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The main objective of this study was to determine the allelopathic impact of dominant species (Calamagrostis epigeios, Rubus idaeus and Chamaenerion angustifolium) of clear-cuttings of Scots pine (Pinus sylvestris L.) forests (Vaccinio-myrtillo Pinetum) on test species germination emphasizing the forest ecosystem establishment. Aqueous extracts of roots and shoots were produced at different growth stages and assayed on germination. Additionally, total concentration of phenols was evaluated photo spectrometrically. Extracts of shoots more strongly inhibited germination than those of roots of all investigated species. The strongest inhibitory effect on germination and the highest phenol contents were documented during the flowering stage rather than in spring and autumn. Accordingly, to mean germination data the declining phytotoxicity sequence of the species was determined: R. idaeus > C. epigeios > Ch. angustifolium. Hence this study implied in allelopathic activity of species a potential suppressive factor that could influence germination and forest regeneration, but field condition studies are necessary.
The main purpose of this study was to examine the influence of pyrethroids, such as deltamethrin, cypermethrin and bifenthrin on the growth and the number of ρ-mutants in the cells of Saccharomyces cerevisiae yeast. SP-4 Mat alpha leu1 arg4 yeast strain was used as a standard strain for experiments. The cells were grown on a standard YPG liquid medium, under aerobic conditions until they reached the logarithmic or stationary phase of growth. Different concentrations of pyrethroid were added to the medium and the cells were incubated for 2 h. The survival rate of the cells was determined by diluting the cells and plating them on YPG Agar plates. The number of ρ-mutants was determined by examining the number of cells that hadn’t grown on YPG medium from among all surviving cells. As far as the investigated forms of pyrethroids are concerned, deltamethrin was the most toxic to yeast cells. It was also observed that low amounts of pyrethroid caused a greater destruction of cells at the logarithmic than at the stationary stage of growth. The influence of pyrethroid on the frequency of mutation of mitochondrial DNA of yeast cells was also studied. It was observed that after incubation with pyrethroid addition the frequency of ρ-mutation increased, especially at the logarithmic stage of growth.
In arid and semi-arid ecosystems, shrubs have an important effect on neighboring plants. However, little is known about the interaction of herb growth stages and shrub location on herb performance. We selected Reaumuria soongorica, (Pall.) Maxim a shrub dominant in the semiarid region of northwest China, to determine whether (1) shrubs facilitate or have negative effects on neighbouring herbaceous vegetation, and (2) such effects vary with herb growth stage and with shrub orientation relative to herbs. The presence of herbaceous plant species, plant density, plant height, and percent cover were determined along 2 m long transects spreading in four directions from the base of shrub – east (transect E), west (transect W), south (transect S), and north (transect N); this was repeated for three growth stages (in May, June and July). Results indicated that the effects of R. soongorica on neighboring herbs in different growth stages were similar. Species number of herb-layer plants tended to increase from beneath the canopy to the opening, but plant density, cover and plant height decreased with distance away from shrub base. The presence of R. soongorica had positive effects on density, cover, and plant height, and negative on the number of herbaceous species during the entire growing season. Herbaceous plants growing on transect N under the shrub canopy had significantly higher density and percent cover than those growing in other directions. Biomass of herbs on transect N grown under the shrub canopy was higher than that of herbs on other transects. We concluded that shrub effects on neighbouring herbaceous vegetation were closely related to the shrub orientation relative to the herbs. Therefore, using shrubs as nurse plants for grass-growing must consider the relative placement of shrubs.
