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The peculiarities of heavy metals accumulation by wild medicinal and fruit plants. The article shows the results of studies of heavy metals accumulation by wild medicinal and fruit plants. Special attention is given to the investigation of roadside phytocenoses. Reliable differences in chemical composition of plants from background and transformed habitats are revealed. The influence of morphological and phenological characteristics of the species on the peculiarities of heavy metals accumulation is also determined.
Chromium is a heavy metal; this element is considered as an environmental hazard. Toxicity effects of chromium on growth and development of plants including inhibition of germination process decrease of growth and biomass of plant. The aim of this research is to study accumulation of Chromium along with nutrients and its effect on the growth of Paddy plant (Oryza sativa L). Thus, paddy seedlings grown in petriplates lined with filter paper undergoing, different treatments of Cr (0, 2.5, 5, 10, 25, 50, 75, 100 and 200 mg/L). After one week seedlings were removed and morpho physiological parameters like root length, shoot length and dry weight of plants and Biochemicals ,accumulation of nutrients along with Cr in roots and shoots were determined. The results indicated that the concentrations more than 100 mg/L chromium cause the reduction of morphophysiology parameters in the treatment plants rather than control plant and Cr addition in the cultures caused enhancement of chromium content in roots and shoots of plant seedlings. Similarly the biochemicals and nutrient accumulation also affected by increasing concentrations of chromium. It was also noted that accumulation of chromium in the roots was much higher than the shoots of the seedlings under treatment.
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A model for estimating the precipitation of Cd-, Pb- and As-sulphide in soils at various pH is presented. pH influences not only the solubility of heavy metal-sulphides but also on the distribution of the S atom among sulphide (S²⁻, SH⁻, H₂S) and sulphate (SO₄²⁻) species by the redox potential of the soil solution. The model, based on the Nerst-equation. considers the equilibrium of protolitic processes and complex ion formation. In order to test the model, a long term field experiment polluted with different levels of toxic heavy metals on Hungarian typical soils was performed. Coarse textured soils were treated with Cd. Pb and As salts at pH = 6.8 and 8 and after 6 months and 2 years the heavy metal precipitation was investigated. The Pb ion precipitated at pH = 8.0 and pH = 6.2 while Cd ion at pH = 8. The As ion did not show precipitation effect in any soil according to the majority of arsenate ions at the actual redox potential. Although the solubility of CdS (pK = 26.1) and PbS (pk = 26.6) is similar, the Cd concentration in the solution was higher than that of Pb. The difference between the two ion concentrations is interpreted by the stronger Pb ion adsorption to organic and inorganic soil constituents. It indicated that the adsorbed and complex ion should be considered for the prediction of precipitation.
Lake Mainit is one of the largest lakes recognized as one of Key Biodiversity Areas (KBAs) in the Philippines with rich fishery resources. However, the lake is at risk from heavy metal contamination due to inputs of industrial, agricultural effluents and small-scale mining activities. The present work evaluated levels of heavy metals namely cadmium, lead, and mercury from key aquatic fauna and sediments from seven strategic sections of the lake in 2018. Muscle samples of all seven fish species assessed were below detections limits (BDL) for tHg and Cd. Trace concentrations of Pb in the muscles were detected in Oreochromis niloticus, Glossogobius giuris, Channa striata and Vivipara angularis but values were within safe ranges. Trace concentrations of Pb in the riverine crab (Sundathelpusa sp) exceeded safe limits. Both Cd and tHg were below detection limits in the three invertebrates assessed. Traces of Pb were detected in S4 (Magtiaco) and S5 (Jaliobong) below standard limits (0.05 ppm) only during the southwest (SW) monsoon but Pb were not detected across all stations during the NE monsoon of 2018. For Cd, however, trace concentrations were detected only during the NE monsoon wherein Cd in S2 (Mayag), S3 (Magpayang), S4 (Magtiaco), S5 (Jaliobong), S6 (Dinarawan) and S7 (Kalinawan) exceeded standard limits for Cd in waters (0.01 ppm). Concentrations of tHg in the water were not detected across the two sampling seasons in all seven tributary stations. In sediments, Pb were all detected during the southwest monsoon with highest Pb concentrations in S6 (Dinarawan) and S7 (Kalinawan) which exceeded safe limits. Trace Cd in sediments were mostly below detectable limits. Concentrations of tHg in sediments exceeded safe limits during the SE monsoon in S4 (Magtiaco) and S7 (Kalinawan) areas. These findings recommended that continuous heavy metal monitoring must be conducted. It is also strongly suggested to evaluate the presence of heavy metals in other aquatic organisms and assess the ecological risk posed by these heavy metals though heavy metal speciation analysis.
