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The aim of this study was to analyse the association between factors characterising dairy herd management and the levels of somatic cell count (SCC), and total microorganisms count (TMC) in bulk tank milk. The investigations were carried out in 2005 in 187 family farms (herd size from 6 to 60 cows), situated in adjacent parts of Warmia & Mazury, and Mazowsze regions. Based on annual geometric means, all the farms were divided into those with low and elevated SCC level (<200,000 vs. 200,000–400,000 cells/mL), and with low and elevated TMC level in milk (<50,000 vs. 50,000–100,000 cfu/mL). The milk was characterised by low SCC level in 55 farms (29.4%), whereas TMC level was low in 65 farms (34.8%). The following factors were associated with the low level of SCC: small herd size, tie-stall system of cow housing, grazing cows, use of advisory service, use of antibiotics at cow dry-off, dry storing of milking clusters between milkings, post-milking teat dipping, regular use of California Mastitis Test, treatment of clinical cases of mastitis with antibiotics in selected cows only, use of whole straw in cow feeding, and use of salt-licks containing microminerals. Factors associated with the low level of TMC were: large herd size, colostrum feeding to the newborn calf by bucket, individual housing of replacement heifers older than 3 months, pipe-line system of milking, dry storing of milking clusters between milkings, pre-milking udder and teat cleaning with wet towel soaked with a disinfectant, tank system of milk cooling and storage after milking, and regular calculation of feeding rations for cows.
The objective of this study was to determine the influence of biological silage additive (Bonsilage) on the hygiene quality and nutritive value of maize and grass-legume silages. The experiments were conducted on FAO 240 maize (Zea mays L.) and a mixture of italian ryegrass (Lolium multiflorum L.), 50% with alfalfa (Medicago media Pers.), 50%. Group 1 was a control and comprised silage without any additives, group 2 was ensiled with the addition of 4 cm3 kg-1 biological silage additive. After 60 days of silage process individual silages were subjected to microbiological composition, and chemical analyses of silages were also determined. Similar analyses were repeated at day 7 following exposure to oxygen. The applied biological silage additive was found to reduce (P<0.05) numbers of Clostridium, Enterobacteriaceae, yeasts and mold fungi cells, and increase (P<0.05) the number of LAB (lactic acid bacteria) in comparison with the control in both silages. Chemical analysis of the maize silage showed that the biological additive caused an increase (P<0.05) in DM (dry matter), CP (crude protein), WSC (water soluble carbohydrates), LA (lactic acid), AA (acetic acid), ethanol, and a decrease (P<0.05) in the concentration of BA (butyric acid), N-NH3 and pH value in comparison with the control. Chemical analysis of silage samples from the grass-legume mixture showed that the additive caused an increase (P<0.05) in the content of DM, CP, WSC, LA and AA in comparison with the control. Samples of silage with the addition of an inoculant were characterized by a lower (P<0.05) content of BA, N-NH3, ethanol and pH value. The biological additive impoved the aerobic stability of silages in the aerobic phase.
The aim of this study was to determine the influence of sheep’s milk hygienic quality parameters on its composition and physical characteristics. During the three-year study 140 samples of Olkuska sheep milk were collected for analyzes. The samples were taken during the morning milking, and then were tested to determine the composition, physical properties, the presence of inhibitory substances, somatic cell count (SCC) and the total number of microorganisms. The increase in SCC and the total number of microorganisms caused an increase in dry matter content, total protein, casein, fat, total and soluble ash, pH, viscosity, conductivity and freezing point, and a decrease in lactose content, acidity and density. Statistically significant correlations were obtained for the relationship between SCC and the content of casein, fat, acidity, pH, density, and conductivity. As regards the total number of microorganisms most of the correlations were statistically significant, and the calculated correlation coefficients were higher than in case of SCC.
The hygienic quality and physicochemical properties of raw milk from Holstein-Friesian and Jersey cows were determined in the study. It was found that the total bacterial count and the somatic cell count in milk samples were within the threshold limit values specified by relevant legal regulations. Milk from Jersey cows contained much higher levels of dry matter, which resulted primarily from higher concentrations of protein compounds (by approx. 19%), both casein and whey proteins, as well as fat (by approx. 50%), as compared with milk from Holstein-Friesians. The levels of all analysed calcium forms (total, colloidal, soluble, ionic) were higher in milk from Jersey cows than in milk from Holstein-Friesian cows. The characteristics of the dispersion state of fat globules showed that their average diameters were greater (by approx. 24%) in milk from Jersey cows, in comparison with milk from Holstein-Friesians. The levels of lactose and milk properties, i.e. acidity, density, conductivity and freezing temperature, were similar in both cow breeds.
