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1

Ancylostoma ceylanicum metalloprotease 6 DNA vaccination induces partial protection against hookworm challenge infection

100%
Wisniewski M., Jaros S., Baska P., Cappello M., Wedrychowicz H.
Acta Parasitologica
|
2013
|
tom 58
|
nr 3
Hookworms are blood feeding intestinal nematodes that infect more than 500 million people and cause iron deficiency anemia. Infected children suffer from physical and cognitive growth retardation. Because of potential anthelminthic drug resistance, the need for vaccine development is urgent. Numerous antigens have been tested in animal models as vaccines against hookworm infection, but there is no effective human vaccine. We cloned a cDNA encoding Ancylostoma ceylanicum metalloprotease 6 (Acemep-6). Ace-MEP-6 is a protein with a predicted molecular mass of 101.87 kDa and based on computational analysis it is very likely to be engaged in food processing via hemoglobin digestion. Groups of hamsters were immunized with an Ace-mep-6 cDNA vaccine, either once or three times. Animals that were administered one dose developed high resistance (80%, p < 0.01) against challenge infection, whereas triple immunization resulted in no worm burden reduction. These results suggest that DNA vaccines can be powerful tools in ancylostomiasis control, although the mechanisms through which protection is conferred remain unclear.
2

Vaccination with a cocktail of Ancylostoma ceylanicum recombinant antigens leads to worm burden reduction in hamsters

84%
Wisniewski M., Lapinski M., Danilowicz-Luebert E., Jaros S., Dlugosz E., Wedrychowicz H.
Acta Parasitologica
|
2016
|
tom 61
|
nr 3
3

Cloning and molecular characterization of cDNAs encoding three Ancylostoma ceylanicum secreted proteins

84%
Siwinska A. M., Baska P., Danilowicz-Luebert E., Januszkiewicz K., Dlugosz E., Wedrychowicz H., Cappello M., Wisniewski M.
Acta Parasitologica
|
2013
|
tom 58
|
nr 1
Ancylostoma ceylanicum belongs to a group of soil-transmitted helminths, which infect almost 576 mln people worldwide and are a major cause of anaemia and malnutrition. Upon contact with a permissive host, third-stage larvae (L3) residing in the environment become activated larvae (ssL3), a process associated with changes in the profile of gene expression. Ancylostoma secreted proteins (ASPs) are the major proteins secreted during larvae activation and play a crucial role in hookworm adaptation to parasitism. Here we report the cloning using RACE-PCR technique of three novel ASPs from the hookworm A. ceylanicum (Ace-asp-3, Ace-asp-4, and Ace-asp-5) and computational analysis of the protein sequences. All three proteins contain SCP (Sperm Coating Protein) domain characteristic for previously described ASP proteins. Real-time PCR analysis shows significant up-regulation of Ace-asp-3 and Ace-asp-5 expression in adult worms and correlated down-regulation in ssL3 larvae. On the other hand, expression of Ace-asp-4 was increased in ssL3 stages and decreased in adult parasites.
4

Helminth and respiratory mite lesions in Pinnipeds from Punta San Juan, Peru

84%
Seguel M., Calderon K., Colegrove K., Adkesson M., Cardenas-Alayza S., Paredes E.
Acta Parasitologica
|
2018
|
tom 63
|
nr 4
5

Mast cell, eosinophil and IgE antibody response of BALB-c mice to percutaneous infection with the canine hookworm Uncinaria stenocephala

84%
Gorski P., Krawiec M., Behnke J. M., Wedrychowicz H.
Acta Parasitologica
|
1999
|
tom 44
|
nr 3
The host-parasite relationship of the post-infection tissue resident and migratory stages of Uncinaria stenocephala are areas, which have received very little attention to-date. In the present experiments cellular and serum antibody responses were evaluated in mice infected percutaneously with infective larvae of the nematode. Significant eosinophil infiltration was observed in the skin at the site of infection. The number of these cells increased significantly (p<0.01) and dramatically at the site of the first infection (abdomen) within 24 h of exposure to the second dose of larvae which was administered at a different site (back). A clear IgE response of mice to somatic and surface antigens of L3 was observed. There was no further increase in IgE to the somatic antigen preparations following challenge, but a significantly higher concentration of IgE reactivity to surface antigens was detected 14 days after challenge. A short-lived, IgM, IgG and IgA response to the somatic antigens was also detected.
6

Vaccination of rats against the rodent hookworm Nippostrongylus brasiliensis with a recombinant superoxide dismutase fails to protect against infection

67%
Ball G., Knox D. P.
Acta Parasitologica
|
2009
|
tom 54
|
nr 3
281-287
Anti-oxidant enzymes including superoxide dismutase (SOD) protect cells from damage by oxygen radicals produced during respiration. There is also a substantial body of evidence that anti-oxidant enzymes facilitate the survival of parasitic helminths, including gastrointestinal nematodes, within the host. Superoxide dismutase has been shown to be released by a variety of parasitic helminths and may protect them from host mediated oxidative immune responses. As it may play a parasite protective role during infections SOD has been investigated as a vaccine candidate in a range of helminth parasites including Schistosoma mansoni, Acanthocheilonema viteae and Haemonchus contortus. Here, we sought to evaluate the protective potential of SOD against the rat hookworm Nippostrongylus brasiliensis, a commonly utilised laboratory infection, as a vaccination model. A cytosolic SOD from this parasite, with high sequence homology to a putative extracellular form of the enzyme was cloned and then expressed in bacteria. The resultant recombinant protein was assessed for enzyme activity and used to immunise rats prior to a single challenge infection with the parasite. No protection was observed and monitoring systemic and mucosal antibody responses and mast cell protease levels in superoxide dismutase vaccinated rats suggested that this recombinant SOD was only weakly immunogenic.
7

Differentiation of two dog hookworm species using PCR

67%
Mieszczanek J., Wedrychowicz H.
Acta Parasitologica
|
1999
|
tom 44
|
nr 1
Reliable determination of hookworm nematode parasite species from dogs can be carried out post-mortem by microscopical examination of the buccal cavity of adult worms isolated from the host small intestine. In order to allow a proper diagnosis of the hookworm infection in a living host, we developed a polymerase chain reaction (PCR). Using DNA extracted from hookworm eggs and an oligonucleotide set designed on the basis of the DNA sequence of a cysteine proteinase AcCP1 gene, Ancylostoma caninum and Uncinaria stenocephala DNA can be discriminated from each other.
8
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Cloning of cDNA of the cysteine proteinase gene from Ancylostoma ceylanicum - a potential vaccine antigen

67%
Mieszczanek J., Wedrychowicz H.
Wiadomości Parazytologiczne
|
1998
|
tom 44
|
nr 3
9
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Hookworm infections in human and laboratory animals - differences and similarities in immune responses

67%
Dlugosz E.
Wiadomości Parazytologiczne
|
2005
|
tom 51
|
nr 3
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