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Simple verification of in vitro - grown clones of the genus Drosera L. using its molecular markers

100%

Fratrikova M., Bauer M., Jopcik M., Libantova J.

Acta Scientiarum Polonorum. Hortorum Cultus

2018

tom 17

nr 1

Drosera L. is a genus of carnivorous plants that comprises approximately 250 species, although this number is probably not complete. Some of these taxa exhibit only small differences in morphological traits that can be partly influenced if the taxa are propagated in vitro. Here, we focus on the verification of putative clones of Drosera spathulata Labill., Drosera rotundifolia L. and Drosera binata var. Dichotoma species cultivated in vitro using molecular markers covering the internal transcribed spacer (ITS) region of 45S ribosomal DNA (rDNA). Following the polymerase chain reaction (PCR) amplification of ~360-bp DNA fragments and sequencing, the sequences were aligned with corresponding sequences in the National Center for Biotechnology Information (NCBI) database. In addition, each of tested PCR amplicons had a specific restriction profile that predominantly enables the differentiation of D. rotundifolia and D. spathulata; the shape of the leaves does not have to be a clear morphologically distinguishable trait.

The influence of biopreparations and synthetic fungicides on growth on micorrhizal fungus Oidiodendron sp. in vitro

80%

Marjanska-Cichon B., Sapieha-Waszkiewicz A., Mietkiewski R.

Acta Scientiarum Polonorum. Hortorum Cultus

2005

tom 04

nr 2

The influence of biopreparations Bioczos SL, Biosept 33 SL, Biochikol 020 PC, Propolis and synthetic fungicides Euparen Multi 50 WG, Horizon 250 EW and Teldor 500 SC was examined on the growth of micorrhizal fungus Oidiodendron sp. Fungicides were added to PDA medium at the following concentrations: recommended dose, 5-times lower than the recommended and 5-times higher than the recommended. The synthetic fungicides were more toxic to Oidiodendron sp. than biopreparations. On the medium containing Horizon 250 EW fungus did not growth. The greather part of biopreparations inhibited growth of Oidiodendron sp. but only Bioczos SL and Propolis in dose 5-times higher than the recommended, toataly inhibited growth of fungus colonies.

Ograniczanie wyników

2 Acta Scientiarum Polonorum. Hortorum Cultus

1 Bauer M.

1 Fratrikova M.

1 Jopcik M.

1 Libantova J.

1 Marjanska-Cichon B.

1 Mietkiewski R.

1 Sapieha-Waszkiewicz A.

1 2018

1 2005

Wyniki wyszukiwania

Simple verification of in vitro - grown clones of the genus Drosera L. using its molecular markers

The influence of biopreparations and synthetic fungicides on growth on micorrhizal fungus Oidiodendron sp. in vitro