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Diagnostic values of calcium and magnesium forms determined in human serum and saliva

100%

Andrusishina I. N.

Journal of Elementology

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2010

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tom 15

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nr 3

425-433

Calcium and magnesium are known to be necessary for the normal function of various systems in animal and human organisms. There are many diseases caused by abnormal concentration of electrolytes, e.g. arterial hypertension or nervous system diseases such as multiple sclerosis, Mb. Alzheimer or Mb. Parkinson. The mechanisms of homeostasis indicate only the ionized forms of these elements. It is known that ionized calcium serves as an endocellular intermediary in action of enzymes and hormones in cells. Therefore, it is very important to define levels of total and ionized forms of Ca2+ and Mg2+ in blood serum and saliva by the method of atomic absorption spectrometry and to show their diagnostic value for various pathological conditions of a human body. The 39 persons, aged 21 to 47 years take part in these investigations. The results of determinations of calcium and magnesium forms present in human serum and saliva, representing physiological states are presented. The age and daily fluctuations of Ca2+ and Mg2+ content in serum and saliva were studied by atomic absorption spectrometry. The levels of non albumin forms of these elements were found by FAAS. The significance of determination of calcium and magnesium levels in serum and saliva under various pathological conditions (arterial hypertension and osteoporosis) was shown.

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Determination of adenine nucleotides and their metabolites in human saliva

100%

Kochanska B., Smolenski R. T., Knap N.

Acta Biochimica Polonica

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2000

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tom 47

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nr 3

The profile and normal concentrations of nucleotide metabolites in human saliva and reproducibility of these determinations were analyzed. Samples of human saliva collected from healthy individuals at weekly intervals, were deproteinized and analysed for the content of adenine nucleotides and their metabolites by reversed-phase HPLC. Initial ATP, hypoxanthine and uric acid concentrations were 0.52 0.15 µM, 1.91 0.37 µM and 184 22 µM respectively. A substantial individual variation persisted within 3 weeks of sampling excepted hypoxanthine which showed some unrelated variations. Determination of nucleotides and their catabolites in saliva due to its simplicity and reproducibility, may be of clinical value in diagnosis of local or systemic disorders.

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Artificial saliva and its use in biological experiments

80%

Pytko-Polonczyk J., Jakubik A., Przeklasa-Bierowiec A., Muszynska B.

Journal of Physiology and Pharmacology

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2017

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tom 68

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nr 6

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Journal of Elementology

1 Journal of Physiology and Pharmacology

1 Andrusishina I. N.

1 Jakubik A.

1 Knap N.

1 Kochanska B.

1 Muszynska B.

1 Przeklasa-Bierowiec A.

1 Pytko-Polonczyk J.

1 Smolenski R. T.

1 2017

1 2010

1 2000

Diagnostic values of calcium and magnesium forms determined in human serum and saliva

Determination of adenine nucleotides and their metabolites in human saliva

Artificial saliva and its use in biological experiments