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The objective of the work was to explain to what degree fluorine bioaccumulation is conditioned by the high level of industrialisation in Central Pomerania depending on the distance of a farm from the place of contamination emission in the trophic system: soil - plant - animal. Soil, feed, and horsehair and blood samples were taken from each level of the system. Material sampled from 48 crossbred horses was divided into three age groups: group 1 - horses aged 7 years and below, group 2 - horses aged between 7 and 15 years, and group 3 - horses aged over 15 years. Fluorine measurements in the soil, feed, and examined tissues were taken by means of an ion-selective electrode combined with an ORION ion meter. An elevated soil acidity in zone I, in the mouth of the Vistula River, stretching 50 km west of the place of contamination emission, increased the level of this element in the soils of the examined area, as well as in feeds offered to horses, compared with zone, II located in the mouth of the Leba and the Slupia Rivers, 80 to 100 km to the west of the place of contamination emission. A significantly higher average fluorine concentration in the blood and hair of horses raised in zone I, compared with zone II, was found. This concentration was the highest in the youngest animals. Fluorine accumulation in the blood and in the hair of horses raised in zone II was found to increase as the animals grew. Monitoring of fluorine bioaccumulated in soil, feed, blood, and the hair of horses may be a useful indicator of fluorine contamination in the natural environment.
The problem of relationships between the ultra-trace element content in a human organism and obesity has been poorly studied thus far. The primary objective of the current research has been to investigate the association between hair ultra-trace element content, body mass index and age in adult women. 1281 adult women participated in the survey. Hair ultra-trace element content (Ag, Au, Ga, Ge, La, Pt, Rb, Sb, Tl, W, Zr) was assessed by inductively coupled plasma mass spectrometry using a NexION 300D+NWR213 apparatus (Perkin-Elmer, USA). No significant association between the hair Ag, Au, Ga, Ge, La, and Pt content and body mass index (BMI) values was observed. The hair Rb levels in normal weight, overweight and obese women exceeded the respective values in underweight females by 33 (p < 0.001), 105 (p < 0.001), and 314% (p < 0.001). The hair Sb content in obese persons was 38 (p < 0.001), 38 (p < 0.001), and 22% (p = 0.022) higher in comparison to the values observed in underweight, normal weight and overweight subjects. A twofold increase in the hair Tl content was observed in obese females in comparison to the underweight (p < 0.001) and normal weight ones (p = 0.037). It has been observed that obese women were characterized by 33% higher hair W levels in comparison to the underweight (p < 0.001) and normal weight group values (p = 0.002). The results of correlation and multiple regression analyses partially confirmed these findings and indicated that hair Rb values were characterized by the closest association with the age and BMI. To our knowledge, it is the first report on a relationship between the hair ultra-trace element content and increased body weight. The data may be used as reference values of the content of ultra-trace element in hair of women with different body mass index. However, additional experimental and clinical studies are required to explore the mechanisms of such an association.
This paper discusses transfer cell wall deposition and architecture in various trap hairs of the carnivorous plant Utricularia intermedia. Scanning electron microscopy showed that the middle cells of both internal hairs and pavement epithelium hairs have reticulate-type wall ingrowths. The wall ingrowths of the middle cell of both quadrifids and bifids are very well developed. However, in middle cells of pavement epithelium hairs the level of development of wall ingrowths is not uniform. The presence of ruptured cuticles and wall ingrowths in these hairs suggests that water is transported by the pavement epithelium hairs from the trap to the external environment.
Results of a study on the content of cadmium and lead in the hair of persons in contact with these elements in their work place are presented. Hair was washed in order to remove exogenic contamination, and mineralized, and concentrations of the above elements were measured by differential pulse anodie stripping voltammetry (DPASV) in ammonium citrate buffer medium. The obtained results clearly point to absorption of cadmium and lead by the body. Measurements carried out on hair samples taken from persons with no contact with cadmium and lead compounds in their work places showed, in most cases, an absence of these elements.
