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Immuno-endocrine mechanisms connected with the creation of corpora lutea persistent in animal ovaries

100%

Kucharski J., Jana B.

Polish Journal of Veterinary Sciences

2005

tom 08

nr 3

Ghrelin role in hypothalamus-pituitary-ovarian axis

100%

Rak-Mardyla A.

Journal of Physiology and Pharmacology

2013

tom 64

nr 6

Biogenic amines and the activity of the hypothalamo-pituitary-ovarian axis in ewes

100%

Chomicka L. K.

Journal of Physiology and Pharmacology. Supplement

1992

tom 43

nr 1

Effect of leptin, insulin, and IGF-I on GnRH-induced LH secretion from porcine pituitary cells in vitro

100%

Kosior-Korzecka U., Bobowiec R.

Bulletin of the Veterinary Institute in Puławy

2007

tom 51

nr 3

The objective of this study was to analyse the response of porcine pituitary cells to leptin, insulin, and IGF-I in vitro. Pituitary cells were cultured in McCoy 5A medium without hormones (negative control), with GnRH (4x10⁻⁹M/L) (positive control), with GnRH and 10⁻¹¹-10⁻⁶ M/L of leptin, with GnRH and 3.9-91.0 mlU/L of insulin, or with GnRH and 1.03-39.2x10⁻⁹ M/L of IGF-I. After 6, 12, 18, 24, 30, and 36 h of cell incubation, the secretion of LH was determined. The obtained results showed that the effect of leptin and insulin on LH secretion from pituitary cells was dependent on the used dose of leptin. Leptin in concentrations 10⁻⁹, 10⁻⁸, and 10⁻⁷ M/L were significantly (P≤0.05) enhanced, whereas in concentration 10⁻⁶ M/L suppressed a LH secretion. Insulin at a dose from 3.9 to 13.0 mlU/L caused an increment, whereas in higher concentrations - a significant (P≤0.05) drop in LH secretion. Almost full positive correlation (r=0.98) between the level of IGF-I in the culture medium and LH secretion was found.

Ograniczanie wyników

1 Bulletin of the Veterinary Institute in Puławy

1 Journal of Physiology and Pharmacology

1 Journal of Physiology and Pharmacology. Supplement

1 Polish Journal of Veterinary Sciences

1 Bobowiec R.

1 Chomicka L. K.

1 Jana B.

1 Kosior-Korzecka U.

1 Kucharski J.

1 Rak-Mardyla A.

1 2013

1 2007

1 2005

1 1992

Wyniki wyszukiwania

Immuno-endocrine mechanisms connected with the creation of corpora lutea persistent in animal ovaries

Ghrelin role in hypothalamus-pituitary-ovarian axis

Biogenic amines and the activity of the hypothalamo-pituitary-ovarian axis in ewes

Effect of leptin, insulin, and IGF-I on GnRH-induced LH secretion from porcine pituitary cells in vitro