Winter oilseed rape is the key oil crop in temperate climate regions of the world. Yield of seeds depends the most on nitrogen management throughout the season. The evaluation of N status in the oil-seed rape canopy was studied in 2008, 2009, and 2010 seasons. The one factorial experiment to verify the formulated hypothesis, consisting of six treatments, was as follows: absolute control (AC), NP, NPK, NPK + MgS - 1/3 rate of total planned rate applied in Spring (NPKMgS1), NPKMgS - 1.0 rate in Autumn (NPKMgMgS2), NPK+MgS - 2/3 in Autumn + 1/3 in Spring (NPKMgS3). Plant samples were taken at three stages: i) full rosette (BBCH 30), ii) the onset of flowering (BBCH 61), iii) maturity (BBCH 89). The total plant sample was partitioning in accordance with the growth stage among main plant organs such as leaves, stems, straw, and seeds. Yield of biomass, nitrogen concentration and content were determined in each part of the plant. The study showed an existence of two strategies of dry matter and nitrogen accumulation by oil-seed rape throughout the season. In 2008, and partly in 2010 revealed the strategy of yield formation relying on relatively slow, but at the same time a permanent increase in nitrogen and biomass accumulation during the season. This strategy resulted in a high seed density in the main branch. The size of this yield component significantly depended on nitrogen content in leaves at the onset of flowering. In 2009 revealed the second strategy of yield formation by oilseed rape. Its attribute was a very high rate of nitrogen accumulation during the vegetative growth, resulting in a huge biomass of leaves at the onset of flowering. This growth pattern, as corroborated by yield of seeds, was not as effective as the first one. The main reason of its lower productivity was the reduced seed density, especially in pods of the secondary branch, resulting from an excessive nitrogen content in leaves at the onset of flowering. Irrespectively on the strategy of nitrogen management by the oilseed rape canopy, the best predictor of the final yield was nitrogen content in seeds. It can be concluded that any growth factor leading to the nitrogen sink decrease, such as reduced plant density and/or disturbed N management throughout the season, can be considered as a factor negatively impacting yield of seeds.
An optimum plant crop nutrition status during critical stages of growth is one of the most important factors shaping their ability to yield. It has been assumed that plant nutrition during the spring wheat stem elongation stage has a significant influence on the growth and yield of this crop. In Poland, potassium is a critical nutrient for plant growth and yielding. In order to verify this hypothesis, three series of one factorial experiment were conducted in 2003-2005 that involved reduced rates of potassium applied to cv. Zyta winter wheat. The following rates of potassium were applied: 0, 25, 50 and 100 kg ha–1. The assessment of wheat nutritional status was conducted at the beginning of stem elongation (BBCH30/31) using a German model called PIPPA. It had been hypothesized that this particular stage was decisive for wheat growth and yielding. Wheat plants showed deficiency in Ca, K, P and N irrespective of the applied potassium rate. Calcium and potassium deficiencies were crucial for the final grain yield. However, the relationships that occurred between nitrogen and main nutrients, i.e. pairs of nutrients such as N: P, N: K, N: Ca, showed a much better prognostic value, i.e. the relationship with grain yield, than the levels of nutrients in separation. The nitrogen content in leaves at the beginning of shooting showed its limiting effect on grain yield, provided that Ca was deficient, an event which appeared when N: Ca was wider that 34: 1. The same correlation was noticed for potassium, but there the excess of nitrogen revealed its harmful effect when the N:K ratio was above 1.0.
An attempt was made to optimize the minimum required inoculation load of the weevils on three growth stages of waterhyacinth, based on reduction of fresh biomass, number of leaves and ramifications. The small growth stage was controlled earlier than the waterhyacinth of middle growth stage, corresponding to the increase in number of weevils per plant. The large plants could not be controlled even with the inoculation pressure of 20 weevils per plant because of high growth rate. This study suggests that Neochetina spp. has the potential to keep the population of the macrophyte at a subeconomic density, through a basic inoculation load of weevils in due course of time.
A high yield of oilseed rape can be achieved provided an adequate supply of magnesium and sulfur in critical stages of yield formation. The magnesium status in canopy was studied in the 2008, 2009 and 2010 growing seasons. A one factorial experiment consisting of six treatments, set up to verify the research hypothesis, was as follows: control (C), NP, NPK, NPK+ MgS - 1/3 of total planned dose applied in spring (NPKMgS1), NPK+1.0 MgS dose in autumn (NPKMgMgS2), NPK+MgS - 2/3 in autumn + 1/3 in spring (NPKMgS3). Plant samples were taken at three stages: rosette (BBCH 30), the onset of flowering (BBCH 61) and maturity (BBCH 89). An entire sample was partitioned in accordance with the growth stage into main plant organs: leaves, stems, straw and seeds. The yield of biomass, magnesium concentration and its content was determined in each part of the plant. The magnesium concentration in leaves at the onset of flowering can be used as the first predictor of yield. The predictive strength of the magnesium content in seeds as the final yield predictor corroborated the hypothesis of the importance of magnesium for the seed sink build-up. An analysis of relationships between the magnesium content in plant parts during the growing season and yield of seeds can be used to make an ex-post analysis of factors disturbing the development of yield structural components. The main cause of yield reduction in 2009 as compared to 2008 was the insufficient supply of magnesium to vegetative organs of oilseed plants since the onset of flowering. It was documented that the degree of magnesium supply to a growing silique is critical for the seed yield performance, as noted in 2008. It was also found that any disturbance in the magnesium supply to oilseed rape since the onset of flowering led to reduction in the seed density, which in turn decreased the magnesium seed sink capacity, as the study clearly demonstrated.
