Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: human anaplasmosis

Sortuj według:

Ogranicz wyniki do:

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Human anaplasmosis in North-Eastern Poland: seroprevalence in humans and prevalence in Ixodes ricinus ticks

100%

Grzeszczuk A., Stanczak J., Kubica-Biernat B., Racewicz M., Kruminis-Lozowska W., Prokopowicz D.

Annals of Agricultural and Environmental Medicine

2004

tom 11

nr 1

Sera of 500 inhabitants of north-eastern Poland, 450 suspected for Lyme borreliosis and 50 blood donors (control group), were analysed for the presence of IgG antibodies against Anaplasma phagocytophilum, the causative agent of human anaplasmosis (HA), known so far as human granulocytic ehrlichiosis (HGE). Forty one (9.1%) sera of the study group and one serum (2%) of the control group were positive using indirect fluorescence assay (IFA). The seropositivity tended to be more frequent among males (10.3%) than females (7.6%) and among the rural (10.3%) than urban population (7.5%); however, differences were of no statistical significance (p = 0.4). No age difference was found between the seropositive and the seronegative individuals (p = 0.77). The only factor increasing the risk of HA seropositivity found was forestry employment (p < 0.05). Additionally, a total of 559 Ixodes ricinus ticks, collected in the same area as sera, were investigated for the presence of A. phagocytophilum by the polymerase chain reaction (PCR) and 41 (8.7%) of them were found to be positive. The infection level ranged from 2.3-13.7%, depending on the area studied. Bacteria were significantly less frequently detected in nymphs - 2.1% (5/235) than in adult ticks - 13.6% (44/324) and in males - 4.2% (74/165) than in females - 23.3% (37/159) (p ? 0.05). The obtained results confirm both the occurrence of HA foci in north-eastern Poland with I. ricinus as the principal vector of the A. phagocytophilum infection, and forestry workers as the main group at risk.

Risk of tick-borne bacterial diseases among workers of Roztocze National Park [South-Eastern Poland]

67%

Cisak E., Chmielewska-Badora J., Zwolinski J., Wojcik-Fatla A., Polak J., Dutkiewicz J.

Annals of Agricultural and Environmental Medicine

2005

tom 12

nr 1

The objective of the present study was to assess the risk of borreliosis and anaplasmosis (ehrlichiosis) among the forestry workers of the Roztocze National Park (south-eastern Poland) by examination of Ixodes ricinus ticks living in park area with PCR method, and by the serological and clinical examination of the workers. In 406 examined ticks, the prevalence of infection with Borrelia burgdorferi sensu lato was 11.5%. The nested PCR reaction for determining the genospecies showed that the most common was Borrelia burgdorferi sensu stricto (55.3% of total positive) followed by Borrelia afzelii (38.3%). As many as 6.1% out of 115 examined ticks showed the presence of Anaplasma phagocytophilum DNA. The infection rate was high in males and females (14.3% and 11.1% respectively) and low in nymphs (1.5%). In 46 out 113 examined forestry workers (40.7%) the presence of specific IgG and/or IgM antibodies against Borrelia burgdorferi sensu lato was found in ELISA test, while only 4 out of 56 urban blood donors showed a positive response (p<0.0001).The prevalence of IgG antibodies against Anaplasma phagocytophilum determined with the use of indirect immunofluorescence test (IFA) was 17.7% in forestry workers compared to 5.4% in reference group of blood donors (p<0.05). No correlation was found between the presence of antibodies to A. phagocytophilum and to B. burgdorferi s. l. Clinical investigations of 113 forestry workers showed 3 cases of borreliosis (2.7%) and no cases of anaplasmosis (ehrlichiosis). In conclusion, forestry workers of the Roztocze National Park in south-eastern Poland are often exposed to Ixodes ricinus ticks infected with Borrelia burgdorferi and Anaplasma phagocytophilum, and show a high proportion of asymptomatic borreliosis and anaplasmosis (ehrlichiosis) manifested by a positive serologic response, while the number of clinical cases is relatively low.

Ograniczanie wyników

2 Annals of Agricultural and Environmental Medicine

1 Chmielewska-Badora J.

1 Cisak E.

1 Dutkiewicz J.

1 Grzeszczuk A.

1 Kruminis-Lozowska W.

1 Kubica-Biernat B.

1 Polak J.

1 Prokopowicz D.

1 Racewicz M.

1 Stanczak J.

1 Wojcik-Fatla A.

1 Zwolinski J.

1 2005

1 2004

Wyniki wyszukiwania

Human anaplasmosis in North-Eastern Poland: seroprevalence in humans and prevalence in Ixodes ricinus ticks

Risk of tick-borne bacterial diseases among workers of Roztocze National Park [South-Eastern Poland]