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Neurogenic inflammation is linked to urinary bladder overactivity development. Cyclophosphamide (CYP) damages all mucosal defence lines of urinary bladder and induces cystitis with overactivity. The aim of this study was to estimate the effect of CYP on rat urinary bladder function, histological structure and mastocytes numbers following acute and chronic CYP treatment. Fourty two female rats were divided into four groups: I (control), II (acute cystitis), III (chronic cystitis), IV (sham group). Acute and chronic cystitis were induced by CYP in single dose and four doses (1st, 3rd, 5th, 7th day), respectively. In group I–III the cystometric evaluation was performed. Sections of the bladder were stained with HE and toluidine blue for the detection of mastocytes. The severity of inflammation was examined according to mucosal abrasion, haemorrhage, leukocyte infiltration and oedema. Acute and chronic CYP treatment caused inflammatory macroscopic and microscopic changes (mucosal abrasion, haemorrhage, oedema) and increased infiltration of inflammatory cells in urinary bladder. Acute treatment induced the infiltration of mastocytes within bladder wall contrary to chronic one decrement. Acute treatment caused more severe mucosal abrasion, whereas chronic one revealed more developed haemorrhage changes. Additionally, cystometric evaluation revealed urinary bladder overactivity development in both types of cystitis. Basal pressure and detrusor overactivity index after acute treatment increased considerably in comparison with the increase obtained after chronic one. Our results proved that acute model of CYP-induced cystitis in rats is more credible for further evaluation of neurogenic inflammation response in pathogenesis of overactive bladder as compared to chronic one.
The objective of the presented study was to assess the dynamics of changes of the histological parameters of the following two pork muscles: the semimembranosus muscle (musculus semimembranosus) and the quadriceps muscle of the thigh (musculus quadriceps femoris) under the influence of curing, massaging and pasteurisation processes with the assistance of the computer image analysis. The analysis of the muscle structure was conducted on the basis of: the surface and circumference of muscle fibres, Feret’s H and V diameter, percentage proportion of muscle fibres and their quantity in the analyzed field of view. The fibre shape was determined on the basis of the H/V ratio. It was found that the quadriceps muscle of the thigh exhibited greater dynamics of changes of the structure elements in comparison with the semimembranosus muscle under the influence of injection and plasticizing processes. The injection of ham muscles resulted in the loosening of muscle fibres, rounding of their contours as well as in the increase of their cross section area. The pasteurisation process failed to cause changes in the structure of the quadriceps muscle of the thigh, whereas in the semimembranosus muscle, pasteurisation increased the cross section area and circumference of muscle fibres and their percentage proportion in the examined field of view in comparison with the massaged muscle. The applied pasteurisation process was found to even out cell dimensions and to decrease inter-cellular spaces in both of the examined muscles.
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