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1

Effect of bis-ammonium quaternary salts on the red blood cells

100%
Kleszczynska H., Kilian M., Przestalski S., Witek S.
Postępy Biologii Komórki
|
1989
|
tom 16
|
nr 1
2

Hemolytic activity of new linear surfactin analogs

100%
Dufour S., Deleu M., Wathelet B., Thonart P., Nott K., Paquot M.
Cellular and Molecular Biology Letters
|
2005
|
tom 10
|
nr Suppl.
3

Hemolytic activity of fractions of the ethanolic extract from faba bean cotyledons

75%
Karamac M., Kosinska A., Shimoyamada M., Amarowicz R.
Polish Journal of Natural Sciences. Supplement
|
2006
|
tom 03
79-85
4

Determination and haemolytic activity of saponins in hairy root culture of Platycodon grandiflorum A.DC.

75%
Urbanska N., Nartowska J., Skorupska A., Ruszkowski D., Giebultowicz J., Olszowska O.
Herba Polonica
|
2009
|
tom 55
|
nr 3
103-108
The sum of saponins in the hairy root lines (6, 17) of Platycodon grandiforum A.DC. was compared. Hairy root line 6 showed a higher total saponin content (6.92%) than the line 17 (6.01%). According to the Chinese Pharmacopoeia standards the content of saponins in Platycodi Radix should be not less than 2%. Our results seem to indicate that the hairy root culture of Platycodon grandiforum A.DC. is a good source of saponins. The Haemolytic Index of the hairy root line 6 was 1600. Digitonin was used as a reference. Moreover, the haemolytic activity of TLC subfractions of saponins varied.
5
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Content available

Haemolytic and proteolytic activity of coagulase-negative staphylococci isolated from mastitis cows

75%
Bochniarz M., Wawron W.
Polish Journal of Veterinary Sciences
|
2012
|
tom 15
|
nr 1
The aim of the present study was to assess the haemolytic and proteolytic activity of coagulase- negative staphylococci (CNS) isolated from cows with mastitis. The study was conducted on 100 CNS strains: S. xylosus (n=28), S.chromogenes (n=26), S.haemolyticus (n=25), S. sciuri (n=14), S. warneri (n=4), S.hominis (n=2), S.saprophyticus (n=1); 22 CNS were isolated from cows with clinical mastitis and 78 from those with subclinical mastitis. The CNS studied showed the ability to produce only α-haemolysin and belonged to one strain – S. haemolyticus (21.0% of isolated CNS strains). Haemolysin-positive CNS were responsible for both clinical and subclinical mastitis (22.7% and 20.5%, respectively). The ability to produce protease was found in 31.0% of CNS belonging to two strains: S. chromogenes and S. sciuri. Protease-positive CNS were the etiological factor of both clinical and subclinical mastitis (31.8% and 30.8%, respectively). All S. xylosus, S. warneri, S. hominis, and S. saprophyticus strains were found protease-negative and haemolysin-negative, irrespective of whether they caused clinical or subclinical mastitis in cows.
6

The physicochemical properties of some new aminophosphonates

75%
Kleszczynska H., Sarapuk J., Dziamska A.
Cellular and Molecular Biology Letters
|
2000
|
tom 05
|
nr 4
415-422
The hemolytic activity of some newly synthesized aminomethanephosphonic acid derivatives was studied. The compounds studied differed in their polarity and the hydrophobicity of the electronic substituents at their nitrogen and carbon atoms. It was found that acyclic aminophosphonates exhibited significantly stronger hemolytic properties than cyclic aminophosphonates. To cause the same level of hemolysis of pig erythrocytes, it was necessary to use about a tenfold higher concentration of cyclic aminophosphonates than acyclic ones. The hemolytic activities of the compounds were related to the possibilities of their incorporation into the lipid phase of erythrocyte membranes. Once incorporated, they changed the fluidity of those membranes; the changes were more pronounced in the case of cyclic aminophosphonates. Acyclic compounds were also found to exhibit a slight antioxidative activity, which may be a consequence of their stronger interaction with the membrane lipids. The results obtained in the experiments performed with the use of planar lipid membranes were similar to those obtained in the hemolytic studies, i.e., acyclic aminophosphonates interacted more effectively with those membranes. The general conclusion is that both stereochemistry and hydrophobicity are the factors that determine the efficiency of the interaction of the compounds studied with the model membranes used, and that most likely location of aminophosphonates is the lipid phase of the erythrocyte membrane. Another conclusion is that newly synthesized aminophosphonates may be used as potentially good pesticides.
7
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Content available