The research was held in terms of Zaporozhian Reservoir (Ukraine) from 2014 till 2015 year. It was established that prussian carp (Carassius gibelio Bloch, 1782) and roach (Rutilus rutilus Linnaeus, 1758) underyearlings accumulate essential elements more extensively, especially zinc, comparing to adults ones. The species characteristic of heavy metals accumulation in the carp fish body was observed. The intensity level of erythropoiesis occurrence was higher in young fish of both species of carp fish. The specific features of cytometric characteristics of fish erythrocytes were identified: the relative amount of mature red blood cells prevailed in roach, and the area of mature red blood cells was significantly higher in prussian carp. In addition, in the young roach among immature forms of red blood cells significantly higher percentage of polychromatophil normoblasts was revealed.
The objective of this research was to examine the influence of Cd (0, 10, 20, 40 mg Cd·dm⁻³) and Pb (0, 10, 100, 200 mg Pb·dm⁻³) in growing substrate and mycorrhizal colonization of root system on growth, flowering, Cd and Pb accumulation in scarlet sage shoots. Both Cd and Pb had a negative effect on mycorrhizal colonization of scarlet sage roots. The effect of Cd and Pb on the growth of scarlet sage was negligible. Cd at 40 mg·dm⁻³ lowered the number of inflorescences and caused slight chlorosis of the lowermost leaves. Pb at 200 mg·dm⁻³ caused drying of the lowermost leaves. Both heavy metals accelerated flowering of non-mycorrhizal plants, independently of the concentration in growing media. Cd and Pb contents in scarlet sage shoots increased with the increasing content of these heavy metals in growing substrate in both non-mycorrhizal and mycorrhizal plants. Mycorrhization decreased the growth of scarlet sage and increased the accumulation of Cd and Pb in shoots of plants grown in media strongly polluted with heavy metals.
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Plants accumulating heavy metals in the Sudety Mts

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The Sudeten flora consists of some plants we can recognize as heavy metal accumulators. Between others there are: Thlaspi caerulescens, Arabidopsis halleri, Armeria maritima ssp. halleri s.l. and probably the endemic fern Asplenium onopteris var. silesiaca. The authors present the concentrations of some important heavy metals measured in aboveground plant dry weight. The highest concentration of zinc was 8220 ppm (Thlaspi), nickel - 3100 ppm (Thlaspi), lead - 83 ppm (Armeria), copper - 611 ppm (Arabidopsis) and cadmium - 28 ppm (Thlaspi). The concentrations depend rather on species or population specification than on ore deposit quality. There are no typical hyperaccumulator among plants we have examined, but some signs of hyperaccumulation of nickel, zinc and lead could be observed. There are no typical endemic taxa, only Asplenium onopteris var. silesiaca and Armeria maritima ssp. halleri may be recognized as neoendemic taxa, but still of unclear systematic position. During the study we tried to find out why some Sudeten vascular plants do not develop heavy metals hyperaccumulation and why they are rather latent hyperaccumulators. Finally, we suggest to protect some metallicolous areas in spite they are rather territories with low plant biodiversity.
