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The Shiga toxin-producing Escherichia coli (STEC) strains are currently considered important emerging pathogens threatening public health. Among Shiga toxin-producing Escherichia coli, E. coli O157:H7 strains have emerged as important human pathogens. This study was conducted to determine the presence of Escherichia coli O157 and O157:H7 in raw milk samples and Van herby cheese samples. For this purpose, 100 samples of raw milk were collected and 100 samples of herby cheese sold for consumption in Van province in Turkey were obtained from grocers and markets in order to detect the presence of Escherichia coli O157 and O157:H7. The method of E. coli O157 and O157:H7 isolation proposed by the Food and Drug Administration (FDA) was used. E. coli O157 in raw milk and herby cheese samples was found in 11% and 6% of samples respectively, and E. coli O157:H7 was found in 2% of herby cheese samples. No E. coli O157:H7 was detected in raw milk samples. This study showed that raw milk was contaminated with E. coli O157 and herby cheese was contaminated with both E. coli Ol 57 and E. coli Ol57:H7; therefore, herby cheese poses a serious risk to public health.
Staphylococcus aureus reference strains, producing A, B, C, and D enterotoxins, were individually or as mixture inoculated at the rate of 10⁵ cfu/mL into 10 herby cheese samples, experimentally produced from pasteurised or raw milk. The growth rates and enterotoxin production abilities of the strains were examined during the 90 d ripening period. During the ripening, S. aureus, aerobic mesophilic and lactic acid bacteria, aw, pH, acidity, salt levels, and A, B, C, and D enterotoxins were examined at 11 different periods. The level of S. aureus in cheese samples produced from pasteurised milk decreased regularly after 15 d, and on the 90th d was reduced to log 2 cfu/g. In cheese made from raw milk, the S. aureus levels at the beginning of the ripening period increased up to 10⁷ cfu/g, and then decreased to the starting level on the 90th d of the ripening. Throughout the ripening period, enterotoxin A was observed in the curd stage in pasteurised cheese samples inoculated individually with toxin A producing strain. Enterotoxins A, B, C, and D were observed on the third day in pasteurised mixed cheese inoculated with the mixture of 5. aureus strains. Enterotoxin C was observed in cheese inoculated with toxin C producing strain on the 15th d. No enterotoxin was observed in cheese samples made from the raw milk during the processing until the end of the ripening period, and the pH levels did not drop compared to those of pasteurised cheeses.
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