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The objective of the present investigation was to evaluate the effect of nanodiamond (ND) particles on rats health status. 1 mg/kg b.w. of nanodiamond particles was administrated intravenously and intraperitoneum. The presence of an adverse impact was examined. The results show significant changes in biochemical (glucose and total protein level decrease) and hematological (elevated platelets count) parameters, only in case of intravenous injection.
This study aimed to determine the relationship between hepcidine levels and some hematological and biochemical parameters after anemia in natural theileriosis cows. This research was conducted with a total of 25 cattle, including 10 healthy controls and 15 cattle with natural theileriosis. 1098 bp positivity was determined according to the PCR result. Leukocyte (WBC), erythrocyte (RBC), hematocrit (HCT) and hemoglobin (HGB) levels were decreased. However, red blood cell distribution width (RDW), Platelet (PLT) and hepcidin levels were increase determined in natural theileriosis cows according to the control group. In addition, a positive correlation was detected between hepsin and iron parameters although there is no statistical significance between them and a negative correlation was determined between hepcidine and HCT, HGB and RDW parameters. As a result, it was determined that erythrocyte parameters decrease and increase in iron parameters and heptidine levels in cattle with theileriosis. It can be concluded that the diagnostic importance.
Monitoring the health of dairy herds is central to the assessment of animal health and welfare. The aim of this study was to analyse hematological and biochemical parameters in Holstein-Friesian dairy cows depending on the management system.Thirty cows from the foundation stock with an average annual yield of 6500-7000 l of milk were selected for the experiment. The cows were characterized by similar yields and were in a similar lactation period (second and third lactation). The cows were divided into three groups of 10 cows each: confined to a barn (control group C), housed in a barn with free access to an outdoor run (O) and maintained on a pasture (P). Blood samples to determine hematological and biochemical parameters were collected from animals on an empty stomach before the onset of the experiment and towards the end of the pasture period. The present study showed a positive effect of pasturing dairy cows on their hematological parameters. In the group of pasture-fed cows, there were also significant (P≤0.05) differences between the results before and at the end of the trial. There was a significant(P≤0.05) increase in total white blood cell count and in basophil and neutrophil percentages. There were also significant (P≤0.05) increases in the concentration of hemoglobin (by 8%) and hematocrit (by 7%). Biochemical analysis showed slightly higher urea concentrations in the group of pastured cows when compared to the other groups. The high content of nitrogen compounds in the pasture forage could have a certain effect on hepatic nitrogen metabolism, which could increase serum concentrations of AST and ALT enzymes in cows from group P.
Permanent changes in the surrounding environment cause long-term stress in birds, which, when lasting days or weeks, affects the activity of the immune system and increases susceptibility to diseases, leading to changes in the levels of haematological parameters. The heterophil-to-lymphocyte ratio (H:L-ratio) is generally considered an independent and robust indicator of stress level in birds. This parameter allows in a simple way to evaluate activity of the immune system and individual health state of adult and nestling birds. It also enables assessing a body response to short- and long-term stress induced by, among others, the surrounding environment, social stress, blood parasites or a greater energy expenditure of females during breeding. Under conditions of field work the determination of the H:L- ratio is not difficult because what is only needed to conduct a blood smear test is a drop of blood that can be easily obtained even from birds of a small body mass. Moreover, an increase in the H:L-ratio is observed after about an hour from the moment of catching a bird contrary to other measurements like the determination of a baseline level of corticosterone. In this article available literature that discusses the impact of various factors on the H:L-ratio in the Great Tit as a species of 'fast-paced' life is reviewed. In adult and nestling birds the H:L-ratio is influenced by various factors — ecological and ecophysiological ones. In some cases the same factor, e.g. brood size manipulation or a type of habitat, can significantly influence the level of the discussed stress indicator as well as it may not show any impact at all. While interpreting the H:L-ratio one must take into account an impact of various ecological and ecophysiological factors on health state, such as habitat, phase of the annual cycle, differences between brood attempts, sex, age as well as on relations with other indicators of condition e.g. body mass or total blood haemoglobin concentration.
Objective. The present study was carried out to investigate the effects of occupational lead exposure on the hematological and biochemical parameters in occupationally exposed and non exposed inhabitants of Karachi. Material and methods. In 100 lead exposed subjects recruited from automobile workshops, lead battery repair and recycling units located in Karachi and in 100 control subjects the general health status, hematological parameters and exposure markers for lead were measured. Results. Results indicated that the mean values of blood lead level and δ-aminolevulinic acid were significantly higher (P<0.05) while the activity of δ-aminolevulinic acid dehydratase were significantly decreased (P<0.05) among battery repair and recycling workers as compared to controls. The abnormalities in the blood lead level, δ-aminolevulinic acid and δ-aminolevulinic acid dehydratase were more frequent in lead exposed battery repair workers when compared with control subjects. The blood lead levels and δ-aminolevulinic acid were positively correlated while δ-aminolevulinic acid dehydratase was found to be negatively correlated with age, years of exposure and years of employment. Blood lead level was positively correlated with hemoglobin and RBC count while δ-aminolevulinic acid dehydratase was negatively correlated with hemoglobin concentration. The work related symptoms, droopiness, nasal symptoms and muscular pain were more frequent among battery repair workers as compared to control group. The findings of present study confirmed that occupational exposure to lead is associated with deviation in important hematological parameters and biological markers of exposure to lead among lead exposed workers, and also confirms the impact of lead exposure in the development of adverse effects among lead exposed workers. Conclusions. The study provides the data for risk assessment in lead battery repair workers of Karachi and suggests the need for preventive measures for battery repair workers and improvements to reduce occupational lead exposures to protect them from lead toxicity. It is suggested that hematological and physical examinations of lead exposed workers should be carried out periodically to prevent future health hazards.
