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Kernel size and morphology influence the market value and milling yield of bread wheat (Triticum aestivum L.). The objective of this study was to identify quantitative trait loci (QTLs) controlling kernel traits in hexaploid wheat. We recorded 1000-kernel weight, kernel length, and kernel width for 185 recombinant inbred lines from the cross Rye Selection 111 × Chinese Spring grown in 2 agro-climatic regions in India for many years. Composite interval mapping (CIM) was employed for QTL detection using a linkage map with 169 simple sequence repeat (SSR) markers. For 1000-kernel weight, 10 QTLs were identified on wheat chromosomes 1A, 1D, 2B, 2D, 4B, 5B, and 6B, whereas 6 QTLs for kernel length were detected on 1A, 2B, 2D, 5A, 5B and 5D. Chromosomes 1D, 2B, 2D, 4B, 5B and 5D had 9 QTLs for kernel width. Chromosomal regions with QTLs detected consistently for multiple year-location combinations were identified for each trait. Pleiotropic QTLs were found on chromosomes 2B, 2D, 4B, and 5B. The identified genomic regions controlling wheat kernel size and shape can be targeted during further studies for their genetic dissection.
Tibetan semi-wild wheat (Triticum aestivum ssp. tibetanum Shao) is one of the Chinese endemic hexaploid wheat genetic resources, distributed only in the Qinghai-Xizang Plateau of China. It has special characters, such as a hulled glume and spike disarticulation. However, seed dormancy, another important character for wheat resistance to pre-harvest sprouting, was rarely reported. Seed dormancy of more than 10 Tibetan semi-wild wheat accessions was evaluated, and their germinations were 0% or near 0% with both treatments of threshed seeds and intact spikes at hard dough stage. Tibetan semi-wild wheat accession Q1028 was investigated for its seed dormant characters by testing the seed germination percentages of intact spikes, seeds with bract powder, normal seeds, seeds with pierced coat, and sectioned embryos. It was observed that embryo dormancy of Q1028 accounted for its seed dormancy. Using threshed seeds and intact spikes at hard dough stage, the inheritance of seed dormancy was carried out using the F₁, F₂, F₃ and F₂BC₁ populations of the cross between Q1028 and a wheat line 88-1643, susceptible to preharvest sprouting. The germinations of seeds and intact spikes in F₁ plants were 1.0% and 0.9%, respectively. It indicated that seed dormancy of Q1028 was inherited as a dominant trait. From the genetic analysis of the F₂, F₃ and F₂BC₁ populations it was found that the strong seed dormancy of Q1028 was controlled by two dominant genes.
The brittle rachis character, which causes spontaneous shattering of spikelets, has an adaptive value in wild grass species. The loci Br₁ and Br₂ in durum wheat (Triticum durum Desf.) and Br₃ in hexaploid wheat (T. aestivum L.) determine disarticulation of rachides above the junction of the rachilla with the rachis such that a fragment of rachis is attached below each spikelet. Using microsatellite markers, the loci Br₁, Br₂ and Br₃ were mapped on the homoeologous group 3 chromosomes. The Br₂ locus was located on the short arm of chromosome 3А and linked with the centromeric marker, Xgwm32, at a distance of 13.3 cM. The Br₃ locus was located on the short arm of chromosome 3B and linked with the centromeric marker, Xgwm72 (at a distance of 14.2 cM). The Br₁ locus was located on the short arm of chromosome 3D. The distance of Br₁ from the centromeric marker Xgdm72 was 25.3 cM. Mapping the Br₁, Br₂, and Br₃ loci of the brittle rachis suggests the homoeologous origin of these 3 loci for brittle rachides. Since the genes for brittle rachis have been retained in the gene pool of durum wheat, the more closely linked markers with the brittle rachis locus are required to select against brittle rachis genotypes and then to avoid yield loss in improved cultivars.
The set consisting of 42 forms of Triticum tauschii (syn. Aegilops squarrosa) Coss. gathered in the National Small Grains Collection (USA) and 4 forms from the collection of the Institute of Plant Genetics PAS was investigated in terms of resistance to powdery mildew (Erysiphe graminis f. sp. tritici) under the artificial inoculation conditions. Out of all studied forms, 21 collection forms (45.6%) expressed resistance at the seedling stage and at the stage of vegetative development, but only in 12 forms (26%) resistance was expressed at the fully developed stage. Resistant forms formed clusters in terms of their origin from specific geographical regions. Under the infection promoting conditions, hexaploid wheat cultivars Igna, Omega and Tercja were already distinctly infected at the early development stages and were strongly infected at the fully developed stage. Two completely resistant forms of T. tauschii Coss. (151 PI, 152 PI) were crossed with a susceptible wheat cultivar Igna to introduce genetic material directly from genome Dt carrying resistance genes (omitting the generation of a synthetic hexaploid). The previously described crossing efficiency using T. tauschii Coss. as a maternal form was confirmed. Embryos from the non-endospermic hybrid cary-opses were cultured in vitro. The character limiting the potential of generating F₁ plants was high susceptibility of embryos to callus formation even at their considerably advanced developmental stages. Out of the produced F₁ plants as a result of the colchicine treatment allooctoploid genotypes were generated with the ABBDDDtDt genome, which may be used as bridging forms for the introgression of resistance genes into selected wheat cultivars.
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