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Heart rate turbulence in healthy dogs and dogs with dilated cardiomyopathy

100%

Noszczyk-Nowak A.

Polish Journal of Veterinary Sciences

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2012

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tom 15

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nr 3

Heart rate turbulence (HRT) is modulated by the baroreceptor reflex and it was suggested that it could be used as a measure of autonomic dysfunction. Impaired HRT is of a significant prognostic value in humans after myocardial infarction, suffering from dilated cardiomyopathy and patients with heart failure. So far no studies were performed assessing the importance of HRT in dogs. The aim of this study was to prospectively evaluate the HRT turbulence onset (TO) and the turbulence slope (TS) in healthy dogs and in dogs with DCM and to compare the HRT in dogs with DCM that died during the first 30 days of observation and dogs with DCM that survived the first 30 days after the HRT analysis. The current study was aimed at determining reference value of the TO and TS of HRT in healthy dogs (control group) and dogs with dilated cardiomyopathy (DCM group). The tests were carried out on 30 healthy dogs and 30 dogs with DCM composed of Boxers, Doberman pinschers and Great Danes, of different sexes and body weights from 22 to 72 kg, aged between 1.5 and 11.5 years, submitted to the 24-hour Holter monitoring. HRT parameters were calculated using an HolCard software algorithm. TO is a percentage difference between the heart rate immediately following ventricular premature complex (VPC) and the heart rate immediately preceding VPC. TS corresponds to the steepest slope of the linear regression line for each sequence of five consecutive normal intervals in the local tachogram. The average TO in healthy dogs was determined as -13.55 ± 11.12%, TS was 21.33 ± 9.66 ms/RR. TO in dogs with DCM was determined as - 2.61 ± 2.1% and TS was 6.15 ± 3.86 ms/RR. Parameters of HRT were statistically significantly decreased (p<0.01) in dogs with DCM. HRT TO and TS were statistically significantly decreased in dogs with DCM. Dogs with DCM that survived more than 30 days of observation had HRT statistically significantly decreased in comparison to dogs with DCM that died after the 30’th day of observation. Decreased HRT parameters in dogs with DCM suggest an autonomic neuropathy which principally consists of the withdrawal of the cardiac parasympathetic tone. The more the autonomic neuropathy is advanced the faster the death of the dog with DCM might occur, with no correlation with the level of the heart failure.

2

Physiological values of ECG parameters in Silesian horses

100%

Paslawska U., Michalik K., Janus I., Paslawski R., Zysko D., Noszczyk-Nowak A.

Medycyna Weterynaryjna

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2018

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tom 74

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nr 09

The aim of this study was to establish the physiological ECG values in the Silesian horse and to compare the results with literature data for other breeds of horses. This study was carried out on 31 healthy Silesian horses (15 males and 16 females) aged 2-19 years. The ECG was carried out after an anamnesis and clinical examination and without prior premedication or use of force, both of which could affect the ECG result. The mean heart rate (HR) in the Silesian horses was 39 ± 8.1 beats per minute. In the majority of the leads, P waves were single positive waves. Notched waves were present in 16-51% of the horses, bifid waves were found less frequently, and biphasic P waves were seen least frequently. In the aVR lead, the P wave was most commonly found to be single negative. The PQ interval ranged from 0.18 s in the CV1 lead to 0.29 s in the aVR lead. Most often, the PQ interval lasted 0.24-0.28 s. The QRS duration ranged from 0.079 s in the CV1 lead to 0.099 s in lead III. The QRS duration was between 0.08 and 0.1 s. Generally, the T wave was single positive or negative. It was biphasic only in the precordial leads. Unlike P waves, notched T waves were not found. The ECG parameters in the Silesian horse do not differ from normal ECG values for other horse breeds of similar weight. Specific features of the ECG in the Silesian horse are a frequent occurrence of additional R’ and S’ waves in the Einthoven leads and a relatively long supraventricular conduction time.

3

The reaction of healthy hooves, claws and nails to a 3 percentage solution of hydrogen peroxide

100%

Mordak R., Anthony P. S.

