The provenance of α-amylase from intestines of adult Ascaris suum was investigated by Ouchterlony method using sera of guinea pigs vaccinated with the enzyme protein extracted from the worm intestine or pig pancreas. No cross-reactivity was observed between sera raised against pig α-amylase and A. suum enzyme. The results support suggestions that α-amylase present in A. suum intestine is produced by the nematode and not required from the host intestine.
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