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110 blood samples from clinically healthy mares of English breed, half-blood and the Wielkopolska race were tested for the presence of EHV-1 and EHV-2 using nested PCR. 15 samples were EHV-1-positive, 24 samples were EHV-2-positive, whereas only 4 samples were both EHV-1 and EHV-2 positive. The virus was isolated from PBLs in equine dermal cell cultures by co-cultivation or by culture inoculation with cell lysates derived from PBLs. A total of 14 strains were isolated from EHV-2 and EHV-1/EHV-2-positive samples. However, all of them were identified by nPCR as being type 2. Since no EHV-1 was isolated, even from dually infected leukocytes, it was concluded that the presence of EHV-2 does not stimulate in-vitro isolation of EHV-1 from infected leukocytes. It is tempting to speculate that such stimulatory effects in-vivo may involve EHV-2-induced immunosupression. Despite any possible mechanism of EHV-1 stimulation, it seems that EHV-2 does not play a significant role in the epidemiology of EHV-1-caused miscarriages in horses since mixed infections are rather rare.
Equine herpesvirus 1 (EHV1) is one of the most important infectious agents in horses. This virus causes inflammation of the upper respiratory tract, pneumonia, abortion, death of newborn foals and encephalomyelitis known as Equine Herpesvirus Myeloencephalopathy (EHM). In recent years there has been a marked increase in the incidence of EHM caused by infection with neuropathogenic strains of EHV1. For this reason, some experts believe that EHM should be classified as a newly emerging infectious disease. Although this disease is less frequently observed than the other clinical forms of EHV1 infection, it may cause serious economic losses in breeding horses and have a very negative impact on the functioning of riding schools, racetracks and veterinary hospitals. This review discusses selected aspects of EHM, such as the link between the neurologic form of the disease and the EHV1 genotype, clinical signs, and methods of diagnosis and prevention.
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