Manganese is a component and cofactor for many important enzymes. In blood Mn is complexed to transferrin, and it quickly passes through the body to be extracted mainly in the bile and urine. Almost all Mn pool in blood is located in erythrocytes. Content of manganese in serum, erythrocytes, and hair of 26 men, workers of an airport, was determined. The control group consisted of administrative workers and the test group was composed of airplane servicemen. Hair samples of 0.5 g and 3-4 cm in length measured from the scalp were taken from some places on a head, washed with a detergent solution, rinsed with deionized water, acetone, and dried. Samples of blood were spun. All the samples were mineralized in a mixture of spectrally clean acids HNO3 and HClO4. Concentration of Mn was analyzed by electrothermal atomic absorption spectrometry GFAAS. The concentrations of Mn in the samples of erythrocytes were statistical significantly higher in the test group. In samples of hair, Mn concentrations were comparable between both groups of men. The coefficients of correlation between Mn concentrations in serum and hair, erythrocytes and hair, and between serum and erythrocytes did not imply significant correlations between Mn concentration in the analyzed clinical samples. In contrast, in the erythrocytes of men exposed on aviation fuel the content of Mn was significantly higher.
Sodium hypochlorite-digested material and scanning electron microscopy was used to study the morphology of wall ingrowths in pavement epithelium hairs of Utricularia species from the primitive section Pleiochasia (U. volubilis) and the advanced section Utricularia (U. stygia, U. intermedia). Wall ingrowths were reticulate-type in all examined species. Wall ingrowth development started with the formation of small papillae, which later lengthened and eventually fused and branched, forming a network. The sequence of wall deposition in plant hairs is given for the first time with SEM. The wall labyrinth in transfer cells of pavement epithelium hairs was found to be far from static. Different stages of wall ingrowth development were observed within the same cell.
The content of trace elements (Cu, Cr, Ni, Pb, and Zn) in hair and skin of silver fox (Vulpes vulpes) and arctic fox (Alopex lagopus) were determined. The content of elements were determined using inductively coupled plasma atomic emission spectrometry (ICP-OES). Samples of animal hair and skin were collected from two farms located in mid-western Poland: farm A, situated in a rural area and farm B, situated in a typically urbanized and industrial region. The influence of farm location on the concentration of Cr, Cu, and Zn in fur coat, and Cr and Ni in skin was observed.
There are some taxonomic uncertainties within the Turkish members of Satureja. It is extremely difficult to distinguish some Satureja species because of their great morphological similarity. Satureja species are used as herbal teas and spices, and for this reason they are important commercial and medicinal plants. In this study, the leaf anatomy and hair features of species were examined by LM and SEM. The investigated species can be divided into two main groups, as bifacial and equifacial leaves according to mesophyll structure. They can be secondarily divided into two types based on the midrib region in cross section, as projecting or nonprojecting. Thirdly, two main vascular bundle types can be identified in transverse section according to the presence or absence of sclerenchyma. All species have glandular, peltate and capitate, and nonglandular hairs and diacytic stomata, but the covering hairs differ between species. The leaves of fifteen Satureja L. species were studied in order to assess anatomical variations that may serve as distinguishing characters, and to evaluate their significance for the taxonomy of the genus.
The value of the routine analyses, applied until present, of whole blood, serum and urine for bioelements is limited. The blood mineral level does not often correspond to the content of minerals in the whole body because the composition of plasma results from supplementation of deficiencies by different homeostatic mechanisms. Moreover, the blood concentration of bioelements is relatively low and depends on a current diet, therefore the diagnostic value of such analytical results may be fairly small. Studies have shown that the analysis of hair and nails are an appropriate alternative for blood and urine tests or for biopsy. Chemical treatments in agriculture, animal production and food processing introduce many food contaminants into the food chain. Organic methods in agriculture are safer and therefore very important. Nutrition based on organically produced foods and anthroposophic lifestyle can play an important role in health prophylaxis The objective of this study was to determine correlations between concentrations of 29 major and trace elements in cow’s milk and hair. The experimental material consisted of 33 cows of Polish Holstein-Fresian (HF) breed from three dairy organic farms. All the farms were located in one climatic zone and under similar soil conditions. The cows were kept in traditional tiedup barns. The feeding was traditional, with ration components given separately. The cows were grazed from May to October. Depending on pasture yield and availability of other feeds, the feeding ration was supplemented with hay, straw, silage and cereals. Samples of milk and hair for analyses of minerals were collected in September, i.e. during the pasture feeding. The hair was taken from the poll. The concentration of Ca, K, Mg, Na, P, S, B, Ba, Co, Cr, Cu, Fe, Ge, I, Li, Mn, Mo, Ni, Se, Si, Sn, Sr, V, Zn, Al, As, Cd, Hg, Pb was determined. The content of toxic elements in milk was low and below the admissible level. The statistically significant positive correlations between concentration in milk and in hair were detected for such elements as Ba, Ge, Mo and Pb. In the case of major elements K and Mg and trace elements Al, As, Co, Fe, Hg, Se, Sr, positive correlations were observed but they were not statistically significant. Negative correlations occurred for such elements as Cr, Cu, I, Li, Ni, S, Si, Sn, V and Zn. It was only for V and Zn that they were statistically significant. Very low values (near zero) of coefficient r were observed for Ca, Cd, Li, Mn, Na, Ni, P, S and Sn. It seems that broader investigations of mineral composition of cow’s hair could be useful for establishing reference values for some elements and would make a contribution to better animals’ welfare.