Acta Scientiarum Polonorum. Medicina Veterinaria

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2007

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tom 06

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nr 4

4

Prion protein gene polymorphism in healthy and BSE-affected Slovak cattle

84%

Hresko S., Mojzis M., Tkacikova L.

Journal of Applied Genetics

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2009

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tom 50

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nr 4

371-374

Variation of the PrP gene was examined in healthy and BSE-affected Slovak cattle. According to previous studies, the 23-bp indel polymorphism is supposed to be associated with higher susceptibility to BSE. We investigated 301 samples from healthy cattle of various Slovak breeds and 24 samples obtained from tissues of BSE-affected cattle in Slovakia. We examined the PrP gene for the 23-bp indel polymorphism in the putative promoter region, 12-bp indel polymorphism in the first intron of the PrP gene, variations in number of octapeptide repeat units, and presence of the silent AAC>AAT transition in codon 192 within the protein-coding region of the PrP gene. Altogether we found 23 different genotypes in the group of healthy cattle and only 6 genotypes in the group of BSE-affected cattle. Comparison of homozygotes for the 23-bp insertion and heterozygotes showed significant differences (P < 0.05) in genotype distribution between the examined groups. Thereby the homozygous insertion genotype at the 23-bp indel polymorphism site in the promoter region of the prion protein gene seems to have a protective effect against BSE.

5

Levels of parathyroid hormone, calcium, phosphorus and magnesium in blood sera of healthy and sick cows

84%

Spakauskas V., Klimiene I.

Medycyna Weterynaryjna

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2007

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tom 63

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nr 06

662-666

The blood levels of parathyroid hormone (PTH), calcium, phosphorus and magnesium were examined in 122 cows, which included healthy cows as well as cows with osteomalacia and paresis. Levels of PTH were determined in vitro using IMMULITE analyzer, levels of macroelements - using the automated “Eos-Bravo” analyser with Hospitex reagents. The blood level of PTH ranged from 1.7 to 7.5 pmol/l in healthy cows and from 3.95 to 20.19 pmol/l in sick cows. The highest and most significant increase in blood PTH level (up to 18.31 ± 1.88 pmol/l) was found in cows with parturient paresis. The blood serum levels of PTH in healthy cows changed depending on age and on the physiological status, being significantly lower in cows 2-4-years-old compared with cows 8-years-old and over. The levels of PTH were significantly higher in cows-in-calf and heifers-in-calf compared with cows of the lactation period. Higher levels of PTH were found during the winter than in the summer. The blood level of PTH correlated inversely with the level of calcium in cows with osteomalacia (r = -0.89) and with parturient paresis (r = - 0.49-0.61). The blood serum PTH levels were significantly increased in all groups of cows (p<0.05) on the day of parturition and one day after parturition compared with the measured PTH level 5 days before parturition. Vitamin D (injected 3-5 times) administered during the last days before calving effectively increased the levels of calcium, phosphorus and PTH.

6

Virological examination of conjunctival swabs in clinically healthy horses

84%

Zak A., Siwinska N., Bazanow B.

Medycyna Weterynaryjna

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2018

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tom 74

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nr 09

The aim of the study was to determine the presence of the equine influenza virus (EIV), the equine arteritis virus (EAV), and the equine herpesvirus (EHV) in the conjunctival sac of clinically healthy horses. Fifty horses of both sexes and various breeds and ages were included in the study. Virus isolation was carried out in cell cultures (the Madin-Darby canine kidney, green monkey kidney and rabbit kidney cells) from conjunctival swabs. The hemaglutination assay was carried out in order to identify EI viral amplification. The study revealed no presence of the above listed viruses in the conjunctival sac of clinically healthy horses.

7

Values of selected immune and haematological parameters in healthy rabbits

84%

Tokarz-Deptula B., Deptula W.

Polish Journal of Veterinary Sciences

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2005

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tom 08

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nr 2

8

Copper content in neoplastic and healthy mammary glands in dogs

84%

Skibniewska E. M., Kosla T., Skibniewski M.

