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Fat-inducing transcript (FIT) are endoplasmic reticulum-resident membrane proteins that induce lipid droplet accumulation. It plays a crucial role in the fundamental process of storing fat. In this study, applying the PCR-SSCP and DNA sequencing methods, polymorphism of the FIT gene were detected. A total of 708 individuals from four Chinese cattle breeds were examined. The results showed that only P5 locus had two SNPs, resulting in a synonymous mutation (NM_001103095: m.199G > T resulting In L124L) and a missense mutation (NM_001103095: m.434G > T resulting in V176L). The associations between polymorphic loci and selected growth traits of indigenous Nangyang cattle were analysed,and significant associations were found in body weight at the age of 12 months and mean daily live weight gain. The body weight at month 12 of life and mean daily live weight gain of individuals with genotype AA were by 3.75% and 4.88% higher than of those with genotype AB, respectively. Hence, it was suggested for the first time, that genotype AA could be regarded as molecular marker for superior body weight and daily live weight gain in Chinese Nanyang cattle.
LHX4 is a LIM homeodomain transcription factor involved in pituitary ontogenesis. Some mutations of LHX4 are associated with combined pituitary hormone deficiency that affects growth and development in animal. The objective of this study is revealing to these polymorphisms of in LHX4 gene exons 1, 2, 3 in 820 Chinese cattle and to assess possible association of polymorphisms in LHX4 gene with growth traits in NY breed. The PCR-SSCP and DNA sequencing of LHX4 gene showed five novel synonymous mutations: (GenBank: NW_001493442.2: g.35143G >A, g.35152C>T, g.35212C>T, g.35230G>A, g.35233T>C). Genotype MM (GG-CC-CC-GG-TT) and haplotype M (G-C-C-G-T) were dominant in the four breeds, and genotype frequencies of LHX4 in the four cattle populations agreed with Hardy-Weinberg equilibrium (P>0.05), although their frequencies significantly differed among the four analyzed populations (***P<0.001). The association analysis showed that individuals with genotype MM had greater body weight than those with genotype MN (*P<0.05) at eighteen months of age in Nanyang cattle.
A polymorphism within exon 2 of the 2, 4-dienoyl-CoA reductase gene (DECR1) was investigated by PCR-SSCP in 228 Shanxi White pigs. An association between the DECR1 polymorphism and growth traits in Shanxi White pigs was determined with an univariate animal model. The polymorphism was found within exon 2 of the DECR1, giving rise to genotypes AA, BB or AB. This polymorphism exhibited a significant effect of generation and sex (P<0.05, P<0.01) on growth traits and backfat thickness. At the age of six months BB pigs showed the body weight and chest girth higher than AA and AB animals. However, the polymorphism revealed no significant effects on other growth traits (P>0.05) though a trend of BB>AB>AA was showed.
The objective of the study was to determine beef production traits of dairy and dairy × beef breed crossbred heifers. The data from Finnish slaughterhouses included observations of 14 221 Nordic Red (NR), 6 348 Holstein-Friesian (Hol), 1 626 NR × Aberdeen Angus (NR × Ab), 1 136 NR × Blonde d’Aquitaine (NR × Ba), 802 NR × Charolais (NR × Ch), 487 NR × Hereford (NR × Hf), 3 699 NR × Limousin (NR × Li), 827 NR × Simmental (NR × Si), 531 Hol × Ab, 467 Hol × Ba, 438 Hol × Ch, 186 Hol × Hf, 1 249 Hol × Li and 393 Hol × Si heifers. Crossbreeding with late maturing beef breeds (Ba, Ch, Li, Si) had favourable effects both on daily carcass gain and carcass quality traits (conformation, proportion of high value joints) of the progeny when compared to purebred dairy heifers. No advantages in proportion of high value joints seemed to be obtained by crossbreeding dairy cows with Ab or Hf breeds, while the improvements in daily carcass gain and carcass conformation score were intermediate compared to the late maturing crossbreds.
The nucleophosmin 1 gene (NPM1) encodes a multifunctional nucleolar phosphoprotein that plays a crucial role in the control of various aspects of cell growth and homeostasis. In this study, the coding region of the NPM1 gene was screened in 1035 individuals of 4 Chinese cattle breeds by DNA sequencing and Polyacrylamide gel electrophoresis. A novel 12-bp deletion mutation was identified in the coding region of the NPM1 gene. The PCR products of primer NPM1-P2 exhibited 3 genotypes and 2 alleles: 178 bp (denoted as W) and 166 bp (denoted as D). Genotype DD and allele D were predominant in the studied populations. Association analysis with growth traits in the Nanyang breed (N = 265) showed that the animals with genotype DD had significantly greater birth weight, body weight, body length, and heart girth than those with genotype WD (P <0.01 or P < 0.05) at birth and after 6 months and 12 months, but not at 18 and 24 months of age. Results of this study suggest that the NPM1 gene is a candidate gene for growth traits in cattle.
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