We analysed the effects of [(D-Ala⁶, Pro⁹NEt)-mGnRH+metoclopramide] i.e. Ovopel at the dose of 0.5 granule kg⁻¹ of body weight (b.w.), [(D-Arg⁶, Pro⁹NEt)-sGnRH+domperidone] i.e. Ovaprim at the dose of 0.25 ml kg⁻¹ b.w., luteinizing hormone releasing hormone (LHRHa) at the dose of 50 μg kg⁻¹ b.w. and carp pituitary extract (CPE) at the dose of 2 mg kg⁻¹ b.w. on the total volume of milt (TVM, ml), volume of milt per kg of body weight (VOM, kg⁻¹ b.w.), spermatozoa motility [%], osmolality of seminal plasma (mOsm kg⁻¹), spermatozoa concentration (×10⁹ ml⁻¹), total sperm produced (TSP, ×10⁹) and total number of spermatozoa per kg of b.w. (TNS, ×10⁹ kg⁻¹ b.w.) in the chub Leuciscus cephalus (L.). Milt was collected 24 hours after injection from individuals (n=9) belonging to each group. The control group (control, n=9) consisted of the males which were given 0.9% NaCl at 0.5 ml kg⁻¹ b.w. The highest values of TVM and VOM were observed after Ovaprim (5.88±1.62 ml and 19.24±3.56 ml kg⁻¹ b.w. respectively) and CPE stimulation (5.39±1.19 ml and 19.61±3.32 ml kg⁻¹ b.w. respectively). The lowest TVM and VOM values were observed after LHRHa injection (2.46±0.89 ml and 8.95±3.13 ml kg⁻¹ b.w. respectively) and those values were statistically different from values recorded after Ovopel (P<0.05), Ovaprim (P<0.001) and CPE (P<0.001). A significant increase in spermatozoa motility (P<0.05) was observed following hormonal stimulation as compared to the control, regardless of the type of hormonal agent applied. The values of osmolality were similar in all the groups with no significant differences between them (288–300 mOsm kg⁻¹, P>0.05). The highest spermatozoa concentrations in the control were 10.26±1.70 · 10⁹ ml⁻¹, and the lowest values were 5.47±0.99 · 10⁹ ml⁻¹, P<0.001, observed after Ovaprim stimulation. The highest TSP and TNS values were recorded after CPE injection (42.84±11.72 · 10⁹ and 160.4±65.67 · 10⁹ kg⁻¹ b.w. respectively), while the lowest were obtained following stimulation with LHRHa (23.57±8.56 · 10⁹ and 85.72±30.56 · 10⁹ kg⁻¹ b.w. respectively). Those differences were statistically significant (P<0.05). Considering the large volume of milt (TVM and VOM) and the large number of spermatozoa (TSP and TNS), chub spermation can be successfully stimulated with Ovaprim or CPE injections.
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