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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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The activity of hydrolases of larval stages of Anisakis simplex [Nematoda]

100%
Lopienska-Biernat E., Zoltowska K., Rokicki J.
Wiadomości Parazytologiczne
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2004
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tom 50
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nr 3
2
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Geotrichum candidum Link 1809: hydrolases activity and own methoof digestive tract strains biotyping

100%
Kurnatowska A., Bialasiewicz D.
Wiadomości Parazytologiczne
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2001
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tom 47
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nr 4
Hydrolase activity of (enzymograms, biotypes) in Geotrichum candidum, one of the poorly described pathogenic fungi, was studied 81 strains were isolated from oral cavity and faeces of patients with gastrointestinal tract disorders. Axenic strains were differentiated with API 20C Aux and API ZYM tests. Then, enzymograms and biotypes were determined for all strains based on the activity of 19 hydrolases. High variability of enzymograms (17 different types) was found. The highest activity was noted in case of: e2-alkaline phosphatase, e6-lecucine arylamidase, e11-acid phosphatase. E5-lipase. e7-valine arylamidase, e12 - naphtol-AS-BI-phosphohydrolase and e17-β-glucosidase were used for biotyping procedures. Our own system of biotyping of 81 strains of G. candidum was based on the mathematical binominal distribution formula (1 : 4 : 6 : 4: 1) – all; ,,+”;one „-”, three „+”; two „-”,two „+”; three ,,-”,one ,,+”; all „-”. We have found: A (11.1 ± 3.5%), B1 (6.17 ± 2.67%). B2 (1.23 ± 1.22%), B4 (4.94 ± 2.41%), C1 (1.23 ±1.22%), C3 (63.0 ± 5.4%), D2 (9.88 ±3.31%), D3 (2.47 ± 1.72%). Among all strains from 8 various biotypes of G. candidum.
3
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Candida albicans Berthout, 1923: Hydrolases activity and own method of digestive tract strains biotyping

100%
Kurnatowski M., Wasowska-Krolikowska K., Kurnatowska A.
Wiadomości Parazytologiczne
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2002
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tom 48
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nr 4
112 strains of Candida albicans were isolated from oral cavity and ontocenoses of the upper digestive tract (endoscopy) of children (age: 5-17) with gastrointestinal disorders. Axenic strains were differentiated with API 20C AUX and API ZYM tests (bioMerieux). Then enzymograms and biotypes were determined for all the strains based on the activity of 19 hydrolases. The highest activity was noted for: e₂ - phosphatase alcaline, e₆ – leucine arylamidase, e₁₁ - phosphatase acid, e₅ - lipase (c₁₄); e₇ - valine arylamidase, e₁₂ - naphtol-AS-BI-phosphohydrolase, e₁₆ - α-glucosidase and e₁₈ - N-acetyl-ß-glucosamidase, and the latter four were used for biotyping procedures. Our own system was based on the mathematical binominal distribution formula (1: 4: 6: 4: 1): all „+”; one „ -” three „+”; two „-”, two ,,+”; tbree „-”, one „+”; all „-”. We bave found the following biotypes: A (16.1 ± 3.5%), B₁ (2.7 ± 2.53%), B₃ (8.0 ± 25%), B₄ (22.3 ± 3.9%), C₂ (1.8 ± 1.3%), C₃ (7.1 ± 2.4%), C₆ (30.4 ± 4.3%), D₃ (11.6 ± 3.0%).
4
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The activity of hydrolases of entomopathogenic nematodes

86%
Zoltowska K., Lopienska E.
Wiadomości Parazytologiczne
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2003
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tom 49
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nr 4
Similar patterns of hydrolases were observed in three species representing two genera of entomopathogenic nematodes: Steinernema affinis, S. feltiae, and Heterorhabditis zealandica. The same enzymes were present in the studied nematodes but they differed in the level of activity of individual sub-classes of enzymes. A higher activity of esterases and proteolytic enzymes could be observed for H. zealandica than for S. affinis and S. feltiae. On the other hand, the activity of glycosidases in steinernematids was generally much higher than in H. Zealandica.
5
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Activity of hydrolases in the reproductive system and in consecutive stages of Ascaris suum development

72%
Dziekonska-Rynko J.
Wiadomości Parazytologiczne
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2004
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tom 50
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nr 2
6

Geotrichum hydrolytic activity in milled malt and barley medium

58%
Piegza M., Witkowska D., Stempniewicz R., Rywinska A.
Electronic Journal of Polish Agricultural Universities. Series: Biotechnology
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2005
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tom 08
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nr 1
7
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Effects of farmyard manure and nitrogen fertilizers on mobility of phosphorus and sulphur in wheat and activity of selected hydrolases in soil

58%
Lemanowicz J., Siwik-Ziomek A., Koper J.
International Agrophysics
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2014
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tom 28
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nr 1
The paper demonstrates the results of research on the mobility of phosphorus and sulphur in winter wheat fertilized with several rates (0, 20, 40, 60, 80 t ha-1) of farmyard manure and nitrogen (0, 40, 80, 120 kg N ha-1). The content of these nutrients was related to the activity of acid phosphatase and arylsulphatase in a Haplic Luvisol. The highest content of available phosphorus (91.58 mg P kg-1) was reported in the soil amended with farmyard manure at the rate of 60 t ha-1. The content of sulphates (VI) in the Haplic Luvisol was high and, on average, equal to 25.22 mg kg-1. The activity of acid phosphatase in the soil increased with increasing mineral nitrogen rates. The highest content of sulphates (VI) and the lowest activity of arylsulphatase were identified at the nitrogen rate of 40 kg N ha-1. The mobility of phosphorus in winter wheat was the highest when farmyard manure at the rate of 60 t ha-1 and mineral nitrogen at the rate of 120 kg N ha-1 were incorporated into the soil. The greatest translocation of sulphur was reported at the high farmyard manure rates (40, 60 and 80 t ha-1) and the nitrogen rate of 80 kg N ha-1.
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