Bulletin of the Veterinary Institute in Puławy

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2010

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tom 54

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nr 2

269-272

The aim of the investigation was to confirm the hypothesis put forward in human research that copper is cumulated in the neoplastic tumours of the mammary gland. The research material included the post mortem collected healthy mammary glands of bitches and mammary gland neoplastic tumours obtained during routine surgical treatments. The histopathological examinations revealed that among tumours of epithelial origin, the most frequent were adenocarcinomas, which comprised 64% of all neoplastic lesions. The second group included carcinomas - 26%, whereas 10% were the tumours of mesenchymal origin. The lowest copper content was observed in healthy mammary glands. In the tissues with neoplastic lesions, the content of copper was at a much higher level. Statistical analysis revealed a significant difference (P≤0.05) in the copper content between the investigated groups. The performed analyses showed that mammary gland neoplasms cumulate copper ions, and a much higher concentration of this element is observed in the tumours of epithelial origin.

9

Assessment of leukocytes in the bone marrow of healthy Holstein-Fresian cows

84%

Snarska A., Krystkiewicz W., Rekawek W.

Medycyna Weterynaryjna

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2013

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tom 69

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nr 11

Similarly to the production of red blood cells, the production and development of leukocytes takes place in the bone marrow in the process of hematopoiesis. The key stages in the development of leukocytic cell lines are: neutrophilic, eosinophilic and basophilic granulopoiesis, monocytopoiesis, lymphopoiesis and megakaryopoiesis. Leukocytes produced at various developmental stages are characterized by differently shaped nuclei, the presence or absence of nucleoli, and variations in cytoplasm staining. The experiment was carried out on 10 clinically healthy HF cows. Bone marrow aspirate smears stained by the MGG method were analyzed. The study found significant differences in the number, size, and staining intensity of individual myeloid cell lines.

10

Prevalence of the suilysin gene in Streptococcus suis strains isolated from diseased and healthy carrier pigs

84%

Fabisiak M., Kita J., Jedryczko R., Binek M.

Polish Journal of Veterinary Sciences

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2005

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tom 08

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nr 2

11

Regional differences in aorta of goat (Capra hircus)

84%

Ogeng'o J. A., Malek A. K. A., Kiama S. G.

Folia Morphologica

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2010

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tom 69

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nr 4

Regional differences in the aortic wall are important in explaining the physicomechanical properties and disease distribution in this artery. The goat is a suitable model for studying cardiovascular disease, but the regional features of its aorta are scarcely reported. The purpose of the study was therefore to describe the regional differences in the wall of its aorta. Sixteen healthy adult male domestic goats (capra hircus) were euthanised with intravenous sodium pentabarbitone and specimens obtained from the ascending, arch, each vertebral level of descending thoracic, and various segments of abdominal aorta. The specimens were fixed in 10% formaldehyde solution and routinely processed for paraffin embedding. Seven micron thick sections were stained with Mason’s Trichrome and Weigert Resorcin Fuchsin stains. Light microscopic examination revealed that the aortic wall consists of tunica intima comprising endothelium, subendothelial zone and internal elastic lamina, media, and adventitia. Endothelium comprises flat and round endotheliocytes. The population of round cells declines as the internal elastic lamina increases in prominence caudally. Tunica media in ascending, arch, and proximal thoracic aorta comprises two zones: namely a luminal elastic and adventitial musculo-elastic zone, in which muscle islands interrupt some elastic lamellae. These islands progressively diminish caudally until by the eleventh thoracic vertebra they are only patchy. Beyond this point and in the abdominal aorta they are absent and tunica media consists of regular concentric elastic lamellae. Tunica adventitia, on the other hand, increases in thickness and elastic fibre content caudally. Regional variations exist in all three layers of goat aorta. The nature of these differences suggests that they are related to haemodynamic factors. Furthermore, the variations may form the basis for regional differences in physicomechanical strength and disease distribution along the aorta. (Folia Morphol 2010; 69, 4: 253–257)

12

Radiological evaluation of the quadriceps angle (Q angle) in German Shepherd dogs

84%

Lojszczyk-Szczepaniak A., Silmanowicz P., Balicki I.