Fungal mastitis in cows is an increasing issue. The aim of the examination was to evaluate the species and enzymatic activity of yeast-like fungi from mastitis in cows. One hundred and twenty-six strains isolated from secretions of clinically and subclinically inflamed quarters of 102 cows were tested with the use of API 20 C AUX and then 63 strains were examined for enzymatic activity (API ZYM). Candida inconspicua (34.9% ), C. kefyr (19.9% ), C. krusei (11.1%) and S. cerevisiae (10.3%) were the mostly isolated species. Yeast from bovine mastitis showed activity of 12 enzymes, but alkaline phosphatase, valine arylamidase, acid phosphatase and Naphthol-AS-B1-phosphohydrolase were stated at the highest level.
The aim of this study was to evaluate milk hygienic quality and to determine a relationship between season of year and raw milk quality indices. The experiment was carried out on 38 samples of milk collected from 80 high–production cows managed in a leading cattle breeding centre in south–west Poland. The analysis comprised total bacteria count, somatic cell count in 1 cm3 of milk, chemical composition assay, i.e. fat and protein content, freezing point determination and test for inhibitory substances. The quality of milk was evaluated instrumentally, and the analysis for inhibitory substances was done using Delwotest assay. Both chemical composition and hygienic quality were within the standard set for the Extra class of raw milk. The season of year significantly influenced the chemical composition of milk and somatic cell count, however, it has no effect on freezing point or total bacteria count.
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Respiratory exposure to organic dust induces chronic pulmonary diseases both in farmers and horses. The aim of this study was to examine the variation of dustiness and hygiene quality of peat moss bedding. Materials studied were weakly decomposed sphagnum peat (A), weakly decomposed sphagnum peat warmed up in storage (>30oC) (B) and two more decomposed few-flowered sedge peats (C and D). The geometric mean of mesophilic fungi, thermotolerant fungi and thermophilic actinomycetes were determined from the material. Samples of inhalable dust and endotoxins were collected with IOM samplers and respirable dust with 10M foam samplers when the peat was rotated in a cylinder. The number of particles was detected with an optical particle counter. An LAL assay was used for analysing endotoxins from the filter samples. There were differences in the hygiene quality and dustiness between peat materials (p < 0.01). The geometric mean of fungi was smallest in material A. Warming-up increased the number of fungi in sphagnum peat, but on the other hand, it decreased the content of endotoxin (p < 0.01). Few-flowered peat materials contained thermophilic actinomycetes and material D also contained Aspergillus fumigatus. The concentrations of inhalable dust, respirable dust and the number of particles were smaller in the few-flowered peats (C-D) than in the sphagnum peats (A-B). It is concluded that there are differences in the dustiness and hygiene quality of peat bedding.
The investigation of relationships between collection system, season and monthly delivery size and somatic cell count in 1 ml of milk classified to the highest quality classes (Extra and the First) in one of leading dairy co-operatives from the region of Central Poland was the aim of the study. Somatic cell count (SCC) of individual milk supplies constituted the research material. Results confirmed that milk collected directly from the farm by autocysternes had much lower SCC level. The increase of the average monthly milk supply had positive relationship with its hygienic quality. The worst milk quality was noticed in the summer season, i.e. in June, July and August, regardless of delivery system. The results show further possibilities of improvement of hygienic conditions of milk production and collection in Poland.
Ocenie poddano jakość higieniczną twarogów produkowanych w różnych zakładach mleczarskich. W produktach oznaczano zmiany liczebności populacji enterokoków i gronkowców. Wyniki badań wykazały, że jakość higieniczna twarogów po 14 dniach przechowywania była zależna nie tylko od rodzaju zakładu mleczarskiego, w którym wytwarzano produkty ale od poziomu początkowego obu populacji bakterii i od rodzaju stosowanego opakowania. Bez względu па jakość zakladu produkującego twarogi między 3 а 6 dniem przechowywania populacja gronkowców kształtowała się na poziomie 1,5 do 2,5 log jtk/g. Stwierdzono istotną rolę interakcji między gronkowcami i enterokokami w kształtowaniu jakości higienicznej twarogów przechowywanych przez okres 14 dni.
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