Ograniczanie wyników

Czasopisma help
Autorzy help
Lata help
Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 218

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 11 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych:  hybridization
help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 11 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
Fluorescence in situ hybridization (FISH) experiments with specific probes for chromosome 29 and 25 were carried out on a Brown Swiss bull, previously diagnosed as a carrier of 1;29 centric fusion. The hybridization of the chromosome 29-specific probe (BMC 4216 - already located on 29q13), produced signals on two small acrocentrics, but not on the translocated chromosome. The signals appeared on the translocated chromosome and on a single chromosome 25 after hybridization of the chromosome-specific probe (BMC 3224 - previously located on 25q24). According to the actual nomenclature, the analysed aberration is a robertsonian translocation involving chromosomes 1 and 25.
Fluorescence in situ hybridization (FISH) is a very useful method for assessing chromosome rearrangements. When neither banding pattern nor clinical symptoms are sufficient to determine the origin of additional chromosomal fragment, FISH with multiple chromosome-specific libraries (chromosome painting), allows to solve this diagnostic problem rapidly. Three chromosomal additions, 7q+, 13p+ and 22q+, found in routine cytogenetic studies performed in children with phenotypic abnormalities were analysed using FISH. This technique documented the origin of the extra material to be derived from chromosome 16[der(7)t(7; 16)(q36.3;p 13.11)], 18[der(13)t(13; 18)(p12;q 12.2)] and 22[dup(22)(q11.2q13.1)], respectively. In two cases the abnormality arose de novo, while in the third case the product of translocation t(13;18) was maternal by origin. It was present in 30% of mother's lymphocytes, and in 70% of them a balanced Robertsonian translocation t(13q;15q) was found. In the presented cases the chromosome analysis with both traditional banding and chromosome painting techniques, allowed to establish final clinical diagnosis.
In Southern blot analysis of Paramecium genomic DNA using β-adrenergic specific oligonucleotides labelled with Digoxigenin-ddUTP, the DNA species of the same molecular size were detected by different molecular probes. The 6.5 kb DNA species hybridized with the probes directed to the adjacent β2-receptor transmembrane domains: to TM3 - including Asp 113 involved in β-agonists and antagonists binding, and to TM4 - the oligonucleotide being one of the "gene-specific universal mammalian sequence-tagged site”. Since a profound physiological effect of beta-agonists and antagonists has been previously observed in Paramecium, such a hybridization pattern may suggest an existence of Paramecium DNA sequences homologous to those of metazoan eukaryotes encoding for a membrane beta-adrenergic receptor.
Natural hybridization occurs commonly in plants and blurs their interspecific delimitation. It is unclear whether spontaneous hybridization occurs in natural populations of Primula in China. In this study we examined natural hybridization between P. secundiflora and P. poissonii based on morphological and molecular analyses of individuals with intermediate morphology. Most morphological characters of these individuals were found to be intermediate between the putative parental species, but plant height and corolla tube length were significantly transgressive. Molecular analyses based on nuclear internal transcribed spacer (ITS) additivity and two types of cloned sequences (each corresponding respectively to one of two parental species) clearly suggested that these individuals result from hybridization between P. secundiflora and P. poissonii. We further used a maternally inherited chloroplast DNA fragment (rpl16 intron) to trace the maternal composition of the hybrids. Among 26 hybrids, ten (two thrums, eight pins) had the P. secundiflora haplotype, while 16 (ten thrums, six pins) possessed the P. poissonii haplotype. These results suggest that both parents served as the mother donors of the hybrids. Reciprocal hybridization between the two species seems to be symmetrical rather than unidirectional
Abies fraseri (FF) is a North American conifer which is crucial for growers of Christmas trees. This species suffers 100% mortality after infection of root rot caused by Phytophthora cinnamomi. The hybrid material A. cilicica × A. cephalonica (CZ) and A. koreana × (A. cilicica × A. cephalonica) was utilized as mother trees for applications of Abies fraseri pollen. In addition to resistance to fungal disease, a long-term monitoring of growth of young seedlings along with the impact of artificial crossing on morphology of the progeny is covered. Except for increased resistance, some offspring has also exhibited a remarkable heterosis effect at younger age and different growth habit and different shape of their needles. All this may offer in the future a wide range of applications, both for crops under strong anthropogenic pressure with changing climatic conditions and for crops grown for production of Christmas trees and outstanding ornamental cultivars. Annual crossing success was determined in present study using X-ray imaging. The most successful combinations in 2012 were those of CZ1 × FF25 and CZ1 × FF76 with 7% of full seeds, followed by CZ2 × FF25 with 6% and CZ2 × FF31 with 4% of the full seeds. The evaluation of young seedlings from control crossing in 2007 highlighted significant accented height growth of complicated hybrid (A. koreana × (A. cilicica × A. cephalonica)) × FF PC. Morphology of needles showed variability in their length as compared with the needle width which was found to be less appropriate characteristic for distinguishing between different combinations.
Laboratory hybridization studies were performed on the springtail species Bonetogastrura cavicola (Börner, 1901) and Ceratophysella impedita (Skarżyński, 2002). The studies revealed that the species under consideration can not produce viable offspring, but they can produce numerous eggs and some hybrid embryo. Consequently both species were recognized as closely related and members of the genus Ceratophysella Börner, 1932.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 11 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.