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Textile floor coverings (TFC), an important element of equipment in homes and public buildings, are regarded as a settlement of house dust mites, and thus the cause of mite-induced allergy. It is believed that TFC should be removed from premises used by persons with a diagnosed allergy to house dust mites. On the other hand, TFC removal is associated with an increased air concentration of dust particles and dust-containing allergens. It is feasible to equip TFC with properties that can prevent the settlement and development of dust mites. The aim of this study was to evaluate TFC properties designed to protect against dust mite generation. The culture of Dermatophagoides farinae was used to test the efficacy of the direct anti-mite action. TFC acquired anti-dust mite properties through the addition of biologically active agents to the coated backing and mounting the same acaricides and anti-adhesive fluorocarbon dispersion on pile fibers. The results of our studies show that modified TFCs are characterized by anti-dust mite properties that protect against Dermatophagoides farinae.
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House dust is an important source of different toxic metabolites as well as allergens, including those of fungal origin, in the indoor environment. A bio-assay employing 1-day-old chick tracheas was used to characterize airway effects of 2-butanone and dimethylsulphoxide (Me2SO) extracts of 23 dust samples collected from water damaged (13) and control (10) Danish schools. Direct microscopical analysis of samples, followed by cultivation on dichloran 18% glycerol agar at 25oC for 10 days to establish their mycoflora, was performed. The in vitro ciliostatic potential of the chloroform-extractable endo- and exometabolites of 41 representative fungal isolates was determined. Nine dust extracts in 2-butanone (2 from damp rooms) or 10 (6) in Me2SO showed some ciliostatic activity in the 3-days' experiment. Fungal composition of dust from buildings with leakage was almost identical with that from undamaged houses, as well as the fungal colony counts from the damp schools and the control samples. Aspergillus spp. were prevalent in the samples - 31 or 40% of all fungi, followed by Penicillium spp. and Cladosporium cladosporioides. Alternaria spp., Chaetomium spp., Mucor spp., Mycelia sterilia, Paecilomyces variotii, Rhizopus sp., Ulocladium sp. and yeasts were each isolated in less than 8% of the fungal content. No Aspergillus flavus isolate (8 in total) was aflatoxigenic ,em>in vitro. Alternaria spp., Aspergillus spp., Botrytis cinerea, Penicillium spp., C. cladosporioides, Chaetomium spp. and Ulocladium sp.; in total, 88% of all fungi tested, produced ciliostatically active metabolites. These toxigenic strains were also present in 4 dust samples from controls and 5 dust samples from water damaged buildings. Extracts of these dust samples were also toxic in bioassay. There were bio-detectable concentrations (10-20 µg of extracts/ml of the organ culture medium) of toxic compounds in house dust. Contribution of fungal metabolites to its toxic effect should be studied further.
The polarized response of T helper-2 (Th2) lymphocytes to an allergen is considered to be the main cause of the pathogenesis of asthma. In this study, we asked a question whether M. bovis BCG mycobacteria which are known for the preferential stimulation of T helper-1 (Th1) immunity, diminish the effector functions of Th2 cells from allergic patients upon stimulation with a common house dust mite Der p-1 allergen. Our results allow a positive answer to this question. We demonstrate that BCG modulates the dendritic cell-dependent allergen presentation process and switches naive T lymphocytes towards an anti-allergic Th1 profile.
The aim of this study was to evaluate the frequency of positive skin prick test (SPT) results to house dust mite (HDM) allergens - perennial inhalant allergens. Material and methods: The study was carried out on 2197 patients of either sex aged 20 - 45 (mean age 32.4 ± 0.1 years). All patients enrolled in this study were allergic to house dust mites. Results: In all patients the following clinical manifestations were found: positive skin (SPT) tests to house dust mite (HDM) allergens. The following allergens were the most common cause of allergic rhinitis in patients admitted to the Clinic of Allergology in the period 1995-2002: Dermatophagoides pteronyssinus, Dermatophagoides farinae, Acarus siro, Lepidoglyphus destructor, Tyrophagus putrescentiae.
Agricultural work is considered to be a major risk factor for occupational diseases. In particular, allergic reactions to cow dander cause numerous cases of airway disorders. We measured the concentration of allergens (e.g. Bos d2, Der p1) and endotoxin in the stables, living-rooms and mattresses of 46 farmers with a diagnosis of occupational asthma or allergic rhinitis caused by cow dander allergen. The concentration of cow dander allergen was highest in stables (median 20,400 µg/g) but also noticeable in dust samples from living-rooms (median 155 µg/g) and mattresses (median 195 µg/g). The sensitization threshold (20-50 µg/g) was exceeded in most cases. Thus, allergen transport from the stables to bed must be prevented by optimizing the hygiene of farmers and family members.
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