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In boreal and temperate forests fungi play a particularly important role, since most trees form a symbiotic relationship with many species of ectomycorrhizal (ECM) fungi, providing them with assimilates in exchange for minerals. Mycorrhiza is considered one of the most significant factors affecting functioning of forest ecosystems, and in particular the processes of carbon cycling and storage. ECM fungi are involved both directly through carbon accumulation in the mycelial system, and indirectly through their influence on tree biomass production and organic matter decomposition. The amount of carbon transferred to ECM fungi usually varies from 10 to 25 or even 50% of the host's net photosynthesis, thus they are a group of organisms that significantly affect carbon flow into the soil. Most of that carbon is built into the mycelial system and its structures (fungal parts of ECM roots, extramatrical hyphae and rhizomorphs, sporocarps, etc.). Carbon allocation to the underground part of trees, and thus to ECM roots, changes with stand age and stand development phase. The biomass of active ECM roots and mycelium usually reaches its maximum in young stands, in the canopy closure phase; frequently, this is also true for the standing biomass of fruit bodies. A large share of ECM sporocarps in the forest carbon budget and high levels of ECM vegetative mycelium respiration are considered to be among the main pathways for the release of CO2 from forest soil, indicating a significant role of ECM fungi in fast carbon flow via forest ecosystems. On the other hand, dead ECM fine roots and extramatrical mycelia are a very rich and important pool of sequestered carbon in the soil.
An assessment of ectomycorrhizal fungi associated with Pinus sylvestris L. growing on abandoned post−agri− cultural soil was performed. The seedlings were growing at three different treatments of soil amendments (harvest residue, bark compost and sawdust). As a control treatment, the soil without any amendments was used. The comparison of ectomycorrhizal structure done ten years after the application of organic substrates showed no significant changes in species richness level. The most frequent taxa, irrespective of the treatment, was Wilcoxina sp. The result seems to be connected with high nitrogen level in the soil. Ectomycorrhizae of Cortinarius sp. and Pinirhiza spp. dominated in all treatments. The results showed that species richness and abundance of live and dead mycorrhizae depend on soil conditions, which are similar on treatment and control plots.
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The paper provides the review of research on interaction in dual cultures in vitro (fungus and plant tissue culture of its host plant) with marking test results in this area in order to show usefulness of this method in testing pathogenicity and the possibility of its use in forest practice
Pedunculate oak (Quercus robur L.) is among the major managed tree species in Europe. In Poland, oak woodlands cover 6.9% of forest area. Furthermore, the significance of native oaks for Polish forestry is rising because the Polish National Forest Strategy predicts a growth of forest cover from 28.5% to 33% by year 2050 with the primary focus on the increase in contribution of deciduous tree species (from 22% to 33%). Therefore, for establishment of new stands, oak seedlings are grown for 2−3 years in forest nurseries. For optimal growth and development all European oak species are critically dependent on ectomycorrhizal fungi. The objective of this study was to describe the temporal dynamics of ectomycorrhizal community of pedunculate oak seedlings during the first year of growth in bare−root forest nursery. Experiment was conducted in forest nursery Miranowo (western Poland). The species structure of an ectomycorrhizal community was assessed every 10 or 15 days from May till October. Mycorrhizal colonization of tested seedlings changed from 0 to 85% depending of the time of sampling. Based on combination of morphological and molecular techniques, nine ectomycorrhizal fungal taxa were distinguished. The first ectomycorrhizas formed by Scleroderma verrucosum were detected 50 days after acorns sowing. In the next sampling days ectomycorrhizal community was dominated consecutively by Inocybe curvipes and Hebeloma sacchariolens (with maximum of abundance 60.98 and 75.55%, respectively). The obtained results were strongly influenced by the infection with the fungal biotroph Erysiphe alphitoides and resulted in a dramatic reduction in the abundance of living ectomycorrhizas down to 7.5%. Our investigation revealed that ectomycorrhizal fungal community of pedunculate oak seedlings during the first year of growth in bare−root forest nursery is rich in terms of number of species and a temporal changes are very dynamic. Thus we suggest, that in case of pedunculate oak artificial inoculation in general is not necessary for oaks seedlings in nursery practice.
The effects of tree genotype on below−ground ectomycorrhizal fungal communities of 40 years old European larch from Kowary, Bliżyn, Świętokrzyski National Park and Szczytna Śląska origins was investigated. The research was carried out in the provenance trial in the Experimental Forestry Unit in Krynica Zdrój (Beskid Sądecki Mountains, S Poland). Ectomycorrhizal fungal communities were assessed by a combination of morphological and molecular techniques. In total 22 fungal taxa has been distinguished (from 17 to 20, depending on larch provenance). Thirteen ectomycorrhizal fungal taxa were common to all analysed origins. The mean taxa richness, Shannon diversity and Simpson dominance coefficient did not differ significantly between provenances. Based on fungal taxa composition (Jaccard coefficient) tested larch origins were characterized by a high level of similarity of ectomycorrhizal fungal communities. Six detected fungal taxa have not been previously reported as symbiotic partners of European larch.
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