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Improvement of in vitro propagation and acclimation of Helichrysum arenarium L. Moench

100%

Figas A., Tomaszewska-Sowa M., Sawilska A., Keutgen A. J.

Acta Scientiarum Polonorum. Hortorum Cultus

2016

tom 15

nr 4

Explants of apical buds of sandy everlasting Helichrysum arenarium L. Moench sterilized with calcium hypochlorite – Ca(OCl₂) were placed onto Murashige- -Skoog (MS) growth medium enriched with 1 mg∙dm⁻³ KIN (kinetin) in two consecutive passages. To optimize the procedure of in vitro micropropagation, the axillary shoots of Helichrysum arenarium L. Moench were transferred during the third passage onto 9 different combinations of MS medium without plant growth regulators (PGRs-free medium), with different concentrations of KIN (1.0, 3.0, 4.0, 5.0 mg∙dm⁻³) and KIN (1.0, 3.0, 4.0, 5.0 mg∙dm⁻³) with IAA (indole-3-acetic acid) (0.5 mg dm⁻³). The highest mean number of shoots (24.7) was observed on MS medium with 5 mg∙dm⁻³ KIN and 0.5 mg∙dm⁻³ IAA. The achieved branched shoots were rooted and acclimatized. Rhizogenesis was intensified by the presence of growth hormones: 0.5 mg∙dm⁻³ IAA or 0.5 mg∙dm⁻³ IBA (indole-3-butyric acid). At the stage of acclimation of plantlets the application of a water solution of MS salts (25%) for watering the plants increased the efficacy of plant acclimation from 56 to 75%.

Ograniczanie wyników

1 Acta Scientiarum Polonorum. Hortorum Cultus

1 Figas A.

1 Keutgen A. J.

1 Sawilska A.

1 Tomaszewska-Sowa M.

1 2016

Wyniki wyszukiwania

Improvement of in vitro propagation and acclimation of Helichrysum arenarium L. Moench