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The studies were conducted in the years 1998–2000 on soybean, cv. Polan. The purpose of the studies was to conduct microbiological and mycological analyses of the leaves and pods of soybean. Bacteria and fungi were obtained much more often from the diseased soybean parts as compared to the healthy ones. 778 fungi isolates were obtained as a result of the mycological analysis of the leaves and pods of soybean. Fusarium spp. and Phomopsis sojae were most frequently isolated within pathogenic fungi. Their proportion was 17.5% and 7.8% of all the fungi, respectively. On the other hand, the isolated saprophytic fungi included for example the species from the genera of Acremonium, Cladosporium, Gliocladium, Penicillium and Trichoderma as well as Aureobasidium pullulans and Epicoccum purpurascens. Besides, about a four times lower number of antagonistic microorganisms (Bacillus spp., Pseudomonas spp., Gliocladium spp., Penicillium spp., Trichoderma spp.) was isolated from the infected soybean parts in comparison to the number of microorganisms isolated from the healthy parts.
The aim of presented experiments was to determine the usefulness of plant extracts for dressing of cereal seeds. Water extracts (macerations, infusions) prepared from different morphological parts of 39 plant species were used as dressing preparations. Non-disinfected seeds of two cultivars of barley (Hordeum vulgare L.): Rudzik (brewing barley) and Stratus (common barley) were used for the purpose of dressing. The experiment was conducted as a filter paper test (PN-94 R-65950) while determining the germination viability (1st date) and the germination capacity (2nd date) as well as the healthiness of the seeds. A significant differentiation of the effect of the extracts on vitality and healthiness of seeds was demonstrated depending on the origin of the extract (plant species), the way it was prepared and an interaction between these factors. The germination viability of seeds of brewing barley was influenced mostly by infusions from fruits of Coriandrum sativum, from seeds of Linum usitatissimum, from bark of Quercus robur, from roots of Levisticum officinale, from roots of Arctium lappa and from flowers of Verbascum thapsiforme. The most favourable activity on germination capacity was revealed using the infusions from seeds of L. usitatissimum, from roots of A. lappa, from fruits of C. sativum and macerations from roots of A. lappa, from fruits of C. sativum and from bark of Q. robur. The infestation of seeds by microflora was reduced by macerations from leaves of Betula verrucosa, from seeds of L. usitatissimum, from fruits of Juniperus communis, and infusions from flowers of Crataegus oxyacantha, from seeds of L. usitatissimum, from leaves of B. verrucosa. The most favourable impact on viability of the seeds of common barley was revealed for infusions from roots of L. officinale, from stigmas of Zea mays, from flowers of C. oxyacantha and macerations from flowers of Lavandula vera, from leaves of Mentha piperita and from roots of L. officinale. A positive effect on the germination capacity was exerted by infusions from the stigmas of Z. mays, from flowers of C. oxyacantha, from rhizomes of Acorus calamus, from bark of Frangula alnus, and macerations from bark of F. alnus, from leaves of M. piperita, from flowers of C. oxyacantha and from herb of Marrubium vulgare. The contamination of the seeds by microflora was reduced most effectively with infusions from young sprouts of Pinus sylvestris, from roots of Inula helenium, from leaves of Juglans regia, from herb of M. vulgare, from stigmas of Z. mays and macerations from rhizomes of A. calamus, from herb of M. vulgare, from leaves of J. regia, from seeds of L. usitatissimum and from flowers of L. vera.
W badaniach określono wpływ ekstraktu z grejpfruta i fungicydu Miedzian 50 WP na zdrowotność grochu oraz na kształtowanie się populacji mikroorganizmów w ryzosferze tej rośliny. Liczba roślin wyrosłych z nasion zaprawianych Grevitem 200 SL bywa zbliżona, a nawet przewyższa, ale nie statystycznie istotnie, liczbę roślin uzyskanych po zastosowaniu fungicydu Miedzian 50 WP. Wartość indeksu porażenia roślin była najmniejsza po wykorzystaniu Grevitu 200 SL, ale statystycznie nie różniła się od tej dla Miedzianu 50 WP. Z porażonych roślin izolowano m. in. Alternaria alternata, F. culmorum, F. oxysporum, F. solani, Phoma eupyrena, Pythium irregulare, Rhizoctonia solani i Sclerotinia sclerotiorum. Z ryzosfery izolowano m. in. Alternaria alternata, Fusarium spp., Gliocladium spp., Mucor spp., Penicillium spp., R. solani, S. sclerotiorum i Trichoderma spp. Liczebność bakterii ryzosferowych w kombinacjach z Grevitem 200 SL i Miedzianem 50 WP byáa istotnie większa, aniżeli w kombinacji kontrolnej. Odwrotna zależność wystąpiła w przypadku liczebność grzybów, ale statystycznie mniej grzybów wystąpiło w kombinacji z Grevitem 200 SL. Mikroorganizmy antagonistyczne dominowały w ryzosferze roślin wyrosłych z nasion zaprawianych Grevitem 200 SL.
