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1

Detection of CMV infected cells by flow cytometry - evaluation of MAbs CCH2 and AAC10 directed against early and late CMV antigens

100%
Siennicka J.
Polish Journal of Microbiology
|
2004
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tom 53
|
nr 2
In this work evaluation of usefulness of monoclonal antibodies (MAbs) CCH2 and AAC10 directed against early - pUL44(DB52) and late - ppUL83(pp65) CMV antigens, utilized in Department of Virology, NIH for routine diagnosis of CMV infection by shell vial and pp65 antigenemia assay, for determination of CMV antigens by flow cytometry in human leucocytes, isolated, infected and cultivated in vitro was presented.
2

HCMV infection - problems of laboratory diagnosis

86%
Siennicka J., Zuk A., Rechnio M., Litwinska B.
Bulletin of the Veterinary Institute in Puławy. Supplement
|
2002
3

Characteristics of MNNG induced repair synthesis and DNA synthesis induced by human cytomegalovirus

72%
Anuszewska E., Koziorowska J.
Acta Biochimica Polonica
|
1989
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tom 36
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nr 2
4

Application of PCR in situ method for detection of human cytomegalovirus [HCMV] DNA

72%
Kosz-Vnenchak M., Grabiec A., Zawilinska B.
Acta Microbiologica Polonica
|
2001
|
tom 50
|
nr 2
5

Molecular analysis of the human cytomegalovirus strains isolated from infected infants

72%
Augustynowicz E., Dzierzanowska D.
Acta Microbiologica Polonica
|
1998
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tom 47
|
nr 4
6

Cytomegalovirus immediate early gene UL37 encodes a novel MHC-like protein

72%
Wyrwicz L. S., Rychlewski L.
Acta Biochimica Polonica
|
2008
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tom 55
|
nr 1
The cytomegalovirus (CMV) genome encodes four clusters of genes expressed immediately after infection — i.e.: UL36-38, UL122-123, TRS1-IRS1, and US3. The general function of these genes is associated with inhibition of cellular mechanisms of antiviral response. Although several biological processes have been mapped onto specific gene products, the knowledge of the molecular mechanism of their activity remains fragmentary. Here, we report the application of protein structure prediction methods in assigning the function to a glycosylated domain encoded by UL37 of CMV (gpUL37, UL37x3). The discerned similarity clearly points out that this domain represents a novel type of a major histocompatibility complex (MHC)-like protein, and consequently may play a central role in an additional mechanism of escape from antiviral response.
7

Cellular expression of TNF-alpha in human cytomegalovirus [HCMV] infection

72%
Kasprzak A., Zabel M., Surdyk-Zasada J., Seidel J., Filipiak B.
Folia Histochemica et Cytobiologica
|
2001
|
tom 39
|
nr 2
8
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Human cytomegalovirus infection as a lifelong health problem

58%
Figlerowicz M., Modlinska-Cwalinska A., Mania A., Mazur-Melewska K., Kemnitz P., Jonczyk-Potoczna K., Sluzewski W.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2011
|
tom 92
|
nr 4
9

Detection of cytomegalovirus in infant cerebrospinal fluid by conventional PCR, nested PCR and PCR-Digene

58%
Kapusta M., Dzierzanowska D., Dunin-Wasowicz D., Milewska-Bobula B., Dobrzanska A., Wojda U., Swiatkowska E., Blaszczyk G.
Acta Microbiologica Polonica
|
2001
|
tom 50
|
nr 3-4
263-274
10

The construction of the eukaryotic expression plasmid pcDNA3.1-azurin and the increased apoptosis of U2OS cells transfected with it

58%
Ye Z., Peng H., Fang Y., Feng J., Yang D. S.
Cellular and Molecular Biology Letters
|
2007
|
tom 12
|
nr 3
In our previous study, we demonstrated that azurin could selectively trigger apoptosis in human osteosarcoma cell line U2OS cells. However, the rate of apoptosis (35.8 ± 3.2%) is not very high, and azurin is too expensive to obtain readily. To solve these problems, we constructed a eukaryotic expression plasmid containing the azurin gene with an influenza virus haemagglutinin 9 peptide HA epitope tag, and transfected the recombinant plasmid pcDNA3.1(+)/azurin into U2OS cells. RT-PCR and Western blot analysis validated the successful transfection and the expression of the azurin-HA protein. Conspicuous apoptosis of the transfected cells was detected by flow cytometry (FCM) and the DNA ladder test. The apoptosis rate reached 64.3 ± 13.1%. The transcriptional levels of the Bax and p53 genes increased significantly in U2OS cells transfected with pcDNA3.1(+)/azurin, but the Bcl-2 mRNA level decreased. There was no difference in the levels of Bcl-xl mRNA and Survivin mRNA. We propose that the transfection of the recombinant plasmid pcDNA3.1(+)/azurin can significantly induce apoptosis in U2OS cells. This is closely associated with the up-regulation of the transcriptional level of the Bax and p53 genes, and the down-regulation of that of the Bcl-2 gene.
11

Protection of cattle against bovine leukemia virus [BLV] infection could be attained by DNA vaccination

58%
Brillowska A., Dabrowski S., Rulka J., Kubis P., Buzala E., Kur J.
Acta Biochimica Polonica
|
1999
|
tom 46
|
nr 4
The bovine leukemia virus (BLV) envelope gene encoding extracellular glycoprotein gp51 and transmembrane glycoprotein gp30 was cloned into a vehicle expression vector under the human cytomegalovirus (CMV) intermediate early promoter. The intramuscular injection of this plasmid vector generated a cellular immune response. Seven out of ten cows vaccinated with the DNA construct resisted a drastic challenge (500 BLV-infected lymphocytes as an infectious dose).
12

Expression of mRNA for cytokines [TNF-alpha and IL-1alpha] in human cytomegalovirus [HCMV] and hepatitis B virus [HBV] infections

58%
Kasprzak A., Zabel M., Wysocki J., Seidel J., Surdyk-Zasada J., Filipiak B.
Folia Histochemica et Cytobiologica
|
2002
|
tom 40
|
nr 2
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