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Shoot tips of Tibouchina urvilleana Cogn. were cultured 4 weeks in vitro in modified Murashige and Skoog (MS) [1962] medium supplemented with growth retardants: paclobutrazol – 0.1, 0.5, 1.0, 5.0 mg·dm⁻³, flurprimidol – 0.1, 1.0, 5.0 mg·dm⁻³, chlorocholine chloride (CCC) – 5.0, 50.0, 250.0 mg·dm⁻³. Explants cultured on the medium without growth substances were used as a control. Rooted microcuttings were transferred to the greenhouse and transplanted into a mixture of 1 peat : 1 perlite, where they were grown for 5 weeks. Plants were then cultivated in a peat substrate during another 5 weeks. Acclimatization of rooted shoots in the greenhouse was affective in 92.5–100%. The survival of plants was lowest when microcuttings were previously cultured on medium with flurprimidol at 5.0 mg·dm⁻³. Cultivation of Tibouchina urvilleana shoots in vitro on media with various growth retardants had a significant effect on the further growth of plants ex vitro. Paclobutrazol and flurprimidol at 5.0 mg·dm⁻³ inhibited very strong growth of plants after 10 weeks of growth ex vitro.
The consequent effect of growth retardants: paclobutrazol – 0.1, 0.5, 1, 5 mg l⁻¹, flurprimidol – 0.1, 1, 5 mg l⁻¹, chlorocholine chloride (CCC) – 5, 50 mg l⁻¹ on the growth and development of Tibouchina urvilleana Cogn. in vitro was examined. Paclobutrazol at concentration of 1 or 5 mg l⁻¹ and flurprimidol at 5 mg l⁻¹ inhibited the growth of shoots significantly. It was noted that shoots originating from these media had thicker, straighter stems and shorter internodes. The stimulating influence of CCC at 5 mg l⁻¹ on the elongation of the main shoot was observed. Axillary shoots formation was the best when shoots were derived from the medium supplemented with flurprimidol 1 mg l⁻¹. Paclobutrazol at 0.5 and 1 mg l⁻¹ and flurprimidol at 5 mg l⁻¹ influenced the formation and fresh weight of roots increase, but flurprimidol was more effective. Growth retardants (except paclobutrazol at 5 mg l⁻¹) applied in the earlier passage stimulated elongation of roots.
The experiments were carried out on highbush blueberry 'Herbert' both on in vi­tro cultures and plants in vivo. In the case of the in vitro study, the modified Zim­merman and Broome (1980) medium was used. For the first subculture dikegulac was tested at a 0.1-10 mgl-1 concentration together with 2iP (5mgl-1). For the second subculture, dikegulac (1-4mgl-1) was added both into medium supplemented with 2iP (10 mgl-1), and into medium without 2iP. In the case of the in vivo study, dikegu­lac (100-1000 mg l-1) was applied as a foliar spray on four-month old plantlets. Dikegulac (0.1-5 mg l-1) gradually slowed down the elongation of axillary shoots in vitro, in the presence of 2iP at a lower (5 mg l-1) concentration. It also retarded devel­opment of adventitious shoots, while proliferation of axillary shoots was unaffected. Cultures grew very slowly when 2iP was omitted regardless of the concentration of the retardant Plants sprayed with dikegulac (1000 mg l-1) solution in vivo developed more lateral shoots which were shorter, and the plants had reduced leaf blades. Cut­tings collected from plants treated with retardant rooted better, compared with the control. Dikegulac may be useful to keep the germplasm bank and in propagation of highbush blueberry, both in vitro and through cuttings.
Plants of ‘Catawbiense Boursault’ and ‘Eskimo’ rhododendrons or ‘Cannon’s Double’ and ‘Kilian’ azalea grown in 4 l containers were treated twice with chlormequat (2000, 4000 and 8000 mg · dm-3), trinexapac-ethyl (50, 100, 200, 400 mg · dm-3), daminozide (2500, 5000, 7500 mg · dm-3), proxeadione calcium (75, 150, 300 mg · dm-1) and once with paclobutrazol (50, 100, 200, 400 mg · dm-3). Shoot length of the subsequent growth flush following the treatments decreased with increasing rates of the growth retardants. The number of flower buds per plant increased with increasing rates of pacloburazol, chlormequat and daminozide. Prohexadione calcium was less effective in flower bud initiation and the worst results were obtained with trinexapac-ethyl.
The experiment was conducted to inhibit the growth of dwarf alstroemeria cultivars 'Rosalina' and 'Dorotea' using flurprimidol and daminozide. Additionally, the effect of these retardants on days to anthesis, flowering shoots number, diameter and longevity of florets was evaluated. In vitro propagated plants, grown in 12 cm pots were treated with single sprays of flurprimidol (7.5,15,22.5 mg l-l) and daminozide (2500, 3500, 4500 mg l-l) following second pruning, when shoots were 9-12 cm long. Well retarded plants of both dwarf alstroemeria cultivars were obtained when plants were sprayed with tlurprimidol at 22.5 mg l-l. Plants treated with daminozide at all tested concentrations were to tall to be grown in 12 cm pots. Flurprimidol significantly reduced the canopy diameter, number of tlowering shoots of alstroemeria cultivars . Rosalina ' and . Dorotea' and floret size of culti var . Rosalina' . Daminozide had no effect on the number of days to flower but flurprimidol delayed tlowering of tested cultivars only at concentration of 22.5 mg l-l. Intensified green leaf colour was observed on tlurprimidol treated plants. The chemical names used: a-(1-methylethyl)a-E 4-(tritluoromethyloxy )-phenyl]- 5-pyrimidine-methanol (flurprimidol), butanedioic acid mono (2,2-dimethylhydrazide) (daminozide).
In fresh moist meadows, double-cut, located in the Bydgoszcz Canal Valley, you can find Ostericum palustre, a species of priority importance, which requires protection in the form of designation of Natura 2000 sites. The aim of the present research was to define the effect of the cutting date on blooming and fruit-bearing of O. palustre in meadows covered by the PO1b agro-environmental programme. Phenological observations included three populations of O. palustre. The first one occurs in a traditionally used meadow, not covered by the agro-environmental programme (2) where the date of the first cut falls in mid June. The second one is located in a meadow covered by the agro-environmental programme (1) where the first cutting date is possible from July 1, and the third population located in a natural meadow is not agriculturally used (0). The present research showed that individuals of O. palustre in the traditionally used meadows (2), prior to the first cut, reach the leaf rosette phase, while individuals which occur in the meadow covered by the agro-environmental programme (1), prior to the first cut, reach the generative tiller phase. Full bloom of O. palustre in the traditionally used meadows occurs after July 20, while individuals of O. palustre in the agro-environmental programme reach that phase only at the beginning of August. At the same time, about August 6, the first inflorescence in 70% of individuals in the agriculturally unused meadow (0) has ripe fruit, and in mid August all fruits are ripe. In mid August 70% of individuals in the meadow used for traditional cutting have ripe fruits of the first inflorescence, and right before the second cut, on August 27, almost 90% of fruits were ripe. Most O. palustre individuals in the meadows covered by the agro-environmental programme do not finish blooming of the first infl orescence before mid August. By the end of August, about 60% of individuals reach the phase of young fruits. The first ripe fruits occur as late as September 12. About September 20 most fruits are ripe. To sum up, delayed PO1b agro-environmental programme meadow cutting, after July 1, does not facilitate the production of ripe fruit in O. palustre individuals.
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