The in vitro hemolytic activity of erythrocytes from free-living mute swan (Cygnus olor) spread out in Gdynia and Sopot Coasts

75%
Tkachenko H., Kurhaluk N., Hetmanski T.
Baltic Coastal Zone. Journal of Ecology and Protection of the Coastline
|
2018
|
tom 22
The erythrocytes could be considered as a valuable model in studying the cytotoxic effect of environmental xenobiotics owing to its particular structure and the important components of their plasma membrane especially lipids and proteins. The aim of our study was to investigate the erythrocyte hemolytic activity of mute swan (Cygnus olor) from Baltic coastal region in two points, i.e. Sopot and Gdynia. These comparisons will show whether mute swans on the Baltic coastal regions have elevated levels of hemolysis plus gender variation in erythrocyte damage. Forty adult mute swans were captured from Sopot and Gdynia in February 2013. Collected birds were measured and weighted, and the blood was sampled. The birds were divided into 4 groups (after the 2nd/3rd year of life): males from Sopot (n = 10), females birds from Sopot (n = 10), males from Gdynia (n = 10), and females from Gdynia (n = 10). Blood samples were collected from the medial metatarsal vein in sodium citrate for erythrocyte sedimentation rate. Blood was stored in tubes and held on the ice until centrifugation. The plasma was removed. The erythrocyte suspension (one volume) was washed with five volumes of saline solution three times. The acid resistance of erythrocytes was induced by 0.1M HCl, while osmotic resistance was induced by incubation erythrocyte suspension with the different solution of urea and NaCl, as well as in solutions of different NaCl concentration (0.1-0.9%). The erythrocyte hemolysis was measured spectrophotometrically. Our study revealed that the highest level of the hemolysis percentage was observed among females from Gdynia, males from Sopot, then females from Sopot, and males from Gdynia. The significant differences between the hemolysis percentage among females from Gdynia and Sopot from 13.5 to 18.0 min after HCl-induced hemolysis was observed. The results also indicated significant differences between the hemolysis percentage among males from Gdynia and Sopot from 14.0 to 19.0 min. Hemolysis percentage showed to be increased not significantly among males from Gdynia with time from hemolysis initiation to 10.0 min but close to linearity with a value of hemolysis percentage among males from Sopot after 25 min of hemolysis initiation. The maximal level of hemolysis percentage was significantly higher among females from Gdynia (at 15.0 min) compared to females from Sopot (at 14.0 min). The maximal level of hemolysis percentage among males from Gdynia and Sopot was noted at 0.5 min after hemolysis initiation. The maximal level of hemolysis percentage was 4.40% and 4.11%, respectively. The highest percentage of hemolyzed erythrocytes after incubation with solutions of various urea concentration was observed both among males and females from Gdynia. The lowest percentage of hemolyzed erythrocytes was exhibited by the erythrocyte suspension of males from Sopot. Comparing the percentage of hemolyzed erythrocytes both among males and females from Sopot, the higher values of hemolyzed erythrocytes was noted among females. The highest percentage of hemolyzed erythrocytes after incubation with solutions of various NaCl concentration was observed among males and females from Sopot and further among males and females from Gdynia after incubation with 0.5-0.9% NaCl solution. Comparing the percentage of hemolyzed erythrocytes after incubition with 0.1-0.4% NaCl solution, the highest percentage of hemolyzed erythrocytes was observed among males and females from Gdynia and then among males and females from Sopot. Our study showed more individual variation, probably related to the impact of the environmental habitat and consumption of ingested prey items by mute swans. Of those males and females, females from Gdynia showed the highest level of hemolyzed erythrocytes.
8