Pollinator crisis (Kearns et al. 1998) and its possible causes has become a worldwide issue during the last two decades. Although pollution is among the possible causes of the widely observed pollinator loss, it is still poorly investigated and no studies are known, so far to test the effects of heavy metal contamination in bumblebees (Bombidae) – the second most important group of managed pollinators after honey bees (Apis mellifera Linneaus). We have tested heavy metal (Pb, Cd, and Zn) accumulation, species diversity and parasite load (focusing on the common Nosema bombi Fantham and Porter, Microsporidia: Nosematidae) in bumblebees. For this purpose, we have chosen three heavy metal gradients (Guryevsk, Belovo and Olkusz) and two additional control sites (Kouznetskiy Alatau and Gornaya Shoria). All gradients were approximately 20 km long, starting in close proximity (1.3 km or less) of an active zinc or metal smelter, and each consisting of 5 sites located on semi-natural or degraded meadows in various distance from the smelter. On each site min. 50 bumblebees were caught by sweep nets, each individual identified to species level and next, its abdomen homogenized and used for assessment of N. bombi infestation. Heavy metal levels in soil of the tested gradients varied between (Pb: 13.6–814.2 mg kg–1, Cd: 0.14–20.3 mg kg–1, Zn: 67.0–889.3 mg kg–1) Bumblebees accumulated Pb and Cd (Pb: 0.21–3.3 mg kg–1, Cd: 0.002–0.069 mg kg–1) in their bodies. The content of these metals in bumblebee bodies correlated with their content in soil (Pb: P <0.01, Cd: P = 0.002). However, no correlation was found between the Zn contents in bumblebees (Zn: 74.7–81.9 mg kg–1) and the soil. We have also found that the metal contents in soil or in the bodies of bumblebees caused no changes in species diversity or dominance on polluted sites, irrespective of type and the level of contamination. The variation of Shannon diversity (H’), as well as Simpson’s diversity (D) were similar in all studied sites and ranged from 0.543 to 0.81 and from 0.152 to 0.484 respectively. The proportion of infected individuals was generally not higher than 0.29 and did not differ significantly among the studied sites. Incidentally, based on variation in the small subunit ribosomal RNA (SSU-rRNA) gene, we have found a new strain of Nosema bombi in the Kouznetskiy Alatau and Gornaya Shoria (West Siberia, Kemerovo Region) samples. The new small subunit RNA sequence in the new strain of N. bombi was named N. bombi WS2 (West Siberia) SSU rRNA. Based on the obtained results we conclude, that bumblebees can withstand or even successfully deal with heavy metal contamination at certain levels.
Root vegetables have greater risk of metal contamination from compost application to soil than other horticultural crops. Moreover, soil organic amendments pose potential environmental hazards. The objective of the present study was to examine the heavy metal uptake in different tissues (petiole, blade, skin, pulp) of Raphanus sativus exposed to organic amendments doses. The impact of the above materials on heavy metal concentration of the soil and plant development parameters were also evaluated. A pot experiment was established with eight treatments arranged in a randomized complete block design and four replicates. Co-compost of sewage sludge and olive wastes at 100, 200, 300 m3 ha–1, composted olive leaves, olive tree pruning wastes, olive mill pomace and poultry manure at 100, 200 m3 ha–1, commercial liquid organic fertilizer at 50 Kg ha–1 with or without inorganic NPK fertilization and a no fertilizing control, were applied to plants. The results showed that sewage sludge application strongly increased the yield and improved radish size cultivated in silt loam soil. The edible radish part had the lowest Fe, Mn, Cu, Zn, and Cr content, whereas the highest Mn, Cu, Zn, Cr was found in the blade and increased Fe, Ni, Pb were recorded in the skin. Organic treatments gave higher Fe, Mn, Cu, Zn amount in both aerial plant tissues compared to the control soil, while Ni, Pb, Cr of all the radish parts were not affected by treatments. This study suggested that organic amendments application gave low permissible levels of all metal content in radish tissues and increased radish productivity. Therefore, organic materials used herein can be applied for normal plant growth without metal contamination of the plant and the soil.
Four soil samples were collected in areas adjacent (sanitary zone) to the Głogów Copper Plant (Poland). They were mostly acidic (pH 4.55 - 4.71) and the total varied widely in their total copper content from 17.03 to 44.07 cMc/kg. The basic copper fractions were determined according to McLaren and Crawford sequential extraction method. Copper of the solution (intensity) expressed as the equilibrium activity ratio, ARoCu when Cu was neither gained nor lost (that is DCuo = 0) as well as labile Cu (LCu) was related to the solid soil phase (quantity) considered as the buffering capacity (BCCu) of the given soils were calculated. The Q/I parameters were estimated at two ionic strengths: 0.005 and 0.010M CaCl2. It was found that all parameters depended on the ionic strength and higher values were obtained at 0.010M CaCl2. An adverse case occurred for the energy of replacement of calcium by copper (DF). Soil properties primarily influenced the magnitude of the Q/I parameters and secondly the high levels of exchangeable and labile soil copper. The low affinity of these soils for copper estimated by the Gapon selectivity coefficients (KG) supported this assumption.
