In order to examine the risk of Anaplasma phagocytophilum infection in humans, 76 Ixodes ricinus ticks and 3 Dermacentor reticulatus removed from humans were examined for the presence of Anaplasma phagocytophilum by PCR targeting the16S rRNA gene. The average infection rate was 23.7% (18/76) in Ixodes ricinus ticks. A very high infection rate reaching 36.8% (14/38) was detected in female Ixodes ricinus, followed by males – 16.6% and nymphs – 10.5%. No infection was noted in larvae. All Dermacentor reticulatus also tested negative. The positive PCR products were sequenced and deposited in the Gen Bank (Accession No. DQ006828).
The purpose of this study was to specify the occurrence and prevalence of Babesia microti in hard ticks removed from dogs in Warsaw (central Poland). Among 590 collected ticks, 209 were identified as Ixodes ricinus, and 381 as Dermacentor reticulums. B. microti DNA was detected in 11 out of 590 (1.86%) samples of ticks. The DNA of the parasite was detected only in lysates from female I. ricinus ticks (11 out of 193; 5.7%). The result of this study is the first evidence of B. microti in I. ricinus ticks in Warsaw.
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