Wyniki wyszukiwania

1

One-step purification of vitronectin from human plasma by affinity chromatography on phage-displayed peptides

100%

Skurzynski S.

Acta Biochimica Polonica

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2010

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tom 57

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nr 1

89-93

A novel affinity purification method for rapid isolation of vitronectin (VN) from human plasma is described. Recently we have used phage display technology to obtain clones expressing peptides with high binding activity for VN. The isolated "strong VN binders" were covalently coupled to CNBr-activated Sepharose. Human plasma was applied to the column and bound VN was eluted using 0.5 M acetic acid, giving purity exceeding 90 %. The developed method is a convenient alternative to conventional antibody-antigen affinity chromatography techniques for purification of VN, as it offers low ligand cost, is rapid and ensures good protein recovery from human plasma.

2

The reactivity of cellular proteins extracted from calcified human valves with human plasma

86%

Stepien E., Pfitzner R., Przybylo M., JuchnoA., Litynska A., Sadowski J.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

3

Human plasma and cerebrospinal fibronectins differ in the accessibility of the epitopes on the N-terminal domains

72%

Pupek M., Lemanska-Perek A., Jasonek J., Katnik-Prastowska I.

Acta Biochimica Polonica

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2010

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tom 57

|

nr 3

Three monoclonal antibodies specific to the central cell-binding and the C- and N-terminal domains of fibronectin (FN) were used to test antigenic epitope accessibility on human plasma and cerebrospinal fibronectins. In the plasma group, the mean N-terminal FN domain immunoreactivity was about one fourth that of the cell-binding and C-terminal domains, whereas in cerebrospinal fluid they were nearly equal. In the presence of 0.5-6 M urea N-terminal domain immunoreactivity in the plasma increased 3-6-fold, but it decreased 0.7-3-fold in the cerebrospinal fluid. Analysis of fibronectin domain immunoreactivities of the cell-binding and N-terminal domains by a panel of specific monoclonal antibodies may reveal N-terminal fibronectin domain accessibility for reaction with biological partner ligand(s) and/or processes in which FN could be implicated. Such determinations may have important clinical implications.

4

Antisense hairpin loop oligonucleotides as inhibitors of expression of multidrug resistance-associated protein 1: Their stability in fetal calf serum and human plasma

72%

Rebowski G., Wojcik M., Boczkowska M., Gendaszewska E., Soszynski M., Bartosz G., Niewiarowski W.

Acta Biochimica Polonica

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2001

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tom 48

|

nr 4

Multidrug resistance-associated protein (MRP1) is a transmembrane pump protein responsible for the efflux of chemotherapeutic drugs, an important cause of anticancer treatment failure. Trying to circumvent MRP-mediated resistance we designed and synthesized hairpin loops forming antisense oligodeoxyribonucleotides (ODNs), both phosphodiesters (PO-ODNs) and their phosphorothioate analogues (PS-ODNs), to reduce the protein expression by targeting its mRNA in a sequence specific manner. Melting temperature measurements as well as polyacrylamide gel elec- trophoresis supported the preferential formation of a secondary structure, which was expected to protect ODNs against 3-exonuclease degradation. ODNs and PS-ODNs designed in this work were successfully tested as antisense inhibitors of the expression of MRP1 in the leukaemia HL60/ADR cell line. Foreseeing the necessity to perform clinical studies with such ODNs we investigated their stability against the 3 -exonuclease activity of fetal calf serum and human plasma. Under the conditions, corresponding to physiological ones, we observed high stability of hairpin loop forming ODNs, especially those containing longer (e.g. 7 base pair) stems. Comparative studies on the stability of chemically unmodified hairpin loop forming ODNs and their PS-counterparts indicated that endonuclease activity did not play any important role in the process of their nucleolytic degradation. Our studies provide strong evidence for high stability of chemically unmodified hairpin loop ODNs, making them an attractive alternative to phosphorothioate analogues commonly used in antisense strategy.

5

The effect of the Glu342Lys mutation in alpha1-antitrypsin on its structure, studied by molecular modelling medhods

72%

Jezierski G., Pasenkiewicz-Gierula M.

Acta Biochimica Polonica

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2001

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tom 48

|

nr 1

The structure of native α1-antitrypsin, the most abundant protease inhibitor in human plasma, is characterised primarily by a reactive loop containing the centre of proteinase inhibition, and aβ-sheet composed of five strands. Mobility of the reactive loop is confined as a result of electrostatic interactions between side chains of Glu342 and Lys290, both located at the junction of the reactive loop and the β structure. The most common mutation in the protein, resulting in its inactivation, is Glu342->Lys, named the Z mutation. The main goal of this work was to investigate the influence of the Z mutation on the structure of α1-antitrypsin. Commonly used molecular modelling methods have been applied in a comparative study of two protein models: the wild type and the Z mutant. The results indicate that the Z mutation introduces local instabilities in the region of the reactive loop. Moreover, even parts of the protein located far apart from the mutation region are affected. The Z mutation causes a relative change in the total energy of about 3%. Relatively small root mean square differences between the optimised structures of the wild type and the Z mutant, together with detailed analysis of 'conformational searching' process, lead to the hypothesis that the Z mutation principally induces a change in the dynamics of α1-antitrypsin.