This study measured levels of cadmium, chromium, copper, nickel, lead, and zinc in fruits of Capsicum annuum L., Phaseolus vulgaris L., and Solanum melongena L. during the 2007 vegetation period, to determine levels of metal pollution in Istanbul Province. Plant and soil samples were collected from six sites in Istanbul (Brook Coast area, inner city, industrial area, suburban, roadside, and rural – control – areas). Unwashed and washed leaf and soil samples were analyzed using inductively coupled plasma optical emission spectrometry. The highest and lowest values were as follows: Cd (0.28-0.89 µg·g⁻¹), Cr (5.33⁻¹4.04 µg·g⁻¹), Cu (1.47-5.19 µg·g⁻¹), Ni (3.06⁻¹3.65 µg·g⁻¹), Pb (29.28-86.20 µg·g⁻¹), and Zn (3.70-5.74 µg·g⁻¹). The unwashed samples were more contaminated than the washed samples. Contamination was higher in the vegetables grown in industrial areas and along roadsides. The overall metal concentration pattern in vegetables was Pb > Cr > Ni > Zn > Cu > Cd.
Grzyby rosnące dziko, które są popularnym i cennym surowcem dla przetwórstwa, jak i bezpośredniego spożycia, mogą zawierać w owocnikach metale ciężkie w dużym stężeniu – czasami w reakcji na zanieczyszczenie nimi gleby. Celem pracy było określenie zawartości metali ciężkich w trzech gatunkach grzybów jadalnych, tj. podgrzybku brunatnym (Xerocomus badius), koźlarzu czerwonym (Leccinum aurantiacum), borowiku szlachetnym (Boletus edulis). Pobrano 10 próbek każdego gatunku grzyba z lasów mieszanych Wysoczyzny Siedleckiej z terenu gminy Wodynie (N 52,1 i E 22,03). Grzyby przygotowano według ogólnych zasad zawartych w normie PN-EN 13804:2003, następnie wysuszono je w temperaturze 30-40ºC i rozdrobniono w młynku laboratoryjnym. Otrzymane susze poddano mineralizacji „suchej” według normy PN-EN 14082:2004 w celu oznaczenia Pb, Cd, Cr, Cu, Zn i Ni – metodą FAAS oraz według normy PN-EN 14546:2005 w celu oznaczenia As – metodą HG AAS. Rtęć oznaczono w suszach grzybowych po mineralizacji na „mokro” według normy PN-EN 13806:2003 – metodą CV AAS. Zawartość badanych metali w suszu grzybowym układa się w następujących szeregach malejących wartości: w podgrzybku brunatnym Zn>Cu>Cr>Ni>Cd>Pb>As>Hg; w koźlarzu czerwonym Zn>Cu>Cr>Ni> Cd>As>Pb>Hg; w borowiku szlachetnym Zn>Cu>Ni>Cr>Cd>Pb>Hg>As. Wyniki badań potwierdziły fakt zróżnicowanej zawartości oznaczanych pierwiastków w zbadanych gatunkach grzybów.
W pracy przedstawiono wyniki badań terenowych i laboratoryjnych wybranych właściwości odpadów powęglowych stosowanych do niwelacji powierzchni w aspekcie ich przyrodniczego zagospodarowania. Uzyskane wyniki wskazują, że odczyn badanych materiałów powęglowych jest aktualnie lekko zasadowy i korzystny dla rozwoju większości roślin stosowanych w rekultywacji biologicznej. Pewnym ograniczeniem dla rozwoju roślin może być podwyższone zasolenie. Zawartość metali ciężkich porównana z obowiązującymi w Polsce aktami prawnymi wskazuje, że badany materiał może być wykorzystywany w rekultywacji. Na postawie wyznaczonych współczynników bioakumulacji (BAF) stwierdzono, że gatunki roślin porastających badane składowisko charakteryzują się niskim stopniem akumulacji metali ciężkich. Uzyskane wyniki nie wskazują na fitotoksyczność badanych odpadów powęglowych i pozwalają na stwierdzenie, że materiał zdeponowany na składowisku może być wykorzystywany jako podłoże dla roślin przy zagospodarowaniu przyrodniczym.
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