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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Identification of relaxation modulus of viscoelastic materials from non-ideal ramp-test histories – problem and method

100%
Stankiewicz A.
Teka Komisji Motoryzacji i Energetyki Rolnictwa
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2013
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tom 13
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nr 1
2

Application of flow cytometer for molecular identification of Bacillus anthracis

100%
Michalski A. J., Bartoszcze M. A., Zabrocka L. B., Szymajda U.
Żywienie Człowieka i Metabolizm
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2007
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tom 34
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nr 3-4
Flow cytometry is a method of identification biological agents that has various applications. It has been applied for identifying many types of antigens in various materials, including environmental samples. Recently it has been noticed that this method could be used for molecular detection of biological agents. The purpose of this work was to apply flow cytometry with nested-PCR for the molecular identification of B. anthracis. Paramagnetic streptavidin-coated beads were used to capture the resulting fluorophore-labeled sequences. The results show that flow cytometry can be successfully used to detect specific fluorescein- dUTP and a biotin marked sequences.
3
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A universal method for the identification of genes encoding amatoxins and phallotoxins in poisonous mushrooms

86%
Woloszyn A., Kotlowski R.
Roczniki Państwowego Zakładu Higieny
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2017
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tom 68
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nr 3
Background. As the currently known diagnostic DNA targets amplified in the PCR assays for detection of poisonous mushrooms have their counterparts in edible species, there is a need to design PCR primers specific to the genes encoding amanitins and phallotoxins, which occur only in poisonous mushrooms. Objective. The aim of the study was testing of PCR-based method for detection of all genes encoding hepatotoxic cyclic peptides - amanitins and phallotoxins present in the most dangerous poisonous mushrooms. Material and Methods. Degenerate primers in the PCR were designed on the basis of amanitins (n=13) and phallotoxins (n=5) genes in 18 species of poisonous mushrooms deposited to Genbank of the National Center for Biotechnology Information. Results. The specificity of the PCR assays was confirmed against 9 species of edible mushrooms, death cap - Amanita phalloides and panther cap - Amanita pantherina. Conclusions. Designed two couples of PCR-primers specific to amanitins and phallotoxins genes can be recommended for detection of Amanita phalloides and other mushroom species producing hepatotoxic cyclic peptides - amanitins and phallotoxins.
4
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

An algorithm for approximate identification of relaxation modulus of viscoelastic materials from non-ideal ramp-test histories

86%
Stankiewicz A.
Teka Komisji Motoryzacji i Energetyki Rolnictwa
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2013
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tom 13
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nr 1
5

Entrapment vectors - how to capture a functional transposable element

86%
Solyga A., Bartosik D.
Polish Journal of Microbiology
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2004
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tom 53
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nr 3
Transposable elements (i.e. insertion sequences and transposons) are components of nearly all bacterial genomes. The majority of these elements have been identified as a result of various sequencing projects. However, in most cases, their activity was not experimentally confirmed. For this reason several strategies have been developed that allow direct cloning and identification of functional transposable elements. Most of the methods are based on the ability of transposable elements to inactivate or activate particular genes by insertion. In this review we describe and critically discuss different cloning strategies that employ various entrapment vectors, carrying (i) conditionally lethal genes, (ii) antibiotic selection cartridges, (iii) promoter-less genes or (iv) suicide replicons. These tools, besides facilitating the identification of new transposable elements, also enable the investigation of various DNA rearrangement mutations, which are related to the transposition process.
6

Filamentous microorganisms occurring in foam of biological wastewater treatment plants and possibilities of their identification

86%
Kalisz L., Kazmierczuk M.
Archiwum Ochrony Środowiska
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2006
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tom 32
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nr 4
7

Results of indirect identification methods in polyploidy plants of Callistephus chinensis Nees

86%
Hanzelka P., Kobza F.
Roczniki Akademii Rolniczej w Poznaniu. Ogrodnictwo
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2000
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tom 31
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nr 1
8
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Multi-method approach to identification of species of Trochulus (Gastropoda: Hygromiidae)

72%
Prockow M., Strzala T., Kuznik-Kowalska E., Prockow J., Mackiewicz P.
Folia Malacologica
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2016
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tom 24
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nr 1
9

