Wyniki wyszukiwania

1

An investigation of the date of sooty blotch primary infection and duration of incubation period for selected apple cultivars

100%

Grabowski M., Wrona B.

Folia Horticulturae

|

2004

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tom 16

|

nr 1

73-77

2

Effect of temperature on embryonic development of asp [Aspius aspius L.]

100%

Kujawa R., Mamcarz A., Kucharczyk D.

Polskie Archiwum Hydrobiologii

|

1997

|

tom 44

|

nr 1-2

3

Partial characterization and optimization of production of extracellular alpha-amylase from Bacillus subtilis isolated from culturable cow dung microflora

100%

Swain M. R., Kar S., Padmaja G., Ray R. C.

Polish Journal of Microbiology

|

2006

|

tom 55

|

nr 4

Studies of α-amylase production by Bacillus subtilis (CM3) isolated earlier from cow dung microflora, were carried out. The optimum temperature, pH and incubation period for amylase production were 50-70°C, 5.0-9.0 and 36 h, respectively. Enzyme secretion was very similar in the presence of any of the carbon sources tested (soluble starch, potato starch, cassava starch, wheat flour, glucose, fructose, etc.). Yeast extract and ammonium acetate (1%) as nitrogen sources gave higher yield compared to other nitrogen sources (peptone, malt extract, casein, asparagine, glycine, beef extract), whereas ammonium chloride, ammonium sulphate and urea inhibited the enzyme activity. Addition of Ca⁺² (10-40 mM) to the culture medium did not result in further improvement of enzyme production, whereas the addition of surfactants (Tween 20, Tween 40, Tween 80, and sodium lauryl sulphate) at 0.02% resulted in 2-15% increase in enzyme production. There were no significant variations in enzyme yield between shaked-flask and laboratory fermentor cultures. The purified enzyme is in two forms with molecular mass of 18.0 ± 1 and 43.0 ± 1 kDa in native SDS-PAGE.

4

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O rozwoju bulwy ziemniaka

80%

Rykaczewska K.

Ziemniak Polski

|

1994

|

nr 4

16-19

5

Extracellular xylanase production by Fusarium species in solid state fermentation

80%

Arabi M. I. E., Bakri Y., Jawhar M.

Polish Journal of Microbiology

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2011

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tom 60

|

nr 3

Fusarium sp. has been shown to be a promising organism for enhanced production of xylanases. In the present study, xylanase production by 21 Fusarium sp. isolates (8 Fusarium culmorum, 4 Fusarium solani, 6 Fusarium verticillioides and 3 Fusarium equiseti) was evaluated under solid state fermentation (SSF). The fungal isolate Fusarium solani SYRN7 was the best xylanase producer among the tested isolates. The effects of some agriculture wastes (like wheat straw, wheat bran, beet pulp and cotton seed cake) and incubation period on xylanase production by F.solani were optimized. High xylanase production (1465.8 U/g) was observed in wheat bran after 96 h of incubation. Optimum pH and temperature for xylanase activity were found to be 5 and 50°C, respectively.

6

Changes in the number of Fusarium propagules introduced to soil

80%

Kurek E., Jaroszuk J.

Polish Journal of Soil Science

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1997

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tom 30

|

nr 1

7

Production and partial characterization of high molecular weight extracellular alpha-amylase from Thermoactinomyces vulgaris isolated from Egyptian soil

61%

Abou Dobara M., El-Sayed A., El-Fallal A., Omar N.

Polish Journal of Microbiology

|

2011

|

tom 60

|

nr 1

Optimizing production of α-amylase production by Thermoactinomyces vulgaris isolated from Egyptian soil was studied. The optimum incubation period, temperature and initial pH of medium for organism growth and enzyme yield were around 24 h, 55°C and 7.0, respectively. Maximum α-amylase activity was observed in a medium containing starch as carbon source. The other tested carbohydrates (cellulose, glucose, galactose, xylose, arabinose, lactose and maltose) inhibited the enzyme production. Adding tryptone as a nitrogen source exhibited a maximum activity of α-amylase. Bactopeptone and yeast extract gave also high activity comparing to the other nitrogen sources (NH₄Cl, NH₄NO₃, NaNO₃, KNO₃, CH₃CO2 NH₄). Electrophoresis profile of the produced two α-amylase isozymes indicated that the same pattern at about 135145 kDa under different conditions. The optimum pH and temperature of the enzyme activity were 8.0 and 60°C, respectively and enzyme was stable at 50°C over 6 hours. The enzyme was significantly inhibited by the addition of metal ions (Na⁺, Co²⁺ and Ca²⁺) whereas Cl⁻ seemed to act as activator. The enzyme was not affected by 0.1 mM EDTA while higher concentration (10 mM EDTA) totally inactivated the enzyme.

8

Wplyw temperatury i okresu inkubacji na tworzenie toksycznych metabolitow przez Alternaria radicina i A. alternata

51%

Tylkowska K., Grabarkiewicz-Szczesna J., Szopinska D., Dorna H., Solfrizzo M., De G. A.

Acta Agrobotanica

|

2005

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tom 58

|

nr 2

7-17

The production of toxic metabolites by four isolates of Alternaria radicina and two isolates of A. alternata in rice grains and carrot discs at 1, 10 and 20ºC was investigated. Incubation lasted 21 and 35 days or 14 and 28 days for rice grains and carrot discs, respectively. Accumulation of toxins in inoculated carrot roots stored for 24 weeks and in inoculated dried carrots stored for 48 weeks was also determined. It was found that A. radicina produced radicinin (RAD) and epi-radicinol (epi-ROH), whereas tenuazonic acid (TeA), altertoxin I (ATX I), alternariol (AOH) and alternariol methyl ether (AME) were produced by A. alternata. Although the isolates tested were capable of producing toxins in rice grains at 1ºC, none of them was detected in carrot discs. Accumulation of epi-ROH was observed in carrot roots stored for 24 weeks, whereas decreased amounts of RAD and epi-ROH were observed in dried carrots stored for 48 weeks. No A. alternata toxins were detected in stored carrot roots, whereas trace amounts of AOH were recorded in dried carrots after 32 and 48 weeks of storage.

Ograniczanie wyników

An investigation of the date of sooty blotch primary infection and duration of incubation period for selected apple cultivars

Effect of temperature on embryonic development of asp [Aspius aspius L.]

Partial characterization and optimization of production of extracellular alpha-amylase from Bacillus subtilis isolated from culturable cow dung microflora

O rozwoju bulwy ziemniaka

Extracellular xylanase production by Fusarium species in solid state fermentation

Changes in the number of Fusarium propagules introduced to soil

Production and partial characterization of high molecular weight extracellular alpha-amylase from Thermoactinomyces vulgaris isolated from Egyptian soil

Wplyw temperatury i okresu inkubacji na tworzenie toksycznych metabolitow przez Alternaria radicina i A. alternata