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1

Regulation of calcium channel in the plasma membrane of hepatoma H-35

100%
Niewczas B., Duszynski J.
Biophysics of Membrane Transport
|
1994
|
tom 12
|
nr 2
2

The effect of methotrexate on actin and invasiveness of hepatoma Morris 5123 cells in culture

75%
Otrocka M., Verschueren H., Malicka-Blaszkiewicz M.
Acta Biochimica Polonica
|
2001
|
tom 48
|
nr 4
Monomeric (G), total (T) and filamentous (F) actin and the state of actin polymerisa­tion (F:G) were determined and actin filaments were visualized in hepatoma Morris 5123 cells cultured in the presence of methotrexate (MTX) at various concentration. The exposure of the cells to this drug resulted in a decrease of total and polymerised actin in cytoplasm and in some changes in actin filament organization. This coincided with a decrease of the cells' ability to migrate through Matrigel coated filters and with inhibition of tumour formation after reimplantation of the methotrexate treated cells to experimental rats.
3

Comparison of pyruvate kinase variants from rat liver and Morris hepatoma 7777, obtained by an affinity chromatography on Blue Sepharose CL-6B

75%
Ignacak J., Guminska M.
Acta Biochimica Polonica
|
1993
|
tom 40
|
nr 2
Fractions A (salted out by ammonium sulphate between 21 - 30% saturation), and fractions B (salted out between 51 - 70% saturation) of pyruvate kinase (EC 2.7.1.40.) corresponding respectively to pyruvate kinase types L and M2 from rat liver and Morris hepatoma 7777 were purified by an affinity chromatography on Blue Sepharose CL-6B. Peaks of inactive proteins were eliminated and the enzyme fractions bound biospecifically to the gels were eluted by free ADP. The molecular mass of purified hepatoma pyruvate kinase fraction B was smaller than that of liver pyruvate kinase fraction B. Morris hepatoma pyruvate kinase fraction B represented a variant of type M2, characterised by greatest affinity to 2-phosphoenolpyruvate as a main substrate and different sensitivity to low-molecular effectors in comparison with types L from both liver and hepatoma and in comparison with type M2 from normal rat liver. Only this hepatoma fraction B showed a tumour specific sensitivity to L-cysteine and was insensitive to normal signal molecules i.e. to ATP and fructose-l,6-diphosphate which influence liver pyruvate kinase activity. L-Cysteine inhibited the tumour fraction B of pyruvate kinase by decreasing its Vmax and increasing the Km values in relation to 2-phosphoenolpyruvate.
4
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Content available

Transcription factors as targets of the anti-inflammatory treatment. A cell culture study with extracts from some Mediterranean diet plants

63%
Stalinska K., Guzdek A., Rokicki M., Koj A.
Journal of Physiology and Pharmacology. Supplement
|
2005
|
tom 56
|
nr 1
157-169
During the inflammatory response at least 2 transcription factors, NF-kB and AP-1, are involved in the altered profile of gene expression. We used human hepatoma (HepG2) and human umbilical vein endothelial cells (HUVEC) as a model system: NF-kB and AP-1 were activated by the proinflammatory cytokine IL-1 in the absence or presence of 21 selected plant extracts and the effect was evaluated by the electrophoretic mobility shift assay (EMSA). In both types of cells activation of NFkB by IL-1 was significantly inhibited by extracts from Scandix australis and Artemisia alba, whereas extracts from Amaranthus sp., Eryngium campestre, Thymus pulegioides and Reichardia picroides elicited cell-type dependent response. The IL-1-induced AP-1 activation was diminished by extracts from Scandix australis, Amaranthus sp. and Artemisia alba more potently in HUVEC, while extracts from Urospermum picroides and Scandix pecten-veneris in HepG2 cells. Inhibitory activities of plant extracts towards cytokine activated NF-kB and AP-1 depend to some extent on the order of addition of IL-1 and plant extract to the cell culture, but the mechanism of action of extract components is not clear: although plant polyphenols may participate they are unlikely to be the only mediators, and MAP kinases were found generally not involved in down-regulation of transcription factors activity by plant extracts.
5