In in vivo experiments the natural biologically active substances, which may reduce the application of chemical plant protection pesticides were examined. As dressing preparations water extracts (macerations, infusions) prepared from various parts of 39 plant species were used. For dressing non-disinfected seeds of two varieties of oat: Akt with naked seeds and Bajka with chaffed seeds were used. The experiment was conducted as a filter paper test, germination viability (I date) and germination capacity (II date) as well as healthiness of the seeds were determined. A significant differentiation of the effect of the extracts depending on extracts’ origin (plant), mode of their preparation and of interaction between these factors was revealed.
On wheat seeds harvested in Upper Silesia (south-western Poland) a large number of species of Fusarium were recorded. Fusarium avenaceum and F. culmorum were most commonly isolated. Flooding of fields for 1-7 days caused the increase of pathogenic Fusarium spp. in seed samples, especially F. culmorum. The infection of seeds caused a rapid decrease of their viability, and negatively affected health status of emerging seedlings in field conditions, in spite of performed chemical treatment.
A field experiment was conducted in the years 2003-2004 on a field of 10-years’ soybean monoculture, with naturally accumulated infection material in the soil. Soybean of ‘Mazovia’ was the object of studies. Before the sowing the seeds were dressed, and at the beginning of anthesis the plants were sprayed with biopreparations Polyversum, Biosept 33 SL and Biochikol 020 PC. The experiment considered the combination with dressing the seeds by means of Zaprawa Oxafun T (a.s. carboxine 37.5% + tiuram 37.5%) and spraying the plants with fungicide Bravo Plus 500 S.C. (a.s.: chlorotalonile 50%). The seeds that were not dressed constituted the control. During the vegetation the number and healthiness of soybean plants were determined twice (in the seedling phase and at anthesis), at the same time a laboratory mycological analysis was carried out. After the harvest the studies established the size and quality of the seed yield of soybean growing in particular experimental combinations. The best number of plants was obtained in the combination with Biosept 33 SL, slightly weaker after applying Polyversum or Zaprawa Oxafun T, and the worst in the control combination. Biosept 33 SL and the fungicide were the most effective in protecting the plants from infection by soil-borne fungi. Polyversum and Biochikol 020 PC also showed a good protective effect. The greatest seed yield was collected from soybean plants after applying Biosept 33 SL, while the lowest – from control plants. Soybean plants were mainly infected by Fusarium spp.,A. alternate, P. exigua, R. solani and P. irregulare. Those fungi were most frequently isolated from control plants, and the least frequently after applying Biosept 33 SL or Polyversum. The proportion of pathogenic fungi (P. sojae, Fusarium spp., S. sclerotiorum, P. exigua) in seed infection of soybean was the smallest in the combinations with Biosept 33 SL or Zaprawa Oxafun T, while the biggest in the control.
Influence of grapefruit extract (GE) on mycelial growth, spore germination, population density of Fusarium oxysporum f. sp. cyclaminis and F. oxysporum f. sp. dianthi and its effectiveness in the control of Fusarium wilt of carnation and Fusarium corm rot of gladiolus (F. oxysporum f. sp. gladioli) was studied. Amendment of PDA with 40 μg of GE/cm³ inhibited mycelial growth about 50%. Drenching of carnation with 165 μg of GE/cm³, immediately after planting, resulted in drastical decrease of colony forming units number of the pathogen and increased healthiness plant stand about 50%. Applied as gladiolus corm soak, GE at conc. 660 μg/cm³ decreased development of Fusarium rot of gladiolus clones at least twice.
This paper presents results of previous research, which may prove useful for the re-introduction of hypericum to crop farming. Climatic conditions for growing thisl herb in Poland are quite good. It requires fertile soil, with light or medium firmness (compactness), which is permeable and sufficiently wet, and has a slight acidic reaction. The forecrop is very important in this case. Hence, this paper evaluates its effect on the healthiness of plants and thus time-scale for plantation usage. The studied plantations were established after potatoes and wheat. It was observed that hypericum grew very slowly in the first year and was labour consuming due to weed control. A two- year old plantation gave dense herb growth independently of the forecrop. However, in the spring of the third year, hypericum sprouted irregularly. Herb plants in the after-wheat plantations showed retarded development in 39% and up to 34% of the plants in the after-potato plantation. Moreover, single stalks, decreased height or poor foliage was also observed. Laboratory diagnostic tests showed that stalk bases, root necks and main roots were intensively infested by the fungi that are pathogenic for most crops, i.e. the species of Botrytis, Fusarium, Phoma and Rhizoctonia.
