Wyniki wyszukiwania

1

Measurement of nitric oxide in hippocampal slices: induction with nitroso compounds and the effect of depolarization

100%

Haklar C., Saybasili H., Yuksel M., Yalcin A. S.

Acta Neurobiologiae Experimentalis

|

2003

|

tom 63

|

nr 4

2

GABAergic and glutamatergic currents in hippocampal slices and neuronal cultures show profound differences: a clue to a potent homeostatic modulation

84%

Szczot M., Wojtowicz T., Mozrzymas J. W.

Journal of Physiology and Pharmacology

|

2010

|

tom 61

|

nr 4

501-506

Acute hippocampal slices and primary neuronal cultures are often used with a tacit assumption that basic characteristics of the two models closely resemble each other. The use of the cell cultures, however, may raise controversies because of non-physiological conditions resulting from e.g. glial cells deficit, random neuronal sprouting, lack of specificity in the synaptic connections, impaired homeostasis, etc. Importantly, alteration in neuronal environment, especially when occurring over a prolonged period of time, may give rise to a profound homeostatic modulation. In the present study we have compared the properties of GABAergic and glutamatergic (non-NMDA) currents in pyramidal neurons from hippocampal slices and neuronal cell culture. We show that, most strikingly, amplitude ratio of currents elicited by ultrafast applications of saturating GABA and glutamate was nearly one order of magnitude larger in cultured neurons than that in slices. Miniature IPSCs and EPSCs also showed substantial differences between these two models. In particular, mEPSC amplitudes were larger and more frequent in cultured neurons but their time duration was longer in slices. Miniature IPSCs did not show differences in amplitude when comparing slices and cultures but their time duration was faster and occurrence more frequent in slices. In conclusion, we provide evidence that expression pattern of GABAA and glutamate receptors as well as synaptic current properties in the neuronal cell culture show profound differences with respect to that in the physiological conditions.

3

5-HT7 receptors modulate GABAergic transmission in rat hippocampal CA1 area

67%

Tokarski K., Kusek M., Hess G.

Journal of Physiology and Pharmacology

|

2011

|

tom 62

|

nr 5

4

The effect of glutamate uptake inhibitors on hippocampal evoked potentials in vitro

67%

El-Sherif Y., Singh N., Khan T., Wieraszko A.

Acta Neurobiologiae Experimentalis

|

1999

|

tom 59

|

nr 2

5

Phosphorylation of protein kinase C substrate proteins in rat hippocampal slices-effect of calpain inhibition

67%

Domanska-Janik K., Zablocka B., Zalewska T., Zajac H.

Acta Neurobiologiae Experimentalis

|

1998

|

tom 58

|

nr 4

Ograniczanie wyników

3 Acta Neurobiologiae Experimentalis

2 Journal of Physiology and Pharmacology

1 Domanska-Janik K.

1 El-Sherif Y.

1 Haklar C.

1 Hess G.

1 Khan T.

1 Kusek M.

1 Mozrzymas J. W.

1 Saybasili H.

1 Singh N.

1 Szczot M.

1 Tokarski K.

1 Wieraszko A.

1 Wojtowicz T.

1 Yalcin A. S.

1 Yuksel M.

1 Zablocka B.

1 Zajac H.

1 Zalewska T.

1 2011

1 2010

1 2003

1 1999

1 1998

Measurement of nitric oxide in hippocampal slices: induction with nitroso compounds and the effect of depolarization

GABAergic and glutamatergic currents in hippocampal slices and neuronal cultures show profound differences: a clue to a potent homeostatic modulation

5-HT7 receptors modulate GABAergic transmission in rat hippocampal CA1 area

The effect of glutamate uptake inhibitors on hippocampal evoked potentials in vitro

Phosphorylation of protein kinase C substrate proteins in rat hippocampal slices-effect of calpain inhibition