Medycyna Weterynaryjna

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2014

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tom 70

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nr 01

The research was conducted on a selected group of 65 healthy German Shepherd Dogs to determine Q angle values. The animals were tranquilized, positioned in ventrodorsal recumbency, and examined radiologically. The radiographs were converted into digital form and analyzed by specialized software to determine the lines and to make measurements. The Q angle assessment was based on the determination of two lines according to Kaiser and Miles in previous research. The first line was drawn from the cranial lip of the acetabulum of the hip joint to the centre of the femoral intercondylar notch. The second line was drawn from the femoral intercondylar notch to the tibial tuberosity. The angle between these two lines was the Q angle. The results were statistically analyzed, and basic statistical parameters were calculated, such as statistical mean (Me), standard deviation (SD), as well as minimum (Min) and maximum (Max) values. In our study the mean of the results was 17 with a standard deviation of 7.38. These results are are in agreement with the results of studies carried out on foxes, which suggests that the selection of patients of the same breed, size and similar body weight does not significantly reduce the standard deviation in determining the reference values. The differences between the right and left hind limbs were also observed in our study.

13

Age as a factor influencing diversity of commensal E. coli microflora in pigs

84%

Bok E., Mazurek J., Pusz P., Stosik M., Baldy-Chudzik K.

Polish Journal of Microbiology

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2013

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tom 62

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nr 2

Commensal, intestinal E.coli microflora plays a role in maintenance of intestinal balance of the host, is responsible for defending against pathogenic E.coli. This study encompasses the analysis of BOX-PCR fingerprinting patterns, phylogenetic grouping and virulence genes prevalence among commensal E.coli isolates derived from healthy pigs. Altogether, 274 unique E.coli isolates were identified, 110 from weaned piglets (Piglets I and Piglets II) and 164 from adult sows (Sows I and Sows II). BOX-PCR analysis distinguished isolates from pigs in different age and indicated that during maturation the changes in E.coli microflora occurred. Phylogenetic grouping revealed significant differences between distribution of four phylogenetic groups among isolates derived from piglets and sows. In phylogenetic structure of isolates from the piglets group B1 prevailed significantly, while among isolates derived from the sows the majority of them were classified into phylogenetic group A. The identification of 17 virulence factors in E.coli isolates derived from healthy pigs was performed. Three of 13 intestinal (escV, ehxA, estII) and four extra-intestinal virulence genes (VGs) (hlyA, fimH, papA, sfaS) were detected in the porcine isolates. The percentage of VGs positive isolates among piglets is higher than among sows, moreover, the VGs occurring in E.coli isolates from piglets revealed greater diversity than that detected among isolates from sows.

14

Effect of metabolic disorders accompanying gastroenteritis on the pancreatic exocrine function in piglets. B. Disturbances of the pancreatic exocrine function in piglets with gastroenteritis

84%

Rychlik A.

Polish Journal of Veterinary Sciences

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2002

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tom 05

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nr 3

15

Short-term and long-term PQ, QT and R-R intervals' variability at the resting condition and after exercise testing in healthy Anglo-Arabian horses

84%

Paslawska U., Zysko D., Noszczyk-Nowak A., Paslawski R.

Polish Journal of Veterinary Sciences

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2012

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tom 15

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nr 1

The duration of electrocardiographic (ECG) parameters: PQ, QT and R-R intervals change during long-term and short-term observation as the consequence of the fluctuations in autonomic nervous system activity among others dependent on the exercise and resting. There is no data of horse breed influence on these parameters. The aim of the study was to assess the duration and the variability of the PQ, QT and R-R intervals in the resting conditions and after exercise testing in Anglo-Arabian horses. Material and Methods: 27 healthy Anglo-Arabian horses aged 3.4 ± 1.0 years (15 male, 12 female) had ECG examination in the standing position using Einthoven system of leads. The longest and the shortest PQ, QT and R-R intervals were measured after night rest and after exercise testing and the means were calculated. Conclusions: 1) In Anglo-Arabian horses the difference between the longest and the shortest PQ interval at rest vs. after exercise is 0.06 ± 0.05 vs. 0.03 ± 0.02, QT interval is 0.04 ± 0.03 vs. 0.04 ± 0.04, R-R interval 0.19 ± 0.15 vs. 0.08 ± 0.11. 2) The PQ and R-R intervals reveal high short-term variability either at the resting conditions or after exercise testing. 3) After exercise testing PQ, QT and R-R intervals are shorter than at the resting conditions. The delta PQ and R-R are 2 times smaller in contrast to delta QT which is constant. 4) The PQ and R-R interval variability was greater at the baseline condition than after exercise testing. The QT variability was similar at baseline condition to that after exercise testing.

