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The present paper describes the results of induction spermiation and ovulation of ide (Leuciscus idus L.) during and out of spawning season using CPE with addition of hCG, hCG and ovopel - GnRH containing pellets. Males from control group produced significantly smaller volume of milt (season) than those from hormonally treated groups. Significant differences in spermatozoa motility was noted between treated and control males. The percent of ovulated females from groups treated with CPE and ovopel was 100%. Fish from control groups and treated with hCG did not ovulate. The total spawners mortality was quite high, especially in fish treated with ovopel. Generally, all recorded parameters in out of spawning season were lower than those obtained during artificial propagation in natural spawning time.
Spermiation and ovulation in the Eurasian perch Perca fluviatilis L. were induced with Ovopel, pellets containing GnRH, and then compared with hCG and CPE. Males from the control group produced a significantly smaller volume of milt (average about 17mlkg BW⁻¹) than those from the hormonally-treated groups (over 24 ml kg BW⁻¹). Spermatozoa motility was similar in both the treated and untreated males, but the fertilization ability varied significantly between individual males. Over 90% of the treated females ovulated. Ovulation in the control group was noted in a limited number of females. Mean embryo survival to the eyed-egg-stage was over 50% in the treated groups, in contrast to 7.5% in the control group. The poor quality of perch gametes might result from high water temperature oscillations before the natural spawning season. The quality of eggs, expressed as survival to the eyed-egg-stage, decreased with latency time.
This study determined the effect of hormonal stimulation of the wild female nase, Chondrostoma nasus (L.), on its basic reproduction indices (percentage of ovulations, latency time, embryo survival) and the economic profitability of its use. Two commercial preparations were used in the experiment: Ovopel and Ovaprim. They were used separately (group 1 and 2 for Ovopel and Ovaprim, respectively) and in combination (group 3), where Ovopel was given in initial and Ovaprim in resolving injection. The study found a high effectiveness of all the hormonal treatments applied (ovulation rate 90–100%, latency time 36 h, embryo survival rate 78.6–81.2%) (P>0.05), which may be evidence of the greater susceptibility of the nase to stimulation with the less active mammalian analogue of GnRH as compared to other species of rheophilic cyprinids. In consequence, the lowest cost of hormonal stimulation (0.59 EUR per 10,000 viable embryos) was achieved with Ovopel. Using the hormonal agents in combination (in group 3) reduced the cost of stimulation by 0.17 EUR as compared to Ovaprim (group 2), where the cost was the highest (1.57 EUR per 10,000 viable embryos). The results presented in this study are providing useful information for fish breeders who manage wild populations of the nase and other species of rheophilic cyprinids.
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