This work presents the results concerning the yielding (time of ripening, weight of clusters and berries, fertility of vines, percent of soluble solids, taste of fruits), winter hardiness and susceptibility to fungal diseases (downy mildew, powdery mil­dew, grey mould and excoriose) of 25 selected cultivars for processing. The investiga­tions were carried out in the grapevine collection of the Research Institute of Pomol­ogy and Floriculture in Skierniewice, Poland. This grapevine collection was estab­lished in 1992, and it is consisted of 234 taxons. Assessed genotypes differed consid­erably in terms of the examined traits. Berries ripened from the second half of August ('Reform') until the second week of October ('V 71141', 'Sibera'). In the years 2005­2009, vines of the hybrid 'V 64035' and cultivars 'Seyval' and 'Cayuga White' were most productive. Berries of 'Delaware' were characterized by having the highest level of soluble solids. Vines of interspecific hybrids were less susceptible to frost damage and fungal diseases than cultivars of V. vinifera ('Chasselas Dore', 'Ortega'). Inter­specific hybrids 'Seyval', 'Bianca', 'Sibera', 'Marechal Foch', 'Rondo' and 'Regent' were distinguished as having the best yielding and highest quality of fruit from among the assessed cultivars, for commercial wine making. 'Aurore', 'Delaware', 'Cascade' and 'Golubok' were relatively reliable in yielding, and their grapes may be used for the production of juice and home wines.
One of the reasons lowering the esthetic appearance of a lawn are diseases caused by fungi. The study on seed material of 11 ornamental grass species was carried out in 2009 and 2010. 681 fungi isolates belonging to 22 species and non-sporulating forms were obtained over the two year study period. Among fungi pathogenic to grasses, species of the genus Fusarium were obtained especially in 2010. In that year, isolates of these fungi constituted 29.94% of all isolated colonies. Colonies of Alternaria alternata were also frequently isolated from the analyzed seed material of the ornamental grasses; in 2009 and 2010 its isolates accounted for respectively 25.36 and 12.57% of all isolates. The study on the health of ornamental grasses conducted in field conditions revealed the occurrence of plants with necrosis of the roots and lower stem internodes. The percentage of such plants ranged from 12% for Coix lacryma-jobi to 69% in the case of Festuca ovina. The mean values of the diseases indices were from 2.6 in the case of Coix lacrymajobi to 39.6 for Lagurus ovatus and they differed significantly. From the infected plants mainly species of genus Fusarium were obtained. Majority of isolates consisted F. oxysporum and also F. culmorum, F. crookwellense, F. graminearum, F. solani, F. avenaceum, F. sporotrichioides and species Bipolaris sorokiniana and Exserohilum pedicellatum were obtained. Studies on susceptibility of the seedlings of seven ornamental grass species to infection by Fusarium culmorum No. 34 and F. equiseti No. 62 were conducted in a growth chamber. The statistical analysis of disease indexes for plants that grew in the experimental combination with artificial infection of the subsoil with abovementioned strains as compared to the control indicated significant differences in all studied species in the case of F. culmorum and in 5 species in the experimental combination with F. equiseti. Strain Fusarium culmorum No. 34 proved to be the most pathogenic towards Festuca glauca (98.25), and F. equiseti No. 62 towards Phalaris canariensis (89.5).
Zinnia elegans is an annual plant recommended for cultivation for cut flowers as well as flowerbeds. This ornamental plant can be infected by: viruses, bacteria, phytoplasms and by a number of fungi species. Presented studies were conducted in 2006 in three localities of the Lublin region: in the vicinity of Zamość, near Krasnystaw and near Opole Lubelskie. Those studies included three cultivars of Zinnia elegans Jacq.: Golden Dawn, Lawa, Scarlet Flame as well as the mixed material of the cultivars belonging to the dahlia group. Six weeks after the sowing, the seedling’s healthiness was assessed. The proportion of seedlings with disease symptoms ranged from 7% to 44%. The main cause of root infection at the seedling stage proved to be the species of F. equiseti and S. sclerotiorum. Considerable amounts of B. cinerea and F. culmorum and A. alternata were also detected from diseased seedlings. Studies on the susceptibility of analyzed cultivars and the mixed material of elegans zinnia to infection by B. cinerea and F. avenaceum F. culmorum and F. equiseti were conducted in a growth chamber. Results obtained from this experiment confirmed considerable harmfulness of the species B. cinera and F. equiseti towards the seedlings of elegans zinnia, which can be reduced introducing less susceptible varieties to the cultivation. However, none of the studied varieties of elegans zinnia, only its mixed material, showed such properties.
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