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Carriage of group B streptococci in pregnant women from the region of Krakow and their antibiotic resistance in the year 2008–2012

100%

Brzychczy-Wloch M., Ochonska D., Bulanda M.

Polish Journal of Microbiology

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2013

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tom 62

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nr 4

The aim of the study was a retrospective analysis of the frequency of group B streptococci (Streptococcus agalactaie; GBS) carriage in pregnant women from the region of Krakow, together with an analysis of their drug resistance, carried out between 2008-2012. The study included 3363 pregnant women between 35 and 37 weeks of gestation, studied in accordance with the guidelines of the Polish Gynecological Society (2008). A high percentage of pregnant women who are carriers of group B streptococci was demonstrated. Each year covered by the study, it was in the range of 25-30%, with an average value equal to 28%. The results confirm the need for taking swabs from both the vagina and anus, since 15% of GBS-positive patients showed only rectal carriage. High percentage of isolates resistant to erythromycin was detected, which ranged from 22% to 29%, with an average value equal to 25%, as well as a high proportion of isolates resistant to clindamycin being 17-25%, with an average of 20%. The results indicate the need to standardize the methodology of collecting samples for GBS testing and introduce microbiological diagnostic standards in all gynecological and obstetric centers in Poland, in order to carry out a detailed epidemiological analysis in our country.

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Adherence of group B Streptococci to human rectal and vaginal epithelial cell lines in relation to capsular polysaccharides as well as alpha-like protein genes - pilot study

67%

Bodaszewska-Lubas M., Brzychczy-Wloch M., Adamski P., Gosiewski T., Strus M., Heczko P. B.

Polish Journal of Microbiology

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2013

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tom 62

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nr 1

Streptococcus agalactiae (Group B Streptococci, GBS) constitutes a risk factor for infections of the newborns born by colonized mothers. The adherence of GBS to epithelial cells has been proved to be an important factor in the colonization of mucus membranes of both human rectum and vagina. The objective of the study was to assess the adhesion of the selected GBS strains to the human colon adenocarcinoma cell line (HT-29) and human epidermoid vulvo-vaginal cells (A-431) in relation to the capsular polysaccharides and alpha-like protein genes. GBS strains from the human sources belonging to Ia, Ib, II, III and V serotypes possessing different surface alpha-like protein genes such as the alp 2, alp 3, bca, epsilon and rib in the conventional adherence assay were examined. The adherence of GBS strains to the HT-29 cell line was considerably higher than to the A-431 cell line. For GBS serotype Ia and III, a significant difference between the adhesion to the HT-29 and A-431 cell lines was presented. The adhesion of GBS strains to the HT-29 cell line depended on alpha-like protein genes. The most adhesive ones were the GBS strains containing the rib and alp 2 genes. The adherence of GBS strains to the A-431 cell line depended on both their serotype and alpha-like protein genes. Serotype III adhered to the A-431 cells most tightly, particularly the strains containing the rib and alp 2 genes. GBS strains containing the rib gene adhered to the HT-29 and A-431 cell lines more firmly than GBS strains containing other alpha-like protein genes.

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Streptococcus group B [GBS] - characteristic, occurrence in children and adolescents with type 1 diabetes mellitus

67%

Nowakowska M., Jarosz-Chobot P.

Polish Journal of Microbiology

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2004

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tom 53

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nr 1

Group B streptococcus causes infections in woman during pregnancy and confinement, perinatal infections in newborns related to mothers carrier-state and in adults, mostly in the elderly, with one or more predisposing to infections conditions. Diabetes mellitus is the most common underlying condition. The aim of the study was to determine the frequency of GBS occurrence and GBS antibiotic susceptibility in children and adolescents with type 1 diabetes mellitus. In years 2000-2002 occurrence of GBS in some clinical materials (urine, swabs from pharynx and urogenital tract) taken from 161 diabetics: 90 girls and 71 boys, hospitalized for newly diagnosed diabetes or insufficient metabolic control/longer duration of diabetes and 37 children with hypostatura (control group) aged from 5-17 years, was examined. Susceptibility of isolated GBS strains to ampicillin, erythromycin and clindamycin was determined. GBS were obtained from different materials from 36 (22.4%) diabetic children - 25 girls (27.8%), and 11 boys (15.5%). In all examined groups GBS was detected significantly in children with insufficient metabolic control/longer duration of diabetes (27 of 36 children; p=0.029, x²=4.773). GBS in girls was isolated mainly from vestibule of vagina (25 cases) and in few cases (4) from the pharynx. GBS in boys was grown from materials from urethra (6 cases) and pharynx (5 cases). In the control group, GBS colonization was observed only in one case. All isolates (40 strains) were susceptible to penicillins, however lower susceptibility to erythromycin (3 resistant and 1 moderately sensitive) and clindamycin (3 resistant) were observed. High percentage of carriers of GBS both in girls and boys with diabetes mellitus is the potential risk factor of infection caused by GBS.

Ograniczanie wyników

3 Polish Journal of Microbiology

2 Brzychczy-Wloch M.

1 Adamski P.

1 Bodaszewska-Lubas M.

1 Bulanda M.

1 Gosiewski T.

1 Heczko P. B.

1 Jarosz-Chobot P.

1 Nowakowska M.

1 Ochonska D.

1 Strus M.

2 2013

1 2004

Carriage of group B streptococci in pregnant women from the region of Krakow and their antibiotic resistance in the year 2008–2012

Adherence of group B Streptococci to human rectal and vaginal epithelial cell lines in relation to capsular polysaccharides as well as alpha-like protein genes - pilot study

Streptococcus group B [GBS] - characteristic, occurrence in children and adolescents with type 1 diabetes mellitus