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The activity of human telomerase in the cells of acute leukaemias

100%

Kocki J., Kolano J., Cioch M., Dmoszynska A., Wojcierowski J.

Folia Morphologica

2004

tom 63

nr 1

Telomeres are the end fragments of chromosomes formed by a number of non-coding double-stranded TTAGGG repeats in vertebrates. During cell division the number of repeats decreases, leading to cell senescence or apoptosis. In immortal cells, including cancer cells, the telomere length is stable and maintained by, among other factors, telomerase. The aim of the study is to compare telomerase activity in normal lymphocytes and in leukaemic cells. Samples of acute leukaemia cells, HL 60 cell line and the lymphocytes of healthy volunteers were examined. Telomerase analysis was performed using TeloTAGGG Telomerase PCR ELISAplus (Roche). The relative telomerase activities (RTA) in leukaemic and normal cells were analysed. A high level of RTA was observed in leukaemic cells.

The human telomerase catalytic subunit and viral telomerase RNA reconstitute a functional telomerase complex in a cell-free system, but not in human cells

80%

Trapp-Fragnet L., Marie-Egyptienne D. T., Fakhoury J., Rasschaert D., Autexier C.

Cellular and Molecular Biology Letters

2012

tom 17

nr 4

The minimal vertebrate telomerase enzyme is composed of a protein component (telomerase reverse transcriptase, TERT) and an RNA component (telomerase RNA, TR). Expression of these two subunits is sufficient to reconstitute telomerase activity in vitro, while the formation of a holoenzyme comprising telomerase-associated proteins is necessary for proper telomere length maintenance. Previous reports demonstrated the high processivity of the human telomerase complex and the interspecies compatibility of human TERT (hTERT). In this study, we tested the function of the only known viral telomerase RNA subunit (vTR) in association with human telomerase, both in a cell-free system and in human cells. When vTR is assembled with hTERT in a cell-free environment, it is able to interact with hTERT and to reconstitute telomerase activity. However, in human cells, vTR does not reconstitute telomerase activity and could not be detected in the human telomerase complex, suggesting that vTR is not able to interact properly with the proteins constituting the human telomerase holoenzyme.

Ograniczanie wyników

1 Cellular and Molecular Biology Letters

1 Folia Morphologica

1 Autexier C.

1 Cioch M.

1 Dmoszynska A.

1 Fakhoury J.

1 Kocki J.

1 Kolano J.

1 Marie-Egyptienne D. T.

1 Rasschaert D.

1 Trapp-Fragnet L.

1 Wojcierowski J.

1 2012

1 2004

Wyniki wyszukiwania

The activity of human telomerase in the cells of acute leukaemias

The human telomerase catalytic subunit and viral telomerase RNA reconstitute a functional telomerase complex in a cell-free system, but not in human cells