Caveolin--1 is a multifunctional protein and major component of caveolae membranes that participates in regulation of signaling pathways, endocytosis and molecular transport. Caveolin-1 takes part in regulation of angiogenesis regulation signaling pathways. Diamond nanoparticle have been shown to inhibit development of blood vessel. Molecular mechanism of diamond nanoparticles anti-angio-genic activity can be related with interactions with cellular membranes. The objective of this experiment is to verify effect of carbon nanoparticles on morphology of highly vascularized chicken embryo chorioallantoic membrane (CAM) and caveolin-1 intracellular localization. In this study two types of carbon nanoparticles were used: diamond nanoparticles (ND) and graphite nanoparticles (NG), which are similar in size (3-5 nm) but different in molecular structure. At day six of chicken embryo embryonic development sterile implant of diameter 10 mm made from Waterman filter paper ware placed on chicken embryo CAM. At day seven of embryonic development implants with CAM were subjected to further analyzes. CAM cross-sections were immuno-localized with anti caveolin-1 antibody and visualized by confocal microscope. Three dimensional analysis of chorion membranes show that ND, but no NG change intracellular distribution of caveolin-1. Furthermore ND decreases density of mesenchymal cells and extracellular matrix collagen fibers
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