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Moss cushion plays an important role in recruitment of vascular plants. In this study, we examined the effects of water soluble extracts (WSE) of Abietinella abietina on germination of Qinghai spruce (Picea crassifolia) and the effects of moss substrates (A. abietina) on seedling emergence of P. crassifolia in two greenhouse experiments. We found that the WSE of moss affected germination of P. crassifolia in two ways: (1) the WSE reducing the risk of fungal infection to the seeds, improve the germination rate; and (2) the WSE affects germination by its concentration, that is, low concentrations of WSE (i.e., 0.5 mg ml⁻¹ and 5 mg ml⁻¹) stimulate, while high concentrations (i.e., 50 mg ml⁻¹) inhibit it. In seedling emergence experiments, we detected that the moss substrates (both thick and thin mosses) facilitate the emergence of P. crassifolia in dry and intermediate moisture conditions, but inhibit it in moist conditions. In bare soil, the P. crassifolia emergence was also controlled by moisture conditions; the highest (60%) and the lowest (35%) emergence occurred in the moist conditions and dry conditions, respectively. We also found that P. crassifolia seedlings were thinner and taller, but their number higher in moss cushion than in bare soil. Thus we conclude that there is nurse effect of A. abietina cushion on recruitment of P. crassifolia in both dry and intermediate moisture conditions.
Identification of cultivars is essential both in breeding and to settle cultivar disputes. The purpose of the study has been to examine cultivar identities based on absorption spectra of plant pigments and to confirm a genetic stability with SCoT and RAPD molecular markers in new Polish lines of Chenopodium quinoa Willd. Spectral analysis of pigments extracted from plant inflorescences in quinoa gives an opportunity to confirm the cultivar identity and identification of ‘Faro’ and ‘Titicaca’ cultivars and their new lines. Spectral analysis is an effective method of confirming cultivar identity and it should be used in practice for the identification of cultivars or cultivars lines in Chenopodium quinoa Willd. Analysis of molecular markers indicated by RAPD as well as SCoT technique revealed a high genetic stability of the derivative lines of ‘Faro’ and ‘Titicaca’, while variation was detected in plants representing original cultivars: banding pattern different than predominant was present in three plants of ‘Titicaca’ (genetic distnaces from 7.5% to 55.9%) and in a single plant of ‘Faro’(genetic distance 61.2% as indicated by SCoT technique).
In greenhouse experiments, the fungicides containing benomyl (Benlate 50 WP), thiram and carbendazim (Sarfun T 65 DS), or only thiram (Zaprawa Nasienna T Zawiesinowa) were the most effective in protection of meadow fescue, perennial ryegrass and Italian ryegrass against strongly pathogenic F. avenaceum isolate. The seed treatment fungicides containing an azole compound (Baytan Universal 19.5 DS, Premis 025 DS and Raxil 02 DS) showed low effectiveness. F. avenaceum infection of pasture grasses was not controlled by Polyversum biofungicide containing Pythium oligandrum oospores.
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A two-factorial experiment was conducted in 2012-2014 in a greenhouse owned by the Department of Horticulture at the University of Warmia and Mazury in Olsztyn. The experiment had a randomised block design with three replicates. The first experimental factor were six botanical varieties of basil: sweet basil, Thai ‘Siam Queen’ basil, Greek ‘Minette’ basil, purple basil, lemon basil and cinnamon basil. The second experimental factor was container size and the number of plants per pot: 0.7 dm3 container with one plant per pot, 3.0 dm3 container with one plant per pot, 3.0 dm3 container with four plants per pot. In successive years of the three-year experiment, between 22 and 24 February, containers filled with organic substrate (sphagnum peat) were placed on movable tables in the greenhouse, depending on pot size (0.7 and 3.0 dm3). Once-over harvest of basil herbage was carried out between 17 April and 7 May. The plants were cut at 5 cm above the substrate surface. Total yield and marketable yield were identical because no mechanical damage to leaves or symptoms of leaf diseases were observed. The content of dry matter, total sugars and L-ascorbic acid in basil herbage was determined. Basil ecotype had a significant effect on leaf greenness index, fresh herbage yield, dry herbage yield, and on the content of dry matter and total sugars. Growing basil plants in 3.0 dm3 containers, with four plants per pot, contributed to a significant decrease in fresh and dry herbage yields, in comparison with the remaining treatments.
In five monitored farms, stem base rot symptoms and carnation wilting were observed on 10–35% of plants and Rhizoctonia solani was the most frequently isolated species from diseased tissues. Additionally Fusarium avenaceum and Botrytis cinerea were only rarely noticed in the diseased stem parts. Isolates from carnation and eustoma caused stem and leaf blade rot of carnation but tissues were colonized significantly faster by a culture from a host plant. Different reaction of three carnation cultivars was observed on R. solani inoculation. The optimal species growth was observed at 30°C and it was also the most favorable temperature for colonization of carnation stem parts. In the greenhouse trial isolate of R. solani obtained from carnation crops caused stem base rot already after 2-week-growth on 1 per 5 plant in each replication and within the next 2 weeks 4 per 5 of plants died.
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