The aim of presented paper was to assess suitability of SSR markers developed in wheat in order to evaluate genetic similarity of hexaploid species of Avena genus. Three A. sativa cultivars, 3 A. sterilis and 2 A. fatua accessions were analysed. Out of 55 tested microsatellite primer pairs: wms, wmc and gdm 12 were chosen. Based on microsatellite loci diversity genetic similarity was calculated and cluster analysis of NJ (neighbour joining) method was conducted. A dendrogram thopology indicated that A. fatua clustered closer to A. sativa than did A. sterilis. On the basis of conducted research it can be stated that there is a possibility of utilizing wheat microsatellie markers in the analysis of DNA polymorphism of hexapliod species of the Avena genus.