16

The evaluation of selected parameters of cellular nonspecific immunity in normal and allergic horses

84%

Wilkolek P., Szczepanik M., Golynski M., Adamek L., Pomorska A., Maj-Martyniuk M., Sitkowski W.

Polish Journal of Veterinary Sciences

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2011

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tom 14

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nr 2

The main aim of this study was to compare selected nonspecific immunity parameters in 14 allergic and 12 healthy horses. Each animal was assessed according to the following parameters: in vitro functional capacity of phagocytic cells using the nitro blue tetrazolium chloride reduction test, both spontaneous (NBT) and zymozan stimulated (NBTs), and ingestion capacity of phagocytic cells using a phagocytic index test (IF) and percentage of phagocytosing neutrophils activity (%KF). Differences were demonstrated between the group of allergic horses, especially with severe allergy symptoms, and healthy horses in NBTs values, with higher values in healthy horses. The values of the phagocytic index were significantly higher in horses with allergy.

17

The prevalence of Dirofilaria repens in cats, healthy dogs and dogs with concurrent babesiosis in an expansion zone in central Poland

84%

Bajer A., Rodo A., Mierzejewska E. J., Tolkacz K., Welc-Faleciak R.

Annals of Parasitology

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2016

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tom 62

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nr Suppl.

18

Influence of novel intravenous complex solution of Ca, Mg and phosphates on blood biochemical parameters of healthy and paretic cows

67%

Daunoras G., Dabuzinskas S., Matusevicius A., Cernauskas A.

Polish Journal of Veterinary Sciences

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2008

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tom 11

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nr 1

17-23

The article describes the dynamics of changes in blood concentrations of the active substances present in the solution after its infusion to healthy cows in comparison to NaCI solution as well as the response of paretic cows to treatment with the new complex solution. Cows received a dose of 400 ml of Al solution (containing 8.4 g of Ca2+) intravenously. In healthy cows the average calcium concentration in blood serum prior to the test was 2.52 + 0.08 mmol/1 while 15 min. after the infusion the concentration rose to 3.10 ± 0.08 mmol/1 (p < 0.05) and magnesium concentration rose from 0.61 ± 0.05 to 1.39 ± 0.08 mmol/1 (p < 0.05). This experiment showed that elevated concentration of non-organic phosphates persisted 1 hour after infusion (p < 0.05). In the second phase of efficacy evaluation of the novel preparation Al on paretic cows the intravenous injection of 1 ml/kg of body weight of Al solution increased calcium concentration up to almost normal level (p < 0.05). The level of magnesium in serum 1 h after injection was statistically significantly higher by 63% (p < 0.05) and reached the physiologically normal concentration. 1 h after the infusion of test solution the level of phosphate was higher by 13% (p > 0.05). The rise was statistically not significant. Even though Al solution undoubtedly produced an increase in glucose concentration in the blood serum, due to wide dispersion of individual measurements and high standard deviation the increase (p > 0.05) in glucose concentration was found insignificant. Most of the treated paretic cows rose within 1-6 h after infusion of 400 ml of solution Al. No relapses were observed. A combination of different salts of calcium and magnesium, non-organic phosphates and glucose with analeptic substance mixed in one solution (Al solution) administered at a dose of 1 ml/kg of body weight raises concentrations of essential macroelements in blood serum of cattle and promotes improvement of paretic cows condition.

19

Potential of Pasteurella multocida isolated from healthy and diseased cattle and buffaloes in induction of diseases

67%

Shayegh J., Atashpaz S., Salehi T., Hejazi M.