Samples of hair collected in 2004-2007 from 416 persons living in Central Pomerania were analyzed. The subjects donating hair represented a vast spectrum of age, from a tenmonth- old child to a 75-year-old person. The subjects were selected randomly. Lead and cadmium were determined by atomic absorption spectrophotometry using an ASA-3 spectrometer. The average content of the metals in the hair samples was 3.20 μg g–1 (Pb) and 0.284 μg g–1 (Cd). The highest concentration of lead in human hair (about 3.88 μg g–1) was determined for the age group 61-75 years, and that of cadmium (0.406 μg g–1) – for the age group 26-50 years. The lowest concentrations of these metals in human hair (2.07 and 0.152 μg g–1, respectively) were determined for the age group of 0-15 years. Most hair samples (50%) contained 2.01-4.00 μg g–1 Pb, while 45% of the samples contained 0.001- -0.300 μg g–1 of cadmium. Studying the dependence of the content of lead and cadmium in hair on the gender of subjects, it was discovered that in all age groups males had more lead and cadmium (3.79 and 0.334 μg g–1, respectively) than females (2.63 μg g–1 and 0.236 μg g–1). This study has also demonstrated that the environment affects the content of the analyzed metals in hair. The average value of lead and cadmium concentrations for people living in the country were 2.39 μg g–1 for Pb and 0.214 μg g–1 for Cd, while for the people living in towns and cities, the respective values were 4.17 and 0.361 μg g–1. The present study has demonstrated how nutrition affects lead and cadmium content in human hair. Among the subjects, 17% had been on some kind of a diet, predominatly easily digestible and light foods. The lowest content of these metals (on average, 2.08 μg g–1 Pb and 0.141 μg g–1 Cd) was found in hair of people on a diet, while the hiest levels (3.54 μg g–1 Pb and 0.315 μg g–1 Cd) were determined in people who did not limit consumption of meat and dairy products. Among the analyzed population, 241 persons suffered from chronic disease. The average content of lead and cadmium in hair of healthy subjects was 3.05 μg g–1 Pb and 0.257 μg g–1 Cd, but in patients suffering from arthrosclerosis, allergy and hyperplasia prostate the levels of lead and cadmium in hair reached the upper values of the se limits. Hair of the patients who suffered from cardiovascular disease showed deificiency of these metals (on average, 1.73 μg g–1 Pb and 0.182 μg g–1 Cd).
The aim of the present work was to evaluate the mercury content in hair of inhabitants of Wrocław, in southwestern Poland. On the basis of a questionnaire and analysis of hair by atomic absorption spectroscopy, it was possible to indicate the sources of mercury exposure. The mean mercury level in hair of the whole population (n=321) was 0.203±0.181 mg·kg⁻¹. The content of mercury in hair of subjects who consumed fish exceeded the upper limit of reference value, which was 0.397 mg·kg⁻¹. Subjects who declared consumption of fish, honey, and mouldy cheese, contained statistically more mercury: 60.5%, 35.4%, and 37.8%, respectively, than those who did not eat these types of food. It was noticed that there was no effect from the place of residence, the presence of pollution emitters, gender, age, weight, height, presence of amalgam fillings, hair dyeing, and smoking cigarettes on mercury content in hair.
