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The aim of the study was to evaluate the microstructure and elemental compositions of alpaca (Vicugna pacos) hair. The hair from eight alpacas that came from different parts of Poland were analyzed by scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM-EDX). A quantitative analysis was performed for nutritionally important elements (calcium and phosphorous); elements which are part of building blocks of hair structure (silicon and sulfur) and trace elements (copper, zinc, selenium). Moreover, elemental mapping was carried out. The SEM images showed that microstructure of hair is irregular and rough. The average diameter of hair is 32.27 ± 4.06 µm. The obtained results showed content of elements in hair and also their distribution in the structure of hair. These findings can be a good benchmark for further studies.
The aim of the present work was to evaluate the mercury content in hair of inhabitants of Wrocław, in southwestern Poland. On the basis of a questionnaire and analysis of hair by atomic absorption spectroscopy, it was possible to indicate the sources of mercury exposure. The mean mercury level in hair of the whole population (n=321) was 0.203±0.181 mg·kg⁻¹. The content of mercury in hair of subjects who consumed fish exceeded the upper limit of reference value, which was 0.397 mg·kg⁻¹. Subjects who declared consumption of fish, honey, and mouldy cheese, contained statistically more mercury: 60.5%, 35.4%, and 37.8%, respectively, than those who did not eat these types of food. It was noticed that there was no effect from the place of residence, the presence of pollution emitters, gender, age, weight, height, presence of amalgam fillings, hair dyeing, and smoking cigarettes on mercury content in hair.
Our study investigated the effects of dietary supplementation with zinc and polyphenol compounds, i.e. resveratrol and genistein, on the effectiveness of changes in the content of select elements (Zn, Mg, Fe, Ca, and P) in hair of rats with chemically (DMBA (7,12-dimethyl-1,2- benz[a]anthracene) induced mammary cancer. Regardless of the diet (standard; Zn; Zn+genistein) there occurred an increase in Fe and Zn content as well as a decrease in Ca, Mg, and P content in the hair of rats with mammary cancer in comparison with the content of those elements in healthy animals. Only in the group of rats supplemented with Zn and resveratrol were no changes in hair observed as compared with the control group, fed the same diet but without DMBA supplementation. The process of neoplasia in mammary tissue caused a number of changes in the concentrations of elements in hair. Certain dietary factors seem to have a significant effect on the distribution of elements in hair, but the reason for this phenomenon remains unknown.
The aim of the research was to develop a method for determining the species affinity of animal hair by determining its melting temperature. The research material was selected from hair samples of livestock and wild animals. We investigated 170 hairs of 16 animal species and humans. The hair melting point was determined by an automated system for melting temperature determination Opti Melt (MPA100). In the present study, we suggest that species affiliation can be effectively determined by examining physical properties of hair, in particular by determining its melting temperature. The hair melting temperatures for different animal species are in different ranges, but the initial melting points for hair of certain animal species are similar. For example, the initial melting point for cat, sheep, hare, rabbit, and rat hair ranged from 100 to 111°C, and the hair of the wolf, bear, and fox began to melt at 139.2 to 141.2°C. The results of this research make it possible to determine the species affinity of animals according to their hair melting temperature, which had not been done before. The proposed method yields rapid results and can be used as an auxiliary method in ambiguous cases in which data obtained by microscopic examination are insufficient. It will greatly facilitate the work of forensic specialists, enriching the arsenal of available methods
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Copper in human hair of Middle Pomeranian population

67%
The scalp hair has been suggested as a potentially valuable tissue, where short-term variation are averged out. Besides, hair has been demonstrated to be a major vehicle for the excretion of toxic metals, which concentration may be to 10 times higher than the amount found in blood or urine. Undoubtedly, hair analysis is a non-invasive method of diagnosis. The content of metals in hair depends on many factors. Hair samples (N = 416) were collected between the years 2003 and 2004 from Middle Pomerania (north Poland) population. Cu was determined by the electro-thermal graphite furnance AAS method. Hair from Middle Pomerania people contented in average 12.7 μg g-1 (range 5.2-32.0 μg g-1). The level of copper in hair was the function of age, sex, obesity, nutrition and disease, but was not the function of stature and weight. The obtained results were confirmed in comparison with the findings of other authors. Hair analysis might soon become a powerful diagnostic tool in monitoring of environmental exposure and studying the nutritional status of individuals.
The impact of the Gdańsk Phosphatic Fertilizer Plant “Fosfory” on citizens living near the plant was examined. Al, As, Au, Br, Ca, Cl, Cu, Dy, I, In, K, La, Mg, Mn, Mo, Na, S, Sb, Sm, U, V, Zn were determined by instrumental neutron activation analysis (IN AA) in hair and toenail clippings collected from 22 persons living in the neighborhood surrounding the plant and from 11 people from the control district. Essential differences in concentrations of elements for the citizens and the control group were evaluated using a non-parametric Mann-Whitney U-test. Significant differences between citizens of the factory neighborhood and the control group were found, for instance, for Mg, Sb, S and V, but it is hard to define if the factory impact exists, and whether it is the basis for these differences. The study was also undertaken to indicate a correlation between hair and nail element content and differences between concentrations of these elements considering sex.
The use of biological tissues as diagnostic tools of trace element level for recognising the disease status of an exposed human population is an important area of investigation. Quantitative analysis of 10 trace elements viz lead, calcium, magnesium, chromium, manganese, iron, nickel, copper, and zinc in the human body was performed using hair as biopsy material. In the present investigation, workers in a roadways workshop, locomotive workshop and Pb battery units were included as subjects. Head hair samples were collected from the nape region of the skull. Subsequent to sampling of hair, a questionnaire recommended by the World Health Organization was filled in order to obtain details about the subjects regarding occupation, duration of exposure, medical history, etc. Concentration of elements in hair was determined using an atomic absorption spectrophotometer. Workers under mental stress were treated as cases and workers without any symptoms of mental stress were chosen as controls. Significant difference in concentration of lead, calcium, magnesium, manganese, nickel, copper and zinc was found in hair of subjects with mental stress than those of controls. Concentration of chromium and iron was found higher in hair of subjects with mental stress but this difference was not significant with respect to controls.
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