Cytotoxicity studies of lasalocid and silibinin in rat hepatoma cell culture

63%
Radko L., Cybulski W., Rzeski W.
Bulletin of the Veterinary Institute in Puławy
|
2011
|
tom 55
|
nr 3
The cvtoprotective effect of silibinin in course of cytotoxicity induced by lasalocid had been measured in rat hepatoma FaO cell line. In the course of the study, MTT test (cellular metabolism), coomassie brillant blue binding test - CBB (total cellular proteins), and LDH release test (membrane integrity) were applied. In addition, changes in the cell morphology after 24 h treatment were observed by light microscopy. The effective concentrations, EC₅₀ were quantified for each compound alone, whereas the nature of their co-action was assessed by isobologram plotting. Lasalocid EC₅₀ ranged from 4 to 10 µM and microphotographs showed significant morphological changes of the cells after 24 h exposure. Silibinin EC₅₀ ranged from 40 to 42 µM for MTT and CBB assays, and 63 µM for LDH assay, and no significant morphological changes occurred. When lasalocid EC₅₀ was used in combination with silibinin in 1-250 µM concentrations, the EC₅₀ values were plotted at 36 µM and 72 µM in MTT and LDH assays, respectively. Thus co-actions of the two drugs led to significant diminishing of lasalocid cytotoxicity in respect to cellular metabolism and membrane integrity. The isobolograms showed remarkable antagonistic effect of silibinin in the course of lasalocid cytotoxic action. Although a considerable interaction was concisely relevant to hepatoma cell line FaO, the promising results incline to extend the study on other in vitro models (primary hepatocytes), as well as to investigate in vivo the cytoprotective effect of silibinin against lasalocid in target animals.
6

Differential effect of interleukin-1 on interleukin-6 stimulated alpha-1-antichymotrypsin expression in human hepatoma cell lines

63%
Rokita H., Bartle L., Sipe J. D.
Folia Histochemica et Cytobiologica
|
1992
|
tom 30
|
nr 4
7

Ultrastructural evaluation of Morris hepatoma metastases to the lungs after local administration of Mutein VI hrecTNF-alpha

63%
Nowak H. F., Terlikowski S., Poludniewski G., Sulkowski S., Szymanska B.
Folia Histochemica et Cytobiologica. Supplement
|
1996
|
tom 34
|
nr 1
8

The effect of experimental neoplastic disease on malondialdehyde level and glutathione status in erythrocytes of rats

63%
Batko J.
Acta Biochimica Polonica
|
1997
|
tom 44
|
nr 4
The level of lipid peroxidation products and the content of glutathione in erythrocytes of rats with Morris 5123 hepatoma at different stages of tumor development were examined. The content of endogenous malondialdehyde (MDA) was increased throughout all periods of tumor development as compared to the results for healthy rats. From the extent of MDA generation under oxidative stress we concluded that erythrocytes of Morris 5123 hepatoma bearing rats were more susceptible to autoxidation than those from control rats. The content of reduced glutathione (GSH) and oxidized glutathione (GSSG) was increased at the early stage of tumor growth. At the advanced stage of the disease both the content of GSH and the GSH/GSSG ratio were decreased while the content of GSSG remained at the elevated level.
9

Arachidonic acid-induced apoptosis in rat hepatoma AS-30D cells is mediated by reactive oxygen species

51%
Dymkowska D., Wojtczak L.
Acta Biochimica Polonica
|
2009
|
tom 56
|
nr 4
711-715
 Arachidonic acid at micromolar concentrations produced a drastic increase of the generation of reactive oxygen species (ROS) in rat hepatoma AS-30D cells cultivated in vitro along with an increase in the incidence of apoptotic cell death. Both processes were prevented by trolox, a water-soluble tocopherol derivative, and tempol, a known antioxidative agent. A synthetic hybrid of lipoic acid and trolox or preincubation with N-acetylcysteine were less effective. Preincubation of the cells with etomoxir, a known highly specific irreversible inhibitor of carnitine-palmitoyltransferase I, partly decreased the ROS formation induced by arachidonic acid but it did not affect the increase in apoptosis. Cumulatively, these results indicate that apoptosis induced in hepatoma cells by arachidonic acid is mediated by ROS. They also suggest that this effect is due to arachidonic acid as such and not to its mitochondrial oxidative metabolites.
10
Content available remote

Actin cytoskeleton and beta-actin expression in correlation with higher invasiveness of selected hepatoma Morris 5123 cells