Bulletin of the Veterinary Institute in Puławy

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2010

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tom 54

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nr 3

Twenty-six isolates of Pasteurella multocida were recovered from cattle and buffaloes in Iran. The identification of the isolates was carried out using morphological and biochemical tests. Among eight different biochemical biovars, 17 (65.38%) of them were assigned to biovar 3 and the rest belonged to biovars 2, 4, 5, 6, 7, and 15. Capsular typing of isolates by PCR demonstrated three capsular types (A, B, and D), among which type B was the most common (46.15%) and was found in 10 (38.46%) cattle and in two (7.69%) buffalo samples. A notably high prevalence of Pasteurella filamentous haemagglutinin A (PfhA ) and transferrin binding protein encoding (tbpA) genes among three virulence genes detected by multiplex PCR were found. The high prevalence of these genes owning association of disease status among healthy animals showed high potential of the strains in induction of disease among cattle and buffalo herds. The results of this study imply that the role of environmental and host factors are more important than bacterial virulence factors (PfhA and tbpA) in disease induction.

20

The occurrence and comparative phenotypic characteristics of Staphylococcus spp. from healthy and diseased, household and shelter dogs, based on routine biochemical diagnostic methods

67%

Kasprowicz A., Bialecka A., Bialecka J., Godzisz I., Barabasz W., Jaworska O., Malachowa N., Miedzobrodzki J.

Polish Journal of Microbiology

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2011

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tom 60

|

nr 1

To determine the staphylococcal colonization pattern in healthy and diseased dogs, living in two particular environments, a number of microbiological samples were taken. Overall, twenty dogs, either healthy or with infected skin lesions, were examined. In each case bacterial swabs were collected from the nasal mucosa, ear, perineum, lumbo-sacralis triangle, and from the infection sites if such were present. A total number of 104 isolates representing different staphylococcal species were isolated and identified using routine biochemical methods applied in diagnostic laboratories. Among 17 isolated staphylococcal species, Staphylococcus intermedius was the most common species isolated from both healthy or diseased dogs living either in animal shelter or household environments. The pattern of Staphylococcus sp. colonization differs considerably for animals living in the two tested habitats. In particular, S. aureus MRSA and MSSA isolates were detected only in infected skin lesion samples from animals that dwelled in the animal shelter. As could be expected, S. intermedius was found to be a predominant causative agent in canine skin infections. In our study, we demonstrated that S. intermedius in its carrier-state, inhabits mainly the mucosal membrane of the nasal vestibule. It was also found in the samples taken from the skin, the lumbo-sacralis triangle and perineum, but was rarely isolated from the ears.

Ograniczanie wyników

Heart rate turbulence in healthy dogs and dogs with dilated cardiomyopathy

Physiological values of ECG parameters in Silesian horses

The reaction of healthy hooves, claws and nails to a 3 percentage solution of hydrogen peroxide

Prion protein gene polymorphism in healthy and BSE-affected Slovak cattle

Levels of parathyroid hormone, calcium, phosphorus and magnesium in blood sera of healthy and sick cows

Virological examination of conjunctival swabs in clinically healthy horses

Values of selected immune and haematological parameters in healthy rabbits

Copper content in neoplastic and healthy mammary glands in dogs

Assessment of leukocytes in the bone marrow of healthy Holstein-Fresian cows

Prevalence of the suilysin gene in Streptococcus suis strains isolated from diseased and healthy carrier pigs

Regional differences in aorta of goat (Capra hircus)

Radiological evaluation of the quadriceps angle (Q angle) in German Shepherd dogs

Age as a factor influencing diversity of commensal E. coli microflora in pigs

Effect of metabolic disorders accompanying gastroenteritis on the pancreatic exocrine function in piglets. B. Disturbances of the pancreatic exocrine function in piglets with gastroenteritis

Short-term and long-term PQ, QT and R-R intervals' variability at the resting condition and after exercise testing in healthy Anglo-Arabian horses

The evaluation of selected parameters of cellular nonspecific immunity in normal and allergic horses

The prevalence of Dirofilaria repens in cats, healthy dogs and dogs with concurrent babesiosis in an expansion zone in central Poland

Influence of novel intravenous complex solution of Ca, Mg and phosphates on blood biochemical parameters of healthy and paretic cows

Potential of Pasteurella multocida isolated from healthy and diseased cattle and buffaloes in induction of diseases

The occurrence and comparative phenotypic characteristics of Staphylococcus spp. from healthy and diseased, household and shelter dogs, based on routine biochemical diagnostic methods