The methods used for the identification and differentiation of Campylobacter spp. in food products

72%
Mikolajczuk-Szczyrba A., Misiewicz A., Kieliszek M., Cendrowski A.
Zeszyty Problemowe Postępów Nauk Rolniczych
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2016
|
tom 585
Bacteria of Campylobacter genus remain most commonly reported gastrointestinal bacterial pathogen in humans in the EU since 2005. The main source of human infections caused by Campylobacter bacteria is the consumption of inadequately prepared, infected food products especially poultry meat, milk and dairy products, and contaminated water. High number of campylobacteriosis may us presume that in near future there will be implemented adequate changes in legislation regarding the microbiological criteria for food products, including the detection of Campylobacter spp. This paper contains literature review concerning identification and differentiation methods of Campylobacter genus in food. There are presented classical methods for Campylobacter spp. detection, based on biochemical and physiological properties and as well as molecular methods – PCR, multiplex PCR, PCR-RFLP, qPCR, dot blotting, FISH, which identifies characteristic DNA sequences.
10

Linking extracellular electric potential in the brain to neural activity - a review of source localization and component identification methods

72%
Leski S.
Acta Neurobiologiae Experimentalis
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2015
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tom 75
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nr 2
Due to technological advances in electrophysiology, there is renewed interest in the analysis of local field potentials recorded at many sites simultaneously. In this paper the main problems related to the analysis of LFP are presented, and recent developments in the data analysis methods are reviewed. The focus of the paper is on reconstruction of current source density from extracellular recordings and on decomposition of neural activity into meaningful components.
11

Sibling species of the Conocephalum conicum complex [Hepaticae, Conocephalaceae] in Poland: distribution maps and description of an identification method based on isoenzyme markers

72%
Odrzykoski I. J.
Fragmenta Floristica et Geobotanica
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1995
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tom 40
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nr 1
12

Morphological analysis of Sher River basin using GIS for identification of erosion-prone areas

58%
Deshmukh D. S., Chaube U. C., Tignath S., Tripathi S. K.
International Journal of Ecohydrology and Hydrobiology
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2010
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tom 10
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nr 2-4
The morphological analysis has been used to identify the erosion-prone sites in the Sher river basin which is one of the sub-basins of the greater Narmada basin, Madhya Pradesh, India. The Sher river basin has been divided into the forty five sub-basins of fourth order and fourteen sub-basin areas representing fifth, sixth and seventh order streams. The various morphological parameters have been derived for Sher river basin and its derived sub-basins to describe their topographical conditions, drainage patterns and their probable response to the runoff. The erosional risk prone map for the study area has been prepared using Texture-Slope Index and the erosional scale of the basin has been prepared using the same index. The derived erosional scale has been arranged into ‘0’ to ‘100’ numerical scale in which the base values ‘0’ and ‘100’ denote the least and the highest erosion prone areas respectively. Comparative study of the subbasins has been carried out by use of developed erosional scale. Classified erosional hazard map has been categorized into low, medium, high and very high sediment erosion. The classified map of erosional hazard may be useful to suggest various soil water conservation measures for water resources development and management.
13
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

SWOT analysis as a method allowing to identify the need for changes in a production process in wood industry

58%
Baskiewicz N.
Annals of Warsaw University of Life Sciences - SGGW. Forestry and Wood Technology
|
2013
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tom 82
14

Methods of finding and identification of pathogens in seeds

58%
Marcinkowska J. Z.
Plant Breeding and Seed Science
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2002
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tom 46
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nr 1
15
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Zastosowanie metod biologii molekularnej do identyfikacji grzybów zasiedlających martwe drewno sosny zwyczajnej (Pinus sylvestris L.)

51%
Behnke-Borowczyk J., Cichon J., Wolowska D., Haluszczak M., Baranowska-Wasilewska M.
Zarządzanie Ochroną Przyrody w Lasach
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2017
|
tom 11
16

A new method for species identification of poultry based on 12S-rRNA fragment polymorphism

44%
Natonek-Wisniewska M., Slota E.
Annals of Animal Science
|
2009
|
tom 09
|
nr 2
127-132
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