Viele Zuchtbetriebe wenden das ganze Jahr hindurch die Fütterung der Kühe mit Silage zu, die manchmal, vor allem im Frühjahr, von schlechter Qualitet ist. Oftmals berücksichtig man nicht den physiologischen Zustand der Tiere, indem man ihnen groβe Silagengabe sogar in der Hochträchtigszeit verabreicht. Das verurscht bei den Kühen das Auftreten von metabolischen Störungen z.B. Acidosis [1, 5, 6, 10]. Diese Störungen werden dann auf das Fötus übertragen, da die Plazenta in diesem Fall nicht genügend Schutz leistet. Die Kälber, die von solchen Müttern stammen, kommen zur Welt schwach mit einem Durchfall und verenden oft [2, 7, 9]. Die Autoren der Vorgelegten Arbeit versuchten die bei den Kälbern vorkommenden Störungen durch das Verabreichen von einen Braunkohlenextrakt zu korrigieren, in dem sie teure und nicht immer sichere pharmakologische Präparate ersetzen wollten.
The comparative study was performed on 200 European brown hares. Group I consisted of 35 clinically healthy hares kept in cages. Group II comprised 165 clinically healthy hares caught alive in their natural environment. Following the premedication, arterial and venous blood was drawn from the hares of the two groups studied. In the arterial blood, the acid-base balance (ABB) parameters were determined - blood pH, pCO₂, t CO₂, HCO₃⁻, BB, and BE. In the venous blood, the activity of AST, ALT, and FA, concentration of urea, creatinine, total protein, albumins, globulins, cholesterol, triglycerides, glucose, WBC, RBC, Hb, and Ht were determined. In addition, concentrations of Ca²⁺, P, Mg²⁺, Na⁺, K⁺, and Cl⁻ were measured in the blood serum. It was demonstrated that only the contents of globulins and triglycerides were similar in groups I and II. Concentration of electrolytes and ABB parameters were close to each other in the two studied groups, except for concentration of Mg²⁺ and inorganic P, and CO₂ molecular pressure. In the light of the obtained results a question remains opened: whether successful breeding, understood as an increase in the number of offspring of the reintroduced individuals, is more likely to occur in the case of animals caught alive and, adapted to living in their natural environment, or in the case of caged animals.
In gilts, the period of early pregnancy occurring from the time of fertilization to the beginning of implantation is sensitive to any environmental disruptions, including an unbalanced diet of a future mother. Previously, we found that due to the undernutrition in gilts during this period, the endocrine intrauterine microenvironment and DNA methylation in the uterus have been changed. These distortions may diminish the success of pregnancy. In this study we focused on the influence of a restricted diet used in gilts during the first days of pregnancy on their biochemical and haematological parameters in peripheral blood. The applied restrictive diet vs. normal diet covered only 70% of the nutritional demands of early pregnant gilts. Normal (n = 4 gilts) or restrictive (n = 5 gilts) diets were used from the day of the first signs of the estrus until day 9 of pregnancy and biochemical and haematological parameters in blood plasma were determined during peri-implantation period, e.g. on days 15 to16 of pregnancy. In restrictive vs. normal fed gilts significantly lower plasma phosphorus, calcium and total cholesterol as well as the tendency to increasing concentrations of triglicerydes and asparate aminotranserase were found. Haematological parameters did not differ between the studied gilts. Thus, it seems that the availability of nutritional factors became suboptimal in restrictively fed early pregnant gilts. Even short-lasting undernutrition of females during the peri-conceptional period may cause a disruption of biochemical homeostasis during the peri-implantation period and probably affect the success of pregnancy.
The aim of this study was to determine the effect of exploitation of recreation horses horse in the summer season on the selenium concentration in serum and the values of chosen haematological parameters. Blood to analyse was sampled from horses used in recreation in two horse riding centres located in the Western Pomeranian Province. Stable 1 (8 horses), situated in a seaside resort, with rides being led mostly in a riding arena, less often in the field. Large rotation of riders with different level of riding advancement has been observed, with prevalence of novice riders and horses working on the lunge. Stable 2 (18 horses), situated in a rural area, far away from large cities; in the holiday season, horses work maximum 4 hours a day, with prevalence of rides of weak and moderate intensity, being led in the field, with riders with average and high level of riding advancement and small rotation of riders. Blood samples were drawn twice: in the first days of July (start of the holiday season) and in the last days of August (end of the holiday season). Average selenium concentration in the blood serum of horses in Stable 1 decreased significantly during the holiday season. Mean values of chosen analysed haematological parameters of the horses in both riding centres did not undergo any significant changes during their use in the holiday season. Significant increase in the leukocyte and platelet counts after the holiday season was observed in the blood of horses in Stable 1. The horses of Stable 2 were characterised by significantly higher mean haematocrit value (39.14%) when compared to those in Stable 1. No significant correlations were found between the Se concentrations and chosen haematological parameters. The observations being carried out confirm the occurrence of Se deficiencies in horses in the Western Pomerania. As it appears from the performed study, intensive recreational horse use (5-6 hours of work per day) affects the significant decrease in serum selenium concentration, which suggests that application of appropriate supplementation in the nutrition of intensively working horses is necessary. Insufficient number of studies and ambiguous results concerning the effect of horse use on the WBC and the platelet counts prompt to undertake more broader observations in this respect.
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