The study of content of K, Mg, Ca, Na, Zn, Cu, Fe, Li, and Pb in human hair was carried out on 300 individuals in the years 1996-2002. The patients were from Lublin, Zamość, Łabunie, Krasnystaw, Ostrów Krupski, and Zwierzyniec. The samples of hair to be examined for the content of Mg, K, Na, Zn, Cu, Fe, Li, and Pb were collected from 79 healthy controls, 98 mentally handicapped, 81 psychiatric patients and 42 somatic patients. The content of particular elements in hair was determined using atomic absorption spectometer type AAS-3 (Carl Zeiss), attached to a microcomputer type 113 MPC/38 C and Spectr AA 880 by Varian. The results of the study of hair revealed the positive correlation between Mg and Pb (Rs=0,49, p=0,000024) and between K and Na in healthy controls. Negative correlation was revealed between Mg and K in healthy controls and between Mg and Ca in all studied patients.
Many bats are specialized to detect and capture arthropods from vegetation. As echoes from sitting arthropods and vegetation background overlap strongly, it is difficult for those bats to detect prey by echolocation alone. Within the largest genus of bats, Myotis, at least three species from different sub-clades show a characteristic fringe of hairs on the trailing edge of their uropatagium. All three species are capable of gleaning arthropods from vegetation with this tail membrane. Phylogenetic analyses strongly suggest that these specializations evolved convergently. Therefore, one can hypothesize that the hairs at the rim of the tail membrane have an important tactile and/or mechanical function for gleaning prey from substrate. To assess this question, we used light microscopic techniques to investigate the morphology and innervation of the bristle-like hair fringe, and for comparison, the structure of sensory mystacial vibrissae in Myotis nattereri. The results revealed that the fringe possesses two types of hair: larger guard hairs and smaller vellus hairs. Both hair types are well innervated underneath their sebaceous glands. They are encircled by a piloneural complex, which functions as a stretch and tension receptor. Although the bristle-like hairs are clearly not vibrissal follicle-sinus-complexes, their position, morphology and innervation strongly support a sensory function for prey detection and capture. An additional mechanical function, e.g., brushing prey off substrate, is plausible.
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The aim of this work was the assessment of the age, sex, way of nutrition, environment and health state influence on zinc content in hair of population living in the Middle Pomerania. Within years of 2005-2007 hair coming from 416 persons from a ten months old child to a 75 years old person was analyzed. All people were divided into four age groups: children (0-15 years), youth (16-25), adults (26-50) and the elderly (51-75). 3-centimeters long hair (counting from the skin) were put to chemical analyze. Then dried samples were weighed and mineralized with mixture of nitric and tetraoxochloric acides (mixed in proportion of 5:1). Zink determination was done by method of atomic absorption spectrophotometry using spectrometer ASA-3. Average zinc content in hair of researched population amounted to 167±58 7g g-1. Least of all zinc was ascertained in children hair (av. 125 7g g-1), and most of all in youth (194 7g g-1). In all age groups females had more of this metal (av. 180 7g g-1) than males (156 7g g-1). Average value of zinc for people living in a village was 147 7g g-1, and living in towns and cities 181 7g g-1. The least of zinc we have found at persons being on the meat- or milk-free diet (av. 157 7g g-1) and the most at persons that did not restrict in eating meat and milk products (197 7g g-1). The persons suffering from cardiovascular disease or hyperplasia prostate had adequately 221 and 195 7g g-1 Zn, considerably above the level in hair of without symptoms diseases persons (156 7g g-1). Smaller content of this element had persons suffering from atherosclerosis or hypertension (adequately 132 and 142 7g g-1).
Our study investigated the effects of dietary supplementation with zinc and polyphenol compounds, i.e. resveratrol and genistein, on the effectiveness of changes in the content of select elements (Zn, Mg, Fe, Ca, and P) in hair of rats with chemically (DMBA (7,12-dimethyl-1,2- benz[a]anthracene) induced mammary cancer. Regardless of the diet (standard; Zn; Zn+genistein) there occurred an increase in Fe and Zn content as well as a decrease in Ca, Mg, and P content in the hair of rats with mammary cancer in comparison with the content of those elements in healthy animals. Only in the group of rats supplemented with Zn and resveratrol were no changes in hair observed as compared with the control group, fed the same diet but without DMBA supplementation. The process of neoplasia in mammary tissue caused a number of changes in the concentrations of elements in hair. Certain dietary factors seem to have a significant effect on the distribution of elements in hair, but the reason for this phenomenon remains unknown.
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