Four genotypes of Cucumis sativus and Cucumis melo were used to make embryo culture. Embryos were excised from unripe fruits 1, 2 and 3 weeks after self-pollination. MS (Murashige and Skoog, 1962) basal medium was supplemented with ascorbic acid, growth regulators and 2% or 3% sucrose. Higher regeneration was achieved on medium with lower concentration of sucrose and phytohormones. Plants were regenerated from immature embryos isolated 1 week after self-pollination for C. sativus (Sm-6514) and 2 weeks after self-pollination for C. melo.
In vitro somatic embryogenesis and organogenesis (shoot and root formation) were observed when immature zygotic embryos of the sunflower cultivars Frankasol, Printasol, Lech and Wielkopolski were cultured on JME medium containing high sucrose concentration (350 mM). Of four tested cultivars, Frankasol showed the highest rate of somatic embryogenesis, and Printasol the highest rate of organogenesis. The remaining two cultivars responded slightly.
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