51%
Popow A., Nowak D., Malicka-Blaszkiewicz M.
Journal of Physiology and Pharmacology. Supplement
|
2006
|
tom 57
|
nr 7
111-123
Our studies were focused on the isolation and characterization of highly motile fraction of cells from hepatoma Morris 5123 population. Cells that underwent several migration cycles through Matrigel - coated filters were successfully cultured. The invasion index was determined by means of Matrigel invasion assay. Statically significant increase in the value of invasion factor for selected cells variant in comparison to the parental population was observed. The considerable changes in the cell shape were followed by the reorganization of the actin cytoskeleton structure including a dense subcortical congestion in the distribution of ß-actin isoform. The visualization of this protein in tumor cells was performed by immunostaining and scanning fluorescent confocal microscopy. The results were confirmed by densitometry analysis of Western blots. In addition, the increased state of actin polymerization in the cytoplasmic fraction of selected cells was determined as measured by filamentous to monomeric (F:G) actin ratio. Concluding, the selected fraction of hepatoma Morris 5123 cells with higher invasion capacity was characterized by rounded shape, remarkable increase of ß-actin level, its submembrane concentration as well as with the increased state of actin polymerization with respect to parental cells population.
11

Local reaction from eosinophils in the development of Morris hepatoma after hrecTNF-alpha administration

51%
Terlikowski S., Nowak H. F., Poludniewski G.
Folia Histochemica et Cytobiologica. Supplement
|
1996
|
tom 34
|
nr 1
12
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Differential responses of hematopoietic and non-hematopoietic cells to anti-inflammatory cytokines: IL-4, IL-13 and IL-10

51%
Guzdek A., Stalinska K., Guzik K., Koj A.
Journal of Physiology and Pharmacology
|
2000
|
tom 51
|
nr 3
13

N-acetylneuraminic acid, phosphate and thiol groups of pyruvate kinase isoenzymes from Morris hepatoma 7777 and normal rat liver

51%
Ignacak J., Guminska M.
Acta Biochimica Polonica
|
1997
|
tom 44
|
nr 2
The highest amount of N-acetylneuraminic acid (AcNeu) was found in pyruvate kinase isoenzyme L from normal rat liver (24 moles/mole of enzyme tetramer), with the highest electrophoretic mobility. On the other hand, isoenzyme M2 from Morris hepatoma 7777, with the lowest electrophoretic mobility, had the lowest AcNeu content (5 moles/mole of enzyme tetramer). This tumour isoenzyme M2 of pyruvate kinase was, however, characterised by the highest phosphate content (12 moles/mole protein), in comparison to isoenzyme L (3 moles/mole protein) or normal liver isoenzyme M2 (6 moles/mole protein). This could indicate a regulatory change caused by reversible enzyme phosphorylation and dephosphorylation or sialization and desialization. Despite these differences, the sum of the two negatively charged residues was lower in tumour pyruvate kinase isoenzyme M2, with the slowest migration rate, than in normal rat liver isoenzyme M2. Moreover, isoenzyme M2 from tumour material, in comparison with isoenzyme M2 from normal rat liver, had a twice as high content of thiol groups (20 moles/mole protein), especially of free and superficially located ones, than the isoenzyme M2 from normal liver (10 moles/mole protein). This may explain abnormal susceptibility of tumour isoenzyme M2 to stereospecific inhibition by exogenous L-cysteine, and indicate genetically dependent changes in amino-acid content of tumour enzyme which take place during cell tumourigenic transformation.
14

Complex analysis of genes involved in the inflammatory response: interleukin-1-induced differential transcriptome of cultured human hepatoma HepG2 cells

51%
Koj A., Jura J.
Acta Biochimica Polonica
|
2003
|
tom 50
|
nr 3
The systemic inflammatory reaction (acute phase response) is induced by many nox­ious stimuli but in all cases the inflammatory cytokines, such as interleukin-1-beta (IL-1/ß) and interleukin-6 (IL-6), are involved. Liver cell response to inflammation manifested by a characteristic change in the profile of synthesized plasma proteins (acute phase proteins) has been extensively studied. Here we describe a model system of cultured human hepatoma HepG2 cells stimulated with IL-1/ ß to evaluate the trans­criptome induced by this cytokine during 24 h of treatment. By using differential dis­play analysis we found IL-1/ß-induced upregulation of several genes coding for cellular trafficking/motor proteins, proteins participating in the translation machinery or in­volved in posttranscription/posttranslation modifications, proteases, proteins in­volved in cellular metabolism, activity modulators, proteins of the cell cycle machin­ery and also some new proteins so far functionally not classified.
15

Organizacja genetyczna wirusa zapalenia watroby typu B

38%
Sidorkiewicz M., Plucienniczak A.
Postępy Biochemii
|
1993
|
tom 39
|
nr 2
99-104
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