6

The use immobilized methylchymotrypsin for the purification of human and sheep alpha-1-proteinase inhibitor [alpha1-PI]

72%

Grybel J., Wilusz T.

Cellular and Molecular Biology Letters

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2003

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tom 08

|

nr 2

α1-proteinase inhibitor was isolated from albumin fractions of human and sheep plasma using a new method of purification using affinity chromatography on immobilized methylchymotrypsin in the presence of 5 M NaCl. The inhibitor was finally polished to homogenity either by chromatography on a Mono Q or a Sephacryl S-200 HR column. The presented method makes it possible to recover α1-proteinase inhibitor which has been added to cow milk.

7

The protective effects of selenoorganic compounds against peroxynitrite-induced changes in plasma proteins and lipids

58%

Nowak P., Saluk-Juszczak J., Olas B., Kolodziejczyk J., Wachowicz B.

Cellular and Molecular Biology Letters

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2006

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tom 11

|

nr 1

Many selenoorganic compounds play an important role in biochemical processes and act as antioxidants, enzyme inhibitors or drugs. The effects of a new selenocompound — bis(2-aminophenyl)-diselenide on oxidative/nitrative changes in human plasma proteins induced by peroxynitrite (ONOO−) were studied in vitro and compared with the those of ebselen, a well-known antioxidant. We also studied the role of the tested selenocompounds in peroxynitrite-induced plasma lipid peroxidation. Exposure of the plasma to peroxynitrite (0.1 mM) resulted in an increase in the level of carbonyl groups and nitrotyrosine residues in plasma proteins (estimated using the ELISA method and Western blot analysis). In the presence of different concentrations (0.025–0.1 mM) of the tested selenocompounds, 0.1 mM peroxynitrite caused a distinct decrease in the level of carbonyl group formation and tyrosine nitration in plasma proteins. Moreover, these selenocompounds also inhibited plasma lipid peroxidation induced by ONOO−1 (0.1 mM). The obtained results indicate that in vitro bis(2-aminophenyl)-diselenide and ebselen have very similar protective effects against peroxynitrite-induced oxidative/nitrative damage to human plasma proteins and lipids.

8

The preparation of specific sorbents with polyclonal antibodies as a ligand for purification of human antithrombin III

58%

Rogalski J., Dawidowicz A. L., Wiater A., Gibula K., Winiarczyk S.

Acta Microbiologica Polonica

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1998

|

tom 47

|

nr 2

9

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The effect of NO-EDRF and monocytes-macrophages on LDL-oxidation

51%

Leitinger N., Oguogho A., Rodrigues M., Sinzinger H.

Journal of Physiology and Pharmacology

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1995

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tom 46

|

nr 4

10

A new alpha-1-antitrypsin phenotype [PiZ Lodz] in Polish population with reduced level in serum

44%

Pilacik B., Kowalska A., Rujner J., Deron Z.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1992

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tom 40

|

nr 3

11

The occurrence of siderophores in Staphylococci

44%

Lisiecki P., Tkacz B., Sobis M., Mikucki J.

Acta Microbiologica Polonica

|

1994

|

tom 43

|

nr 1

Ograniczanie wyników

One-step purification of vitronectin from human plasma by affinity chromatography on phage-displayed peptides

The reactivity of cellular proteins extracted from calcified human valves with human plasma

Human plasma and cerebrospinal fibronectins differ in the accessibility of the epitopes on the N-terminal domains

Antisense hairpin loop oligonucleotides as inhibitors of expression of multidrug resistance-associated protein 1: Their stability in fetal calf serum and human plasma

The effect of the Glu342Lys mutation in alpha1-antitrypsin on its structure, studied by molecular modelling medhods

The use immobilized methylchymotrypsin for the purification of human and sheep alpha-1-proteinase inhibitor [alpha1-PI]

The protective effects of selenoorganic compounds against peroxynitrite-induced changes in plasma proteins and lipids

The preparation of specific sorbents with polyclonal antibodies as a ligand for purification of human antithrombin III

The effect of NO-EDRF and monocytes-macrophages on LDL-oxidation

A new alpha-1-antitrypsin phenotype [PiZ Lodz] in Polish population with reduced level in serum

The occurrence of siderophores in Staphylococci