Density and diversity of Fusarium species and other fungi associated with rhizosphere and rhizoplane of lentil and sesame plants at three different growth stages were investigated. Sixteen species of Fusarium were isolated from rhizosphere (13 species) and rhizoplane (11) of both plants studied. In lentil, 11 species were recorded from its rhizosphere (9 species) and rhizoplane (8). Fusarium species associated with lentil rhizoplane gave highest number of propagules at the first stage of plant growth while the ones of Fusarium associated with the rhizosphere produced the highest number at the second stage of growth. F. solani was the most common in the three growth stages. In addition, of two growth stages, F. culmorum and F. tricinctum were isolated from the rhizosphere while F. nygamai and F. verticillioides from the rhizoplane. The other species were recorded from only one growth stage of lentil plant. In sesame plants, rhizosphere yielded nine Fusarium species while rhizoplane gave only six from the three stages investigated. Stage I of sesame rhizosphere possessed the highest colony forming units of Fusarium. As the case for lentil, F. solani was the most common species in sesame rhizospere and rhizoplane. F. verticillioides and F. nygamai (in three different growth stages) followed by F. oxysporum and F. tricinctum (in two growth stages) were recorded using the dilution-plate and/or soil-plate methods from sesame rhizosphere soils. Rhizoplane Fusarium species of sesame plants were isolated at the three different growth stages with almost equal number of colony forming units. F. poae came after F. solani in its frequency since it was recovered from two growth stages. Several of the isolated species are well-known as pathogens to many cultivated plants. To the best of our knowledge, three species are recorded here for the first time in Egypt from the rhizosphere (F. acutatum), rhizoplane of sesame plants (F. longipes) and from rhizosphere of both lentil and sesame and rhizoplane of lentil (F. nygamai).
The resistance of winter wheat varieties to Puccinia recondite f. sp. tritici was investigated at the Lithuanian Institute of Agriculture during 2001–2003. Effectiveness of resistance genes was investigated at seedling, tillering and adult plant stages. Virulence tests done during the 2000–2003 period showed that the majority of Lr genes used in European wheat were not sufficiently efficient. However, testing of cultivars at the first leaf stage revealed that the Lr37 gene in combination with the other genes was very effective. The experimental cultivars were sown in 2 times: in autumn and spring, without vernalization. The main task of spring-sown nursery was to improve the effectiveness of the experiment and investigate the effect of different Lr genes of non-vernalized plants at tillering growth stage. The Lr37 gene was found to be the most effective at both adult plant stage and tillering growth stage. Disease severity and plant resistance type at tillering stage were stable in all experimental years, which is important for the breeding program. The investigations revealed that the correlations between resistance at seedling and the other two stages were up to r = 0.81 (significant at p = 0.01**). The correlations between leaf rust severity and varietal resistance type at tillering were very high (r = 0.86–0.91**) in the same year. The correlation of leaf rust severity at adult plant stage was strong (r = 0.78**) between 2001 and 2002, but too low for reliable selection of resistant cultivars in the other years. Spring-sown nursery was complementary to collect resistance data in the years unfavourable for leaf rust development.
Optimal nutrition of cultivated plants at critical growth stages is of great importance for the achievement of full crop yield potential. The aim of this study was to assess the maize yield response and plant nutritional status at a critical stage of growth (BBCH 17) under the most favourable and reduced fertilization with phosphorus and potassium. It was assumed that the nutritional status of maize at BBCH 17 stage significantly influenced the plant growth and yielding. The hypothesis was tested in a one-factorial trial, carried out on the maize variety Veritis in 2007-2011, which was a part of a long-term study started in 2000 according to a randomized complete block design. The factor tested comprised different phosphorus and potassium doses applied at constant levels of nitrogen and magnesium fertilization. The yields of maize significantly differed between the treatments and in relation to the control. In each year, maize responded with a lower yield to the no-phosphorus treatment when compared to the no-potassium treatment. Irrespective of the fertilization variants, the content of the nutrients tested (except iron) was below the standard value. A significant relationship was shown between the nutritional status of maize at the stage of 7 leaves unfolded (BBCH 17) and grain yield. The coefficients of determination ranging from 59% to 94% showed that, irrespective of which treatment was applied, the mineral nutrient content in maize leaves at BBCH 17 stage had the strongest influence on the maize yield.
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