Changes in erythrocyte membrane permeability induced by verapamil, chlorpromazine, and their combinations with amphotericin B

63%
Knopik-Skrocka A., Bielawski J., Chowanski S., Walkowiak P.
Biological Letters
|
2011
|
tom 48
|
nr 2
9

Gemini ester quat surfactants and their biological activity

63%
Luczynski J., Frackowiak R., Wloch A., Kleszczynska H., Witek S.
Cellular and Molecular Biology Letters
|
2013
|
tom 18
|
nr 1
Cationic gemini surfactants are an important class of surface-active compounds that exhibit much higher surface activity than their monomeric counterparts. This type of compound architecture lends itself to the compound being easily adsorbed at interfaces and interacting with the cellular membranes of microorganisms. Conventional cationic surfactants have high chemical stability but poor chemical and biological degradability. One of the main approaches to the design of readily biodegradable and environmentally friendly surfactants involves inserting a bond with limited stability into the surfactant molecule to give a cleavable surfactant. The best-known example of such a compound is the family of ester quats, which are cationic surfactants with a labile ester bond inserted into the molecule. As part of this study, a series of gemini ester quat surfactants were synthesized and assayed for their biological activity. Their hemolytic activity and changes in the fluidity and packing order of the lipid polar heads were used as the measures of their biological activity. A clear correlation between the hemolytic activity of the tested compounds and their alkyl chain length was established. It was found that the compounds with a long hydrocarbon chain showed higher activity. Moreover, the compounds with greater spacing between their alkyl chains were more active. This proves that they incorporate more easily into the lipid bilayer of the erythrocyte membrane and affect its properties to a greater extent. A better understanding of the process of cell lysis by surfactants and of their biological activity may assist in developing surfactants with enhanced selectivity and in widening their range of application.
10

Haemolytic activity of Mycobacterium spp.

63%
Brzychcy M., Andrzejczyk Z., Zalewska N., Zwolska Z., Rudnicka W.
Acta Microbiologica Polonica
|
1997
|
tom 46
|
nr 4
11

Hemolysis and antioxidative protection of erythrocytes by functionalized quaternary ammonium salts

63%
Kleszczynska H., Bonarska D., Oswiecimska M., Sarapuk J.
Polish Journal of Environmental Studies
|
2003
|
tom 12
|
nr 1
The results of studies on hemolytic and antioxidative activities of quaternary ammonium bromides and chlorides differing in alkyl chain length and with functionalized antioxidative group are presented. Pig erythrocytes (RBC) and their ghosts were used in experiments. The hemolytic studies permitted to determine the safe concentrations at which the compounds studied did not damage RBC membranes. RBC were then UV-irradiated and the antioxidative efficiency of the quats determined. It was found that hemolytic activities increased with lipophilicity of a compound and each bromide salt was more hemolytic than chloride ones. The antioxidant activity of the quats followed the same rule. The results obtained indicate that observed effects are the consequence of the incorporation of particular compounds to different depths into the lipid phase of the RBC membrane. The incorporation depended on lipophilicity of quats and the kind of counterions. Studies on fluidity changes induced by quats in ghost membranes confirmed the above conclusion.
12

Comparison of acute haematotoxicity of 2-methoxyethanol, 2-ethoxyethanol and 2-butoxyethanol in male rats

63%
Starek A., Szabla J., Szymczak W., Zapor L.
Acta Toxicologica
|
2006
|
tom 14
|
nr 1-2
13

Assessment of biological activity of new compounds designated to act as lysosomotropes

63%
Kleszczynska H., Bonarska D., Luczynski J., Witek S., Sarapuk J.
Polish Journal of Environmental Studies
|
2005
|
tom 14
|
nr 6
This work contains the results of studies on the influence of new lysosomotropic substances on an erythrocyte membrane. The compounds studied were hydrochlorides of N,N-dimethylglycine alkyl esters (DMG-n) and N,N-dimethylalanine alkyl esters (DMAL-n) having two different-length alkyl chains (n = 12 and 16), oxalates of dimethylaminoalaninates (DMALs -n; n = 8, 10, 12, 14 and 16) and methobromides of glycinates and alaninates (DMALM-12 and DMGM-12). They were found to hemolyze erythrocytes, to change their osmotic resistance and to influence erythrocyte membrane fluidity. The results obtained indicate that observed changes were dependent on lipophilicities of the compounds. It was especially evident in the case of hemolytic efficiencies of the homologous series of alanine oxalates. Also, DMG-n and DMAL-n compounds significantly differed in their hemolytic properties. Again, slightly better hemolytic efficiency of DMG compounds in comparison with corresponding compounds having the same alkyl chain, DMAL, confirm such a conclusion. However, their hemolytic efficiencies were found to be moderate, which makes them potentially useful membrane modifiers. That feature is important for lysosomotropic compounds and its confirmation was the primary aim of the presented work. It is worth mentioning that DMGM and DMALM compounds exhibited better hemolytic efficiencies than all other compounds studied – which is probably caused by the fact that they were used as bromides. Bromides are commonly found to be more active than compounds with other counterions.
14

Modifications of hemolytic activity of polyene antibiotics by mono- and disaccharides in mammalian erythrocytes

51%
Knopik-Skrocka A., Bielawski J., Wrzeszcz K., Bochanysz A., Nowak K.
Biological Letters
|
2007
|
tom 44
|
nr 1
17-30
15
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

The link between shrimp farm runoff and blooms of toxic Heterosigma akashiwo in Red Sea coastal waters

51%
Mohamed Z. A., Al-Shehri A. M.
Oceanologia
|
2012
|
tom 54
|
nr 2
In May 2010 a copious bloom of the raphidophyte Heterosigma akashiwo was observed for the first time in Red Sea waters off the coasts of Saudi Arabia. This bloom was confined to an area where water and phytoplankton flow freely between the sea and a shrimp farm. The phytoplankton density and physico-chemical characteristics of the sea water were therefore investigated weekly at bloom and non-bloom sites in order to gain insight into the environmental factors prevailing at the bloom site and their link with the shrimp farm runoff. The bloom site showed higher nutrient concentrations than the non-bloom site, indicating the possible role of the shrimp farm in flushing nutrients into this site. The bloom appeared on 27 May, coinciding with a decrease in salinity (<300/00) and an increase in temperature (>19◦C). The results of toxicological assays showed that both bloom samples and batch cultures of H. akashiwo were toxic to Artemia salina and exhibited haemolytic activity with respect to rabbit erythrocytes. Bloom samples showed a higher toxicity (LC50 = 8.9 × 104 cells ml−1) and haemolytic activity (EC50 = 3.64 × 104 cells ml−1) than the batch cultures (LC50 =11.6×104 cells ml−1, EC50 =5.1×104 cells ml−1). In the light of the results of this study, the link between H. akashiwo blooms and shrimp farm runoff should be considered during the monitoring of Red Sea coastal waters for the presence of harmful algal blooms.
16

Association of listeriolysin O with the cell surface of Listeria monocytogenes

51%
Bielecki J.
Acta Microbiologica Polonica
|
1994
|
tom 43
|
nr 3-4
17

Insertions within iap gene of Listeria monocytogenes generated by plamid pLIV are not lethal

51%
Bielecki J.
Acta Microbiologica Polonica
|
1994
|
tom 43
|
nr 2
18

Hemolytic activity of the non-ionic detergents Tween 80 and Triton X-100

38%
Bielawski J., Mrowczynska L., Konarczak M.
Biological Bulletin of Poznań
|
1995
|
tom 32
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