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Proteinase inhibitors from squash seeds were analyzed for mutational variability. The non-homologous positions were subjected to an analysis of the interrelation between occurring residues and the mechanism of variability, using the algorithm of genetic semihomology [1]. The study also concerned mutational correlation at particular positions and their contact with each other. It was observed that: the number of residues occupying particular positions varies from 1 to 8 the mechanism of variability is based on single point mutation the variable positions are seldom in contact with each other the mutations in distant positions (not in contact with each other) are correlated with each other the correlated mutations refer to those positions which are far from the reactive site of the inhibitor the mutational variability in primary structure within this family is not consistent with the Markovian model of amino acid replacement.
Systematics of fossil octodontoids (Rodentia, Caviomorpha) is in great part based on insights into the knowledge of teeth, making the step of dental characterization certainly relevant for the evolutionary reconstruction of these rodents. Different homology hypotheses were proposed for the same tooth structures, a fact that indicates the importance of knowing on which criteria the dental characters supporting the classifications were based. In this line, I evaluate the step of characterization of certain conflictive molar characters previously used, and their impact on phylogeny of octodontoids. I explore which the criteria followed to propose the hypotheses of correspondences for these characters are in light of the anatomical evidence. Based on the outcome of phylogenetic trees obtained previously, I analyze if the evolutionary transformations are compatible with character states observed in the terminals. New cladistic analyses based on recoded molar characters indicate that, unlike results recently obtained, the unorthodox position of Sallamys, Protadelphomys, and Willidewu as basal ctenomyines is not recovered. The position of Caviocricetus, Acarechimys–Neophanomysas as Octodontinae is not maintained. These results indicate that reanalyses of conflictive dental characters, scrutinizing data matrices, are particularly necessary to evaluate the current controversy on the phylogeny of octodontoids. Lower molar character definition and character states delimitation in octodontoids, being relevant to phylogenetic reconstruction, should be founded on anatomical examination, following explicit criteria of homology. Alternative hypotheses of “primary homology” proposed for the same molar traits in octodontoids indicate that each main group of caviomorphs requires its own anatomical study.
Cusp and lophid homologies of the lower deciduous teeth (dp4) in erethizontids and other Hystricognathi are specified. On this basis, a new nomenclature for these structures is proposed. The probable primitive condition and evolution of the occlusal patterns of these teeth are also analyzed. In contrast to previous proposals, it is concluded that the mesoconid, mesostylid, and mesolophid of the dp4 of erethizontids can be recognized since the Early Miocene. The anteriormost three lophids of the pentalophodont dp4 of the Erethizontidae would be homologous to the anterolophid, metalophulid II, and mesolophid, respectively. In addition, it may be proposed that the lophids of the dp4 of the Baluchimyinae and Old World Hystricognathi are homologous to those of the erethizontids and the remaining South American Hystricognathi. The pentalophodont pattern is probably the primitive condition of the dp4 of the Hystricognathi.
Polymorphism of upper molar (M1) crown patterns of Taxidea taxus (Schreber, 1777) involves the number, position and interconnection of the cones and cristae constituting the inner part of the crown. Some questions of homology of the cones are briefly considered.
A 5.4 kb BamHl fragment of R. leguminosarum bv. trifolii TA1 was found to carry genes involved in exopolysaccharide synthesis (caro genes). This fragment was strongly hybridized to the total DNA from R. I. bv. viciae and bv. phaseoli digested with EcoRI. No homology was found with total DNA of R. meliloti and Rhizobium sp. NGR 234. The exo genes from R. I. bv. trifoliiTAl conjugally introduced into R. I. bv. viciae 1302 considerably affected the symbiosis: the nodules induced on vetch were abortive and did not fix nitrogen. On the other hand, Phaseolus beans infected with R. I. bv. phaseoli harbouring R. I. bv. trifolii exo genes formed the nitrogen-fixing nodules. It can be concluded that additional copies of exo genes introduced into wild type Rhizobium leguminosarum strains can disturb the synthesis of acidic exo- polysaccharides and affect symbiosis of the plants forming indeterminate nodules, but do not affect symbiosis of the plants forming the determinate nodules.
The RYR1 gene encoding the Ca²⁺ channel of sarcoplasmic reticulum of human skeletal muscle has been cloned and its nucleotide sequence has been determined earlier. We have used the polymerase chain reaction single strand conformation polymorphism (PCR-SSCP), and sequencing analysis for human, porcine (Sus scrofa), and zebrine (Equus grevyi) ryanodine receptor (ryrl) gene. The fragment of exon 17 of the ryr1 gene was characterized by a high homology between all the analysed species (substitution of a nucleotide is underlined): porcine ryr1 ¹⁸³⁴GTG GCC GTG CGC TCC AAC CAA GAT CT¹⁸⁵⁹ human RYR1 ¹⁸³¹GTG GCC GTG CGC TCC AAC CAA GAT CT¹⁸⁵⁶ zebrine ryr1 GTG GCC GTG CGC TCC AAC CAA GAC CT.
The isolation of rye ß-Amy1 and ß-Amy2 gene promoters from nuclear DNA using the inverse polymerase chain reaction (IPCR) technique and characterization of their sequences are presented. The conservation of ß-amylasc coding sequences allowed for simultaneous IPCR amplification of two different promoters with primers designed on the basis of the single known cDNA sequence. Two ß-amylasc gene promoters display a low sequence similarity (47%). Beside consensus TATA and CCAAT boxes, other sequence motives common to both promoters were found. In addition, the homology of amino acid sequences of plant ß-amylases available in the database is discussed.
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