivity appreciably differed across the patients groups. Sputum eosinophils correlated significantly with the acetylcholine PD20 (r=0.37, P=0.016). We conclude that eosinophilic inflammation of lower airways and increased bronchial reactivity were present in adult patients with allergic rhinitis.
6
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Comparison of cellular and biochemical markers of airway inflammation in patients with mild-to-moderate asthma and chronic obstructive pulmonary disease: an induced sputum and bronchoalveolar lavage fluid study

67%
Gorska K., Krenke R., Domagala-Kulawik J., Korczynski P., Nejman-Gryz P., Kosciuch J., Hildebrand K., Chazan R.
Journal of Physiology and Pharmacology. Supplement
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2008
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tom 59
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nr 6
271-283
7
Content available remote

Comparison of induced sputum and bronchoalveolar lavage fluid cell profile during the treatment of pulmonary sarcoidosis

67%
Mroz R. M., Korniluk M., Stasiak-Barmuta A., Chyczewska E.
Journal of Physiology and Pharmacology. Supplement
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2007
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tom 58
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nr 5
8
Content available remote

Correlation of exhaled nitric oxide with nitrogen oxides and selected cytokines in induced sputum of chronic obstructive pulmonary disease patients

67%
Ziora D., Dworniczak S., Kaczmarczyk G., Jastrzebski D., Krzywiecki A., Kozielski J.
Journal of Physiology and Pharmacology. Supplement
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2007
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tom 58
|
nr 5
9

Comparison of leukocyte populations from bronchoalveolar lavage and induced sputum in the avaluation of cellular composition and nitric oxide production in patients with bronchial asthma

67%
Bienkowska-Haba M., Cembrzynska-Nowak M., Liebhart J., Dobek R., Liebhart E., Siemieniec I., Panaszek B., Obojski A., Malolepszy J.
Archivum Immunologiae et Therapiae Experimentalis
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2